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BIOSTIMULATION THROUGH LASER RADIATION AND BIOPLASMA
BY V.M. INYUSHIN AND P.R. CHEKEROV
TRANSLATED BY
Scott Hill and T.D. Ghoshal
Copyright on Russian original text is held by the Kazakh State University,
Alma-Ata, USSR, 1976. All rights to this english translation, and any
other translations derived from it, are held by the Danish Society for
Psychical Research, Copenhagen 1976. No part of the manuscript may be
duplicated or otherwise distributed without explicit permission of the
translators, except for short passages which may be quoted for review
purposes. For information on distribution, write:
THE DANISH SOCIETY FOR PSYCHICAL RESEARCH
POSTBOKS 1168 DK-1010 Copenhagen K, Denmark
Best Copy Available
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ouncing a limited edition English translation of an important new
{from the USSR ----- first printing August, 1976:
:
STIMULATION THROUGH LASER RADIATION AND RIOPLASMA
..
BY V.M. INYUSHIN AND P.R. CHEKOROV :
-
KAZAKH STATE UNIVERSITY, VISSR
TRANSLATED BY:
SCOTT HILL, UNIVERSITY OF COPENHAGEN
AND
T. D. GHOSHAL, COPENHAGEN NAVIGATION SCHOOL
-
ntents:
Author's note
page 3
Briefly about lasers
6
Unspecified action of laser rays
on living organisms
11
Physiological action of monochromatic
polarized red light on peripheral
blood and blood-forming organs
24
The reaction of endocrinal glands
during radiation by monochromatic
red light
28
Stimulation of regeneration. by
monochromatic red light
37
A word about bioplasma
49.
Biostimulation through laser radiation
through acupunctural points
75
Conclusion
105
Bibliography
116
:roduction:
Optical quantum generators (lasers) are one of the most significant
Lievements of technical throught during the second half of the 20th
itury. In this book are presented the physiological action of
liation by helium-neon lasers. Data is given on the dynamics of
'siological and histochemical properties of tissues under the influence
laser radiation. Light is shed on the little-known aspects of using
er radiation clinically as a factor for stimulating the nervous
stem during treatment of neurological diseases and diseases of the
sor system. Special attention is given to presenting the problem of
bioplasma connected with the treatment of the action of laser light.
-
s monograph is meant for doctors, tecnicians, teachers of biology,
lical and agricultural students, veterinary surgeons, medical
stitutes, biological faculties of universities, and research workers.
-detatch here
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Attn:
Mr. HILL
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INTRODUCTION TO THE ENGLISH EDITION BY THE TRANSLATION EDITORS'
THIS BOOK, Which was printed in the USSR in the spring of 1975
fan edition of only 4000 copies) contains the latest information on a
number of research fronts: Kirlian electrophotography, acupuncture,
research into the existence of "bioplasma", and the new science of
biostimulation by ultra-weak laser light: : Brief mention is also made .
of other research, we can call parapsychological, which the soviets
call psychoenergetics, and of heliobiology, which is the study of
rhythmic functions in the biosphere, and their supposed extraterrestria.
(i.e. solar) causes.
Although in the original monograph there are only 115 pages,
because of the complexity of the material, it has taken us over a year
to translate and edit the material. ... Why have we taken so much trouble,
especially since we have not. had the backing from any publisher of
financial sponsorship of any business interests ?-
It is our opinion that the material in this monograph is of utmost
importance to scientists around the world. The only one of the fields
covered in this book which has been at all circulated in western
scientific circles is the Kirlian process --- due to the interest of a
few parapsychologists who have used the technique for psi experiments.
Even this material-as is revealed in the conclusion of-this monograph
has been misunderstood, garbled, misrepresented, and lightly dismessed
by the scientific establishment. " One reason is the misinterpretation
of the concept of: "bioplasma": which is the pervading theme of this book
since it is often invoked as the underlying medium of energy exchange
and transformation .in biosystems . . Although there may be some doubt
about-the "proof" of the existence of "bioplasma", in this work Inyushin.
and Chekorov give the best theoretical description to date of the
-
composition of - "bioplasma"; and the-experiments which they feel point
to the existence-of such a substance. .
Certainly the most. neglected area covered in this material is
the problem of . "weak resonance blostimulation. " Since this material
is not primarily parapsychological-in nature, it has not been picked-
up by the parapsychologists who so eagerly reported on electrophotogra-
phy. Nor has it been picked up by the scientists who should be most
interested'in it: the-laser scientists, medical : doctors, and biologists
in the west who pioneered -in. the application of high-power lasers in
medical treatment (1, 2) . In fact, western scientists seem completely
-ignorant-that there are any effects at-all due to non thermal
influences of light on biosystems. The greatest part of this book
is devoted to the problems of biostimulation by laser (and other types
of) light.
While . it has been known for some-time that the Soviets had an
intense interest in acupuncture, it has not been clear until now
exactly what they were doing. . In this book; the longest chapter is
devoted to biostimulation through acupunctural points, and a consider-
able amount of clinical data is presented for the first time, A
fascinating addition is the use-of weak light stimulation at acupoints
in addition to the usual needling . techniques.
One of the most interesting scientific turn-abouts of this century
has been the sudden change in the status of-the so called-mitogenetic
radiation, discovered by the soviet scientist A.G. Gurvich in the
1920's .. A: survey of the- Gurvich controversy up to the year 1949 is
given by the dutch .scientist S.w. tromp in his book "Psychical physics"
(3), and we-have brought .this up to date from other sources. Although
some scientists.were able.to reproduce -Gurvich's experiments with
"living detectors", others failed, and attempts at using sensitive
light detection systems failed also.
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Gurvich's work fell into disrepute both within and without the USSR.
As late as 1960, a doctoral dissertation by M.N Moyceyeva totally
rejected the existence of mitogenetic radiation. It was not until 1966
that V.S. Konev et al (4) succeeded in showing that the radiations from
the cell cultures was so weak in the UV band that it could easily be
absorbed in the apparatus which was used to measure it! (for example, by
using glass lenses instead of quartz). In 1974 A.A. Gurvich published
results of an experiment with hops, point out the errors which had made
the existence of mitogenetic radiation doubtful in the intervening 50 years.
Prof. V.P. Kasnacheyevat the Leningrad Brain Institute, since 1967 has
been doing experiments in "cell communication" in the UV band, which stem
from the original Gurvich work (5) The Czech professor Sedlatchko has also
replicated the work, according to accountswe have received. (these will
be translated separately by us and can be ordered using the form in the
back of this folder).
Apparently independently of the latest wave of Soviet change of mind,
two western researchers, Quickendon andHee (6) in 1973 successfully
replicated the Gurvich effect with yeast cells, using sensitive photo-
multipliers in the UV band and quartz windows.
This research underlies the whole thesis developed by Inyushin and
Chekorov in this monograph --- that light in biological systems plays an
organic role, and can be measured by appropriate instrumentation.
In the meantime, we have received supporting documentation from the
USSR and elsewhere regarding verification of some of the research results
presented in this monograph, and giving further technical and clinical
details. This material will be available shortly for distribution.
A few
words about practical matters regarding this translation:
In some cases, where there is doubt about the meaning of the russian
word, we have given more than one . translation, the second or third
meaning being given in parenthesis (brackets). Where the material is
especially difficult, we have added occasional footnotes at our discretion.
In a few cases, we have omitted redundant passages, but these are clearly
marked in the text. . Immediately following the conclusion is the translation
of the original Soviet bibliography given by Inyushin and Chekorov.
Immediately following this is a western bibliography compiled by us, which
fills out the gaps in the original references, giving articles in
English and a few other languages on related research.
We are very interested in receiving constructive feedback on this
manuscript and for this prupose have included a form at the back of the
folder. If you find any errors, spelling mistakes, etc. use this form
to tell us about it.
Scott Hill
T.D. Ghoshal
Copenhagen, Sept 1976
F
References :
1) Hias, Scott: "Acupuncture Research in the USSR" American Journal of
Chinese Medicine, 1976, Vol , Kr , pp.
2) Barnes, Frank: "Applications of Lasers to Biology andMedicine"
Proc. of the IEEE, vol 63, Nr 9,1975 (interesting for its omissions)
3)Tromp, S. W. "Psychical Physics", Elsevier, Amsterdam 1949
4) Konev, V.S. "Flourescence and Phosphorescence of Proteins and Nucleic
Acids" ( English Trans) Plenum Press, New York 1967
5) Journal Psychoenergetics Systems, 1975, Nr. 1 Vol 1, pp. 37
5 ) Hee, shane and Quickenden, Terence: "Weak Luminescence from the yeast
Saccharomyces Cerevisiae and the existence of mitogenetic radiation"
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A WORD FROM THE AUTHORS .
Above all, It is our. wish to explain to the reader how the Idea-
of resonance biostimulation appeared and why it is connected-with-
-
bioplasma. Here we wish to reminesce a bit of the past: More than
50 years ago professor A. G. Gurvich discovered the existence of
mitogenetic radiation (rays radiating. from cells during their division
In the UV band of the spectrum). An amazing fact was arrived at, viz.
that single photons were able to make the quiescent cell divide. In
its turn, the dividing cell stimulates the neighboring-ones with its-
rays to undergo .a . process of cell division.
Many theoretical works on the problem of mitogenetic radiation
have been published, but not a ' step has been taken for their prac-
tical utilization.In.the fields of medicine and agriculture. The
mechanisms of intercellular ray reactions remained a puzzle, there
were no clear picture of the physiological role of radiation. There
were no good experiments to extablish the existence of the biofield.
(A.G. Gurvich, 1944). -
.
We shall . fully. explain the turn such a crisis took. It was conn
ected with the unsatisfactory level of development in certain branches
of physics, the weakness in the development of biophysics. The chasm
between physics and biology existed- . - for a long time and prevented-
ideas from meeting, as the experience of the natural sciences_shows.
A whole series of new facts -, theories, and separate courses of
development came to life. . Enthusiasm for the analytical aspects of
biology was also a reason for "a cool attitute" towards problems which
A. G. Gurvich began developing. The very essence of the work of the
school of A.G. Gurvich was to demonstrate that-with the aid of-radiant
energy_of_low_intensity it-was-possible to control_ biological_processe
This, on the face of it, is the phenomenon of resonance. When the
space-time: parameters:ef. the-radiation of the donor and the recipient
(in the case of their convergence) induce this or that process. Even
an insignificant change in these parameters such as, for example, -
the frequency of radiation, brings about-the-deterioration of-the-
effect. Consequently, resonance-biostimulation is an_amplification or
actually" the acceleration of metabolic processes under the influence
of the radiation of definite parameters (wavelength, polarization,
modulation, Intensity, etc. )
t
0
5
1
4
In the very core of the experimental material amassed by the
Gurvich school ,the idea of resonance-biostimulation-took-its-form .-
The idea_got its_concrete development_at_the_faculty_of_biophysics
of the Kazakh- State University and.has gained the successful approbati
(approval). under_clinical conditions. It is neccessary to mention
that the regulation of processes based on the mechanism of resonance
biostimulation are only possible with the knowledge of the energy
structure of the whole organism, since, in fact, the whole constitutes
the norm of all the countless processes taking place_at_every_second,
in the living organism .. As is known, biochemists and biophysicists
already know a lot about the properties of molecules, comprising the
organism., A new branch . has appeared, that of molecular biology,
however one shor Id not forget that the molecular level represents
but one of the levels of organization; and life is a tightly
intertwined system of different levels governed by_the laws of the
whole .. . As the Nobel prize laureate, A. St ... Dierdri remarked:" "The
composite level is the most . complex and difficult to study. It is.
namely at this level that the fascination and capriciousness of life -
phenomena manifest themselves. ". It is difficult not to agree with-
the words of this famous codentist."The discovery of bio-energetic-
processes conditioning the entirety of the living organism is the
key to understanding many problems.
. .
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1WORD FROM THE AUTHOR
- 2
And when, in our book, we speak of the possibility of directed Influenc
on the organism, INa pathological . state, we depart from. the original
concepts about bioplasma. Bioplasma is a plasma(4th state of matter
In physics) under conditions present in the living organism. It is
namely through it that the resonance action of radiation of defined
. Such radiation. can only be obtained
parameters is realized;
withthe aid of: lasers, that is, optical quantum generators. In the
U.S.A., England, France high-power. lasers. are. successfully being
applied in surgery, ontology, etc. The Kazakh State University is the
pioneer institution in the utilization of the laser radiation of low
intensity in biology an medicine. A completely new sphere of applying
radiation ergy to control the reaction of changing propertes has been
achieved. The idea, as we have. mentioned above, has its historical
roots. in our native science. Here we may add that in the fiel of che-
mistry, successful experiments in controdling shemical reactions with-
the aid of gas laser radiations based on resonace -- interaction between
the radiation and molecules have successfully been carried out.
(N.G. Basov; etal, 1973). It is obvious that the resonance effects of
stinulation are not characteristic, or special for a living organism.
At the biology faculty of the Kazakh State University named after
S.N.Kirov research made on resonance biostimulation with the aid-of-
light, on biological processes were-begun-in-1965. Since that time,
9 years have elapsed. During that period, experimental_work has_been
supplemedted by scientific.and practical. bodies: . the. Alma-Ate state
medical institute, the "Aksay"Republican child clinic of the ministry
of health. of the Kazakh SSR, the republic. clinical hospital of the
ministry of health of the Kazakh. SSR, the
town child hospital of -..
Alma-Ata, the Kazakh scientific research institute of tuberculosis-
the Tselinograd medical institute, the Lvoy medical institute, the
Moscow medical institutendand others.
The results obtained show that biostimulation. with the aid of laser
lighet holds .many practical .applications.
The significance of resonace biostimulation methods and the problems-
of bioplasma were widely discussed-at-the republic conference "Proble
of the bioenergetics of the_organism_in_normal_and_pathological state
which took place in Alma-Ata in May 1971.
The time has come to sum op. the research presented, and theoretically
ponder over the experimental material. Such has been our task in writi
this book .. Many sections were written on a discursive level. The
theoretical opinions are often-Original and may evoke objections and
criticisms.
This is quite understandable, since we are
confronted by a co pletely. new. sphere of medical and biological scien
where, as yet, there are no established concepts and obvios theses,
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---
1
:5.
A FEW WORDS . ABOUT. LASERS.
A lot has been written about lasers, predominantly about the
high-power ones,
-since the low-powered gas lasers were for a very.
long time not utilized in biology and medicine. In the meantime the
family of lasers is-growing. .. Eventually; thanks to lasers, it was
possible to achieve-a resonance biostimulation of processes taking
place in the organisms of living beings and man. That is why we have
thought it an idea to describe their construction and applications in
-biology and medicine .:
-
Optical quantum generators (00G's) received . the acronym Laser-
1
The word "laser" is made up of the first letters of the English
words: light: amplification by stimulated emission of radiation.
Depending upon the nature of the light generated, lasers can be divided
-
into three basic groups: gas and ton, whose stimulation is brought
-about-by an electrical charge, those with an optical pumping on the :
crystals, glass, liquids, and plastic; and semiconductor lasers.
Gas lasers: Gas optical quantum generators give the most
monochromatic light: radiation. At present, progress in the constructic
of gas lasers has gone very far~Gas optical quantum generators have
been constructed that radiate right on hundreds of different wavelength
ranging from ultra violet to infra red.
Let us examine the principle of construction of a-gas He-Ne laser
which is operated continuously. : The basic active element of a gas
OQG is the discharge. tube. Within the tube is a cathode and an anode,
the tube itself. if. filled with a mixture of helium and neon. The
partial pressure of He amounts . to .about . 1.mm Hg, and the Ne of 0.1 mm
Hg. Under the Influence of high pressure, a luminescent discharge .
takes place in the-tube. The generating source of radiation is Ne,
however the stimulation of atoms is achieved with the aid of the He
atoms. , The stimulated He atoms; colliding with the Ne atoms give off
energy to the latter, which is indispensable for them in turn to
Ittain an excited state. In this way, an active medium is created in
the tube; composed of Ne atoms, possessing inverted populations in
the electron energy levels. "_The-spontaneous-radiation-of-the-
separate_Ne-atoms_brings_about_a_dispersal in the active_medium of
notons, corresponding to the electron displacements in the neon atoms
rom 3 S to 2P level. . In the He-Ne gas laser one of the electron
Isplacements
353 - 2P is used. Further, an induction of coherent
adiation of other excited Ne-atoms takes place.
The chain reaction of this process is insured by the tube being
laced in a mirror resonator. " The frequent_path_the light takes down .
long the ast,s .of the discharge tube brings - about the formation of a
. powerful current. of Induced, trained; coherent radiation, character-
stic of a gas laser. Such multiple paths taken by the beam is achieve
ith the help of reflecting mirrors. The discharge tube is enclosed by
ane parallel glass plates at each end,- fixed at a definite angle to
le tube. (i.e .the Brewster angle -- Eds ). Ne radiation takes place
rough these-windows without reflective loss. The mirrors of the
sonator are covered by many dielectric coatings. They possess :high
flectivity, 98-99%, and consequently, absorb very little light.
ergy, passingthroughthe mirror. The transparence of the mirrors is
timal for the laser being able to -emit a powerful current of-radiat-
1 without noticeable: decreases in the mirror resonator in which the .
stem of standing light080PECAGRDP9600787R000500080001-0
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! different types of generators presented in table one are used In
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A WORD ABOUT LASERS ---
2
30270 State Lasers
4
-In ruby lasers, polished ruby rods are used. For optical pumping,
an impulse lamp is used. For the generation of light of a given
parameter, the laser has a resonator. In the simpler types, the
resonator is represented by two semi-transparent mirrors placed towards
the periphery of the active medium. The
. mirrors force the photons
to disperse perpindicularly to their surface, and frequently pass
through the amplifying medium.
This causes the appearance of new excited particles in the medium,
Subsequently, the whole totality of photons is amplified anew, when
they pass between the mirrors. This process brings about the appearance
of a powerful directed light impulse in a narrow waveband interval.
Since one of the mirrors is semi-transparent, a very strong current
of brilliance goes through it in a perpendicular direction in relation
-to-the-mirror-surfaces. All the quanta radiated strictly correspond-
in phase and frequency --- i.e. they are coherent.
¥
The solid-state, ruby impulse laser has found wide applications in
biology and medicine. The radiation generated by a roby laser has a
Wavelength of. 6943 A, the power of radiation may vary within wide
Hults. The highest light impulse energy was obtained in the region of
1500-joules. The utilization of microscope lenses-enables the area of
the spot to be reduced to tenths of a square micron. At the present
time, a constant plan of work for ruby generators has been worked out.
The radiation energy of such a laser comprises a few milliwatts (mw) .
With the help of such a laser it is possible to obtain non-thermal
-biological effects of radiation, on a wavelength of 6943 A. ... However,
-the-radiation area of solid-state lasers is larger than gas lasers.
Solid-state and gas' lasers generate light on definite wavelengths.
Diagram I shows the area of the electromagnetic spectrum from 300-
1200 A, that is, from the ultra-violet to infra-red band. The emission
lines are many, but there are also blank spaces. (i.e. the
so-called forbidden transitions --- Eds). In connection with this the
-fact that for purposes of spectroscopy, chemistry & biology, a very
accurate calibration is required for this or that line of absorbtion.
New types of properties were discovered in quantum electronics, with
the aid of which it was possible to cover the
whole band of the
light spectrum and obtain the generation of radiation on any wavelength
In the band sector. Such properties were discovered- -- they turned
-out to be organic-dyes. Up to the present time, the generation has
been discovered in several hundred compounds
In the institute of physics of the academy of sciences of the
Belorussian SSR, a dye laser called "Raduga" (Rainbow -- Eds) was
constructed. (V.I. Stepanov, A.N. Rubinov, -1973) . Its layout has
been shown in diagram 2. The radiation of a ruby laser (1,2, 3,4)
takes place through a cylindrical lens (5) and a non linear crystal (6)
and doubles in frequency
Further, the light current of doubled
frequency, wavelength 3470 "A, is focussed on a dye chamber, in the
form of a narrow stripe, stretched across the lower part of the
cuvette axis, In this way, the pumping of the dye is accomplished.
The generation of radiation takes place in a perpendicular direction in
the resonator, formed by the mirror (lo), and the diffractional grating.
(8). Ten grooves, filled with solution of various dyes, have been
lesigned in the apparatus. The grooves are fastened in a special
evolving cylinder (drum) which enables the rapid change-over from
ne dye to another. The easy shifting of the generation line is
Stained by the simple rotation of the diffractional grating (8).
uch a laser enables the attainment of light impulses ranging in
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(
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A MERS ABOUT LASERS - 3
power from hundreds of kilowatts to a few milliwatts, and in any
wwwband-from-3600 A to 10, 700-A, the spectral line being of a width-
of is to 50 A. In the not-to-distant future, biologists will have an.
Incprwent for the induction of widely-differing effects during
misslation and inhibition of metabolic processes. Even now, lasers
"have been constructed based on dyes, inwhich a rapid spectrum change-
very: Is achieved in lo seconds. Such a property of a laser will
doubtlessly find a wide application in the rapid spectroscopy of
Slo-cbjects, above all in diagnosis.
-
Semiconductor lasers
Up to the present time, generation has been achieved on 24 semi-
conductor , crystals. During the bombardment of semiconductors with
beams of fast electrons, electron-hole pairs are created in them. The
high coefficient of amplification
in semiconductors allows the
- creation of an optical quantum generator of a large surface area of
-
radiation-in the form of mirrors. At present, semiconductive lasers-
are being turned out by our industry
F
The main element of a semiconductor OOG is the semiconductor
diode with 'a thickness of o.1 mm and an area of. a few mm ... The plate
has contacts bonded on two sides. The plate itself is made us of
two dissimilar parts, comprising an electron (N-type) and a hole .
(P-type) conductance .- The gap difference between them is called the
P-Y junction. The thickness of the penetration region is somewhere
around a thousandth of a mm. It is in this area that the generation
1
of radiation also takes place. If, an electrical potential is placed
"on the diode, then it will lead to the aggrandization of the
Tecombination processes and the liberation of energy in the form of
-light quanta. Semiconductor-diodes of Arsenic & Gallium (GEAS ) CAN BE
generated with an impulse of high-level current_and_in_a temperature
of liquid Nitrogen. At present, generation has been obtained at
room temperature as well. The efficiency of the diode is high and
"theoretically approaches /o%. The miniture dimensions of the lasers,
the ease with which light radiation can be madulated, the different
spectra of generation, will undoubtedly in the future make them-into
convenient apparatus for photobiological action. Our industry produces
a semiconductor laser of a wavelength of 9100 Å and a power of
1 watt.
The most monochromatic light is generated. by gas lasers. . .. The
generation of monochromatic polarized coherent light with the aid
of gas optical quantum generators permits the selective activation
of_metabolical_processes in plant organisms, animals and man.
However, research into the specific effects of laser light during its
iction on biological objects did not begin suddenly. How the amassing
if material on the biological action of laser radiation took place
he reader will be able to discover in the following pages.
...
:
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He-Ne
. I: spectral lines generated
wer on various active mediums
'3303, solids X-axis : MKM
-
0,5
0,6
0,7 0,8
0,9
1,0
1,1
1,2 MK and wounds.
Рис. 3. Внешний вид гелий-неонового лазера, оборудованного
для облучения костных переломов и ран.
Спектральные линия, генерируемые лазером на различных
ANTHRAX cnenax.
: II: diagram of an optical quantum
or "raduga" (rainbow) (1) ruby las?
"csonator mirrors (2) &-value
or of ruby (Q-switch !: ) (3)impulse
flas. ) lamps (4 (#uby crystal
i :drical lens (6)nonlinear crystal doubeling the frequency of the
on of the ruby laser (7) filter absorbing the constant frequency
8
3
.
7
O
347 AM
2
694 MM
4
0
3
1
10
Рис. 2. Схема оптического квантового генератора «Радуга»:
Kana DeSONarona
рубинового лазера: 2 - модулятор добротности рубинового лазера: 3 - импульсные лампы накачки
ного лазера: 4 - кристал. рубина: 5 - цилиндрическая линза: 6 - нелинейный кристалл, удваивающий частоту излучения
кого лазера: 7 - фильтр, поглощающий неизмененную частоту; 8 - дифракционная решетка, поворот которой дает плавную
ойку линии генерации и ограниченной области: 9 - револьверный барабан с 10 кюветами, заполненными растворами раз-
личных красителей: 10 - выходное зеркало лазера на краситель.
raction grating, wien rotated, gives shift in generation lines within
od range (9) revolvin - ds
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Diagram 3: External view of l'e-Ne
laser for radiation of bone fractur.
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*CEUTIATNO: UNSPECIFIED ACTION OF LASER RAYS ON LIVING ORGANISMS
Immediately after the creation of the first optical quantum
separators (00G) research work was done on the possibilities of
wing them for biological or medical purposes. As is known, lasers
ent radiation at various frequencies including visible or red
Ilest. The first lasers were made for the red part of the spectrum.
is this comection, the biological effects of laser radiation were
-post-studied in the red light spectrum. As yet, little is known
about other divisions of research. At the present time, ruby and
He-Ne lasers are the most widely used in biology and medicine.
The material presented gives an idea about which routes researchers
tock after new sources of light appeared, sources of high intensity.
7: 15 Rhown that one of the characteristics of laser light is not
only-its-high efficiency but also its characteristics-of-monochromatic
and polarized radiation. Another characteristic of laser light is_
that it can be produced by short impulses, such discharges can
setain large power values. As a result, the action of the powerful
pulses on biological objects creates the phenomena of a locally
contained thermal effect. The thermal effects of laser radiation
are-widely applied today in medicine .- - A great deal-of-research-has
been devoted to finding out the action of ruby lasers on organs of
sight, Thus, lasers have found wide application as_photo-coagulators
for the treatment of swellings, exfoliation of. the reticular membrane,
and other diseases of the eye. Concomitantly, experimental work was
carried on. Opthalmologists converted the high power lamp in'
a ophthalmo-coagulatory apparatus into a ruby laser and obtained-good
curative effects. A.F. Migacheva (1969) carried out experiments_ on_
more than 70 rabbits of the strain chinchilla. Radiation was effected
with the aid of a ruby laser whose wavelength was 6943A, the length
of the impulse was 10
seconds. The radiation energy on horny
material was 0.002, 0.02, 0.1 and 1 joule, respectively. It was
observed that at this energy level of radiation at 0.002-joules-on --
the center of nadi, or focus of infection, the total destruction of
the pigmented cells as well as the segments of the rods and cones
of the retinal membrane could be seen. Layers of nerve fiber
K .
1
ti
were observed. At a period of lo days after what was happening at
The nadi, there was an absence of the layer of the rods and cones
-and the part of the nuclei-of the external-necleic layer .-- In -- the
-remaining layers har ly any distrophic changes in the perinuclear ende:
and lysis of nuclei were observed. With the increase of radiation
energy naturally more wide and deep damage was noted. Over a period o.
thirty days, the locality of damage per presented itself as
being an atrophic pertoretinal groove (ridge) containing chologenic
fiber and pigment .-. In the retinal envelope, surrounding the ridge-
distrophal changes were observed. Data found in literature confirmed
that the action of OOG rays of wavelength 6943A, impulse length
lo **
sec, energy range 0. 02 -- 1 joule, creates changes in picmentary
epithelia of optical and vascular envelopes and the degree of
damage is directly proportional to the amount of energy given,which
penetrates the eyes. In other section of the eye around the epi-
-center of damage hardly any chain of distrophal changes occurs. This
particularly applies to the tissues in the eye. Some researchers
observed the absence of any damage to the tissue of the eye during
application of OOG radiation applied with the purpose of coagulating
the iris envelope. (L.A. Linnik, L.A. Vedmedenko, and others, 1969) .
-Those showing the recurrance of damage around the zone of-
-coagulation are M.B. Chilik, I.K. Kerova (1969). In the same period,
I.B. Butman (et al, 1969)_observed possible_damaging_effects of
laser radiation on organs of sight. In the opinion of the authors
cited, the unfavorable action of laser light is increased in condit-
Ions of low illumination (ambient lighting levels)
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Such is the action of small amountsof stain combined with laser ray
action. When radiation is effected through a special microscope,
reports N.F. Gamaleya, a : selective disintegration of the mitochon-
drial cells of the cardiac muscle by the impulse action of an argon
laser is observed, Such selective disintegration of the mitochrondri
is on account of the presence of cytochrome-C, which-absorbs green --
light.
Many experiments have been devoted to changes taking place
In the skin under the influence of laser radiation. At 30-50 Joules
energy levels, skin damage took place which manifested Iteself in the
-form of a vesiele with an exudatory inflammatory reaction and the
appearance of a rind with successive . inflammations . (decomposition).
The majority of radiated hamsters survived. In those that died,
Intensive damage to their bodies was. found ... Usually, they were
hembrages and edema (dropsy) in the ventricular walls and in the
inner organs, stomach and Intestines. As far as the ventricles are
concerned, situated between the damaged-zone on the ventricular wall
and_the_damaged internal organs, intestines and stomach, no visible-
changes whatsoever were noticed.
Histological investigations made on the damaged skin showed
distinct differences between the living and non-living cells. Hair
follicules retaining their pigment and those having lost them were
clearly visilbe. Vesicles appeared, after the action of laser rediat-
ich_and_were primarily distributed in the epidermis.
Numerous investigations have been made to discover
the
action of ruby laser radiation on the organs of ventricular cavity, th
chest, the pelvic areas, and.so on.
When ruby light radiations of great intensities are applied,
necrotic-phenomena are observable. Thus, radiating the heads of
=ica_at_energy levels below loo Joules brought about the death of the
majority of the animals, within a period of 24 hours after radiation.
Liceding from the mouth, nose, and ear channels and eye orbits was
alserved. Intercranial hemmorages - . were visible in the area of the
"negtal distributions, stomachs, and conductive (circulatory)
!
channels .- No-noticeable morphological changes were observable-in ---
the bone tissue. However, the marrow cells_had lengthened-and-nuclei,
condensed. In this way, investigations_have_shown_that_at_energies
: Lader loo joules, wavelength. . 6943 A, about 10% of the energy
"La!jede penetrates the skin, muscles and cranium. It is neccess
":" to note that the above-mentioned results were obtained on mice,
watto radiated-skin-sectors were-shaven clean of fur. Where fur was
:, damage to the brain was less considerable.
think it is possible to limit ourselves with the factual
demonstrated showing the varous thermal and non-thermal effec
"radiation. " The above-mentioned compiled. data sufficiently
crates the manifold biological effects brought about by
radiation: According to the opinion of E. Klein and S. Fine,
echanicms of laser radiation can be seen- - from the vantage-
e cf two hypotheses:
purely thermal hypothesis based on the investigation of the
In where and in vivo. According to this hypothesis, the effe ts
Lation as far as they pertain to the cell are basically not
font-from the effects of an ordinary thermal heating.
a hypothesis "of many factors", according to which-
-various
are considered to be of primary importance with the exception
casing the temperature during radiation.
degree of the domination of. the various. factors depends on
TT/ "and Intensity of the incident radiation, and is determined
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radiation. The most important parameters of radiations are in the
form of energy, energy level, power and intensity, wavelength, etc.
The physical parameters of biological systems having significance
are: reflective properties, absorption properties, thermal conductivity
and mechanical properties.
A.A. Gorodetskiy reports (1966) that the light of a ruby laser
can bring about a free-radical state. The action of the focused laser
radiation was studied by them on freshly prepared human blood samples.
The samples were then subjected to staining according to the
Romanovski-Gimze method. Damage to eritrocytes was discovered, the --
leucocytes remaining undamaged The hemoglobin of eritrocytes is
especially easily damaged (disintegrated) by the radiation impulses
of a ruby laser.
During investigations made of the EPR spectrum of a whole see
series of biological samples, a series of noteworthy facts were
revealed. An initially shaven skin surface of animals was subjected
-to-in vivo as well as in vitro radiation. Soon after radiation
these sectors were cut off and placed in an ampule for EPR analysis.
Pigmented and white skin was analysed applying the EPR method.
Un-radiated skin, white as well as pigmented, did not give out any
EPR signals. In the radiated pigmented skin, EPR signals were Tec-
orded. (EPR - electron paramagnetic resonance -- - Eds) .
An-EPR-signal was also observed in the eritrocytes of pigeons
after their subjection to laser light radiation". Consequently, the
activity of laser radiation on biological tissues leads to the
appearance of free radical states connected with- pigment clusters.
Investigations have also been made on human skin. The radiation
cf-skin samples was. carried out with the aid of a ruby laser. It
was discovered that only the pigmented skin of a human being gives
of a stable EPR signal, The nature of this signal is connected with
the pigment --- mellonin. One should not ignore the possibility of
the participation of multi-photon processes in the biological effects:
of radiation. (L.B. Rubin, 1969) .
-In the optical waveband (visible light), one should expect a
considerable-intensity of-multiphoton-processes-from organic
molecules, insofar_as_they possess wide_intensive absorbtion areas in)
that part of the spectrum. A definite probability exists that under mo
the action of laser light beamed on biological objects, multi-photon
processes will take place.
US!3 D
"According to the . opinion of L.B. Rubin (1969) , an experimenta.
nfirmation-of-such-processes will be of considerable-significance.
the following considerations: it is a well-known fact that
n in the visible waveband sectors, light quanta are of extremely
ried quality from the point of view of the biological effect that
produce (extremely different qualitatively) . For example, red
tt does not show a large effect on the photosynthesising tissues,:
ive- and-violet light can bring about some suppression of life activit-
ultraviolet light in the region of 2,500-2,600Å absorbed by nucleic
brings about the death of cells .. However, under the action
! laser light lethal consequences can take place, both at 10, 600
4
well as 5,300 A. As a matter of fact, the four-photon
250
ration of a neodymium laser radiation, .. . 10,600 A or a two-
shoton-absorption of its second harmonic, 5, 300 A will be equal to
the action of I quantum of wavelength 2,650 A. Consequently, in
experiments_and evaluations of data obtained, it is necessary to take
into consideration the possibility of the existence of multiphoton
At present, intensite research is being made into the reaction:
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- s .
After radiation Hela cells with the aid of a ruby laser, change
in the chromosomes of their tissue structure were noticed, which
were morphologically convergent with the changes in the nucleus
after X-ray radiation. (S. Fine and E. Klein 1968) The process of-
cell division arrested in the prophase of human leucocyte cultures
subjected to ruby laser radiation, are described. After chicken
embryos were subjected to impulse ruby. laser radiation, abnormal
phenomenon were observed through the shell. The extremities of the
embryos became deformed, and a lumpiness was observed in the
internal-organs
There have been reports of the possibility of damaging inner
cell organelles in the culture of the myocardic tissue, with the
aid of microwave argon laser. Therewith, a collective destruction
of mitochondria in living cells of the tissue culture can be observe
On going through the experimental data concerning the action of
neodymium- - and-ruby lasers on tissue culture. .. - cells, the majorit
of authors_come to_the_conclusion that most rapid damage develops
in the membrane structures of mitochondria- the endoplasmatic
reticule (N.F. Gamaleya,. 19.7.2) ...
Apart from that, the suppressive action of Helium-Neon laser
light is observed in the multiplication of living cells in a tissue
culture-
Recently, works_have been published concerning the possibility
of obtaining chromosome aberrations under the action of uninterrupte
laser radiation of various wavelengths. He-Ne lasers 6328 A, power
12 milliwatts; argon lasers .4880 A, power 135 mw; nitrogen lasers,
5682 Å, power 1 W, carbon dioxide of 10,600 A, power 1 Watt, were us
to bring out these-effects.Onion roots-of-the-baton-type were sub-
jected to radiation. At the same time, a marked increase of aberrat
output was observed during radiation by spectral lines in the visibl
band. It was discovered that the percentage of the chromosome
aberration output is dependent upon the dosage. . At Wavelength 4880
saturation was observed. With a further-increase of dosage, no-
increase_in_aberration_output was established. For a wavelength-of-
5862 A, the curve was of a resonant character with the maximum
output of chromosome aberrations at 3.4 joules/ cm2.
1
AS is apparent from the material of our review, the majority of
work has been devoted to biological research into the action of
longwave monochromatic-red light of-a-ruby laser of high intensity.
In their work, investigators have drawn attention to research into
changes taking place in fermentations, tissues, etc. occuring during
high-power radiations, when the domonant factor is the thermal
effect. At the same time, hardly any isolated. experimental facts a:
known about gas laser radiations of comparatively low power, but
high-degree of coherence and polarization, possessing non-thermal
specific effects. At the same time, such experimental findings wer
not explained, they were not used as departure points for systemat
research. . Let us now consider a few of. them in greater detail.
E. Karlander and R. .
Kraus .(1968) . did some interesting. ..
experimental work aimed at explaining the physiological effectivene
of light obtained from both coherent and noncoherent sources.
A non-coherent source of light equivalent in energy and wave-
length was a monochromator. One of the indexes of photosynthesis
intensity of chlorella (a plant, which grows in water in closed-typ
ecological systems -- Eds) served as an indication of the degree of
hydrogenation.
The data obtained by the authors is contained in
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UNSPECIFIED-ACTION OF LASER RAYS ON_LIVING ORGANISMS -8.
The authors suggest that coherent light is photosynthetically
more active than non-coherent light of the same wavelength. Laser
light is 20-30 % more effective than that of a white light lamp or
monochrometer. O.P. Sachkov (1969) also observed-a-sharp increase
in the production of hydrogen blue-greenwater plants when they were
subjected to supplementary He-Ne laser light.
Of great interest is the work that has been done by A.I. Semenova
and V.A. Singayevskiy (1969) explaining the biological action of
. low-intensity laser radiation on the living organism (animass) .
A. I. Semenov carried out experiments on 68 white rats. Research was-
made into the hromonal reaction on the suprarenal. Radiation activity
was effected with the aid of He-Ne, ruby, and Na++ lasers. The
.
.
experiments conducted showed that the action of He-Ne laser radiation
of constant activity (i.e. continuous wave or CW --- Eds) over a period
Of 10-20 minutes brings about hormonal reactions in the suprarenal
regions manifesting themselves with the decrease in the number of --
iozynophyles int the peripheral blood by 30-70 %, the increase of
the suprarenal weight by 20-40 %, and the decrase in the content of
lipoids in the suprarenal cortex .. These changes obtain their maximum
value 15-20 minutes after radiation was initiated, and 2-3 hours after
radiation, the hormonal reaction decreased to its initial level.
The return to the initial level took more than 5 or 6 hours-in-
rats with a weak nervous system. A similar hormanal reaction was
hardly noticeable after many rediations with impulse lasers. Apart
from the hormonal reaction a sharp increase in the demand for hydrogen
of 50-loo% was observed. A.I. Semenov shows that a single gas
laser radiation is equivalent to many repeated radiations carried out
with the aid of an OOG of the ruby and Nd+t type.
Furthermore, A. I. Semenov and V.A. Sengayveskiy have discovered-
that by radiating the eyes of animals with even low-intensity gas
laser radiations, wavelength 6328 A, brings about considerable
functional changes in the cardiovascular system. Changes in the tone
of the vessess have been established .. After adaptation to darkness ,
the radiation brings about a marked displacement in arterial-pressure
(on the average-20-30 mm Hg-st. ). Arterial-pressure has a-tendency-
to decrease. _ Sharp changes_in_the_activity_of_hollyesterases take.
place in the direction of its increase after the eyes of a rabbit have
been subjected . to He-Ne radiation .. .. A displacement. of Ca-Na (Calcium
Sodium) balance in the reticular tissue of the eye has been noted.
According to the authors,, all these effects can-bring about changes
in the parasympathetic sector of the nervous system, that is, they
-
are of a vagotropic character.
Consequently, as the various experimental work shows, living
organisms are able to react specifically to the action of laser
radiation of different wavelengths and intensity.
The above-mentioned bibliographical data demonstrates the
-gradual evolution of the opinions of the researchers concerning
the mechanisms of laser light action and the greater interest shown
to the problems of the photobiological non-thermal effects of this
new form of radiation.
To show the perspective of
this development in the utilization
and discovery of the physiological effects of laser radiation one can
-go back as far as 1965 when it was possible to get acquainted with-
the effects of increased biological activity with the aid of mono-
chromatic red light, linearly polarized (V.M. Inyushin, 1965).
Since then, ... 9 years. have elapsed. . As . result of intensive work,
considerable material .concerning the biological activity of mono-
chromatic polarized noncoherent radiation and gas laser radiation has-
been amassed.
The results of these investigations are presented in
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CHAPTER
PHYSIOLOGICAL-ACTION-OF-MONOCHROMATIC-POLARIZED -- RED-
LIGHT ON_PERIPHERAL_BLOOD_AND_BLOOD-FORMING_ORGANS
The first experiments concerning the discovery of the actoon of
laser rays or other Light wave apparatus radiating monochromatic
polarized red light on the composition of peripheral blood and
blood-forming were carried out in Alma-Ata at the faculty-of-biology
of the Kazakh state University,
Such research was neccessary for the evaluation of the specific
reactiveness of the blood-forming organs subjected to light radiation
-of-definite quantum-and-wave parameters. They were to-open the way
for the wide use of laser radiation in the clinic.
During the total radiation of white rats and cats over periods
of a few minutes with noncoherent light of maximum 6300-6400 A
-over-a period-of-lo-days, we-observed-rises-in the content of erito-
cytes and leucocytes (V.M. Inyushin, 1965, 1967). On the 5th day
after radiation the content of hemoglobin increased to more than 12
units. The number of eritrocytes decreased somewhat over the day after
the-initiation-of -the-radiation- and-as-the time went on progressively
increased from 6 million to 8-9 million, towards the loth day of
the experiment.
Similar reactions_were_observed_in_dogs_which were subjected
-to-noncoherent-polarized-monochromatic-red light-radiation-of-the-
very same intensity.
All our experiments were carried out as part of a plan to make
a_comparative_evaluation_of_noncoherent_and_to_a_lesser . degree,
monochromatic-red light-obtained from gas discharge sources and
laser radiation.
Making use of flouresence microscopic studies of
blood smears, we came to the conclusion that under the action of
gas laser radiation wavelength 6328 Å radiation intensity 2 mW/cm-
(without-any-noticable thermal effect) already a day after a double
total radiation of White rats, there was a rapid increase in the
amount of young forms of red blood corpuscles -- reticular sites.
In connection with this and in order to make an accurate evaluation
of bone marrow production or eritrocytes- under the action of stimulants
it is not enough to take only the number of eritrocytes and reticulra-
cytes into consideration, in our laboratory more detailed experiments
were carried out. The average maturation time of reticular sites
was determined according to methods submitted by N.E. Mosyagina (1962).
A series of experiments were
carried out which demonstrated that
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--- 2
4
1.5-2 times, already the 3rd day after the 2 min procedures of
monochromatic polarized red light activity obtained from a
noncoherent source with its maximum in the range of 6400 A and a gas
laser wavelength 632 -A. In addition, the-laser-radiation brings ...
about quite-a-steep-increase of eritropoetic production. But, the
epitropoesis curve of differentiation has a more wave form character
in terms of time then in the case of the action of monochromatic red
light issuing from a noncoherent source. The increased production of
eritrocytes was observed in the case of the activity of total laser ..
radiation. The increased production of eritrocytes in comparison with
the norm was observed also on the 17th day after commencement of
radiation.
Consequently, the effect of monochromatic coherent polarized
light manifests itself as being physiologically a more resonant
Character than the noncoherent, and its effect perpetuates over
longer periods of time after the initial effects. However, the
noncoherent-source-of-polarized-red light_(the apparatus-for-thermal-
light_radiation patent_nr ._ 245995_also_demonstrates a noticeable
effect on the blood-forming organs ....
The condition of the marrow during the radiation. of. living
creatures indicates that this ray factor acts as a stimulator .. It
was also noticed that after a single. 2 min radiation with the aid
of noncoherent_monochromatic polarized red light that the number of
vasophilic_and_polychrome eritroblasts increases on the average by_5-lo"
When repetitive radiations were carried out over a period of
4 days, an
increase of young eritroblasts was observed (bazophil:
polychrome and normoblasts). The number of mielocytes increased,
especially-
the eozinophile by a factor of 3-4-compared to normal-
conditions. These was an increase in the activity of mitotic activity,
of the bone marrow cells.
Also, considerable changes were noticeable in the milt. (spleen) .
In the initial stages, a filling up of the spleen with hemolorized
blood took place, and with further radiation by monochromatic polarized
red light there was an increase in the number of young lymphocytes, --
which demonstrated the existence of-the-activation-of -lymphopoesis .--
) (V.M. Inyushin, 1965, E. P. Smirnov 1967).
To evaluate the reaction response of the spleen we applied the
technique of biotic staining introduced and discovered in their time
by D.N. Nasenov and V.Y. Alexandrov .(1940). The numerical evaluation
of the sorbic stain (neutral red) was carried out with the aid-of-
photoelectro-colorimeter .- The data-show-that-the-maximum-changed-of
sorbical properties is observed from the 3rd to the 6th day after the
commencement of the 2 minute action of monochromatic polarized red
light. The increase of sorbtion compared to the control group
(of animals) comprised from 30-40%. .. During the total radiation by
gas laser light of wavelength 6328 A of adequate duration and intensity
, a-sharp-increase-of-sorbtion-already after the first one or two hours
was observed after radiation. The excess or sorption indicators
(indices) can sometimes be from 1.5-2 times greater than the norm.
At the same time, the consequent drop in the amount of sorbtion staining
takes place more rapidly than in the case of radiation by noncoherent;
monochromatic polarized red light (the data of N.G. Tkachenko. ) .
The results obtained demonstrate that during radiation the
effectiveness of response-is determined by the influence of the degree
of monochromaticity and as far as is possible the coherence of
radiation. During the action of laser light, the response of the
Spleen is more distinctly expressed. It is interesting to comment
that an idiosyncratic accumulation of activity takes place in every
case within the first three days. During the action of. laser light,
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..
. - 3
trained directly on the isolated-spleen, we also observed an increase
in-the-sorbtion -of-the-neutral-red. Consequently,it is possible
to have a direct influence of light on the organ.
The above data demonstrate the real effect of the action of
monochromatic polarized red light,
extracted from the shortwave
section of the red spectrum on the production of blood. Similar
effects are obtained with the aid of laser light. In-both cases, no-
-irreversible pathological-changes of any sort were observed.
In recent times the reaction of blood_forming_organs_were_studied
for the most part in connection with the activity of ionizing radiation
and sometimes ultraviolet light, electromagnetic vibrations of an
ultra-
high and extremely high frequency. The material per present
In this section demonstrates that visible light, especially red light,
exerts-a-stimulating influence on the-composition of-peripheral-blood
and blood=forming_organs ..
Blood-forming reactions in the organisms of living beings
:
-
subjected to long-term action of laser light were studied. In such
experiments we observed a periodic Inhibitive effect on blood forming
which was of a reverse character.
In our opinion, the activity of-
MRL-on-blood-forming organs
and blood_generated by_a laser_of_other_light_sources_takes place
both directly and indirectly.
In the first case, the red light absorbed by the porphirines
can bring about the lessening of the resistance of the older
eritrocytes and their disintegration (photodynamic effect) . This
-process-can be-intensified-by adding_photosensibilitizers-into-the
blood. : The products of disintegration_can_be_activated_by_the blood
forming process of the marrow.
It is known that native blood (A. Chizhevsky 1973) circulating
in the vessel comprises an ordered system. In our laboratory,
I.B. Beklemischev carried out a series of experiments. It-was shown-
that blood comprises-a-system with-labile phases and a high degree
of cooperativeness. The effects of laser radiation can be realized
theough the blood thanks to which an accumulation and migration of
absorbed energy is obtained. During the direct action of OQG
radiation on blood exposed many times, a
"decrease in the
number of absorbed methyline blue-was observed .--- At-the same time,
the character of the sorbtion changes ----- their rise-or fall === were
dependent on the initial indicators. At low initial indications,
radiation as a rule showed their rise,
at high indicators,
their fall.
They suggest that blood is a liquid crystal substance in which
light induces multifarlous energy processes. Consequently, it is
possible-to-assume that-the fact that the action of laser light on
blood is of a direct nature has been proved. Blood is a substance
through which the transfer of absorbed energy to the various points of
the organism takes place. At the same time, the indirect activity
of MPRL on the forming of blood takes place on account of the acti-
vation of the functioning of the endocrinal tissues (glands) , above
all, the hypophysic-(pituitary --- Eds)-and-thyroid-which have a direct
relationship in regulating functions of blood forming
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CHAPTER DY
THE REACTION_OF_ENDOCRINAL_GLANDS_ _ DURING RADIATION
BY MONOCHROMATIC RED LIGHT
The ability of endocrinal glands to react to visible light
Is common knowledge. It has been shown experimentally that under
the influence of visible light, functional and-morphological changes-
which lead to intensified secretion of the gonadotropic hormones in
the blood takes place at the front bottom of the hypophysis. Apart
from that, it is known that the hormones of the front bottom of the.
hypophisis not only control the gonadotropic action but also influence
glands of internal secretion. The function of the thyroid gland is
directly dependent on the intensity of secretion of the tireotrophic-
hormone of the hypophisis. A.A. Voytkevich (1945) experimentally
demonstrated that the tireotrophic function of hypophises in frogs
and toads is activated by the visible light from an incandescent lamp.
There are indications that the hormenal activity of. hypophises is
stimulated by red light (N.A. Popov. 1940, A. Emme 1958,et al).
Not much has been discovered-about-the-biological-effects-of-
separate-monochromatic sectors of visible light. M.E. Zeltser has
made research into the condition of the thyroid glands of white.
rats after they had been subjected to a single total dose of mono-
chromatic red light radiation wavelength. 6400 A over aperiod of
2 and 5 minutes. Already 15 minutes after the introduction of the
-isotope, the amount-of-radioactive iodine-in-the- radiated-rats-was
considerably higher than the control group.
The maximum iodine absorption is observed 2-6 hours after
introduction of the isotope. In that. period, the difference between
the control and experimental groups reached maximum amounts, and .
and was statistically significant ... One day after the .2 min radiation
the condition of the thyroid glands- ` normalized. According to the
author, the results obtained demonstrated the activization of the
thyroid gland_after_a_single_dose_of_monochromatic_red light
radiation. L.S. Prihod'ko and M.E. Zeltser (1967) report of results
obtained on the reaction of suprarena to multiple radiations of
white rays (MPRL) of identical wavelength in experiments to discover
the functional activity of thyroid glands. A decrease in content of
-ascorbic acid-was observed which points to the-hypofunction-of the
superrena. After the single doses of radiation no noticible increases
in abscorbic acid content were observed.
We reached slightly different conclusions while examining the
histophysiology of the superrena. An analysis of the substantive
changes in the superrena subjected-to monochromatic-polarized red
light-and-gas laser light enabled-us to discover a complicated
physiological reaction of this organ. After a single 2 min monochromat
red light dose of radiation, the stain sorbtion usually decreased
which might point to a decrease in function. Towards the 3rd day
after the initiation of double doses of radiation the suprarenal
sorbtion increased about 50%, and towards the 6th day, begins to
-approach the normal level. Laser light brings about a more rapid
increase in sorbtion which is already observable 30 min after_a_single
dose of radiation. Furthermore, a sorbtion increase takes place
reaching an almost 2-fold value with a successive decrease towards
the 6th day and loth day of action.
(Notes: Mitoticic: temporal structure in a dividing cell creating
the movement of-chromosomes towards 5-its poles which insures their
equal distribution between the daughter cells ..
erithrocyte: red blood corpuscle. eritroblast: cell of bone marrow,
precursor of red blood corpuscle. Nucleated, undergoes mitosis, grad-
ually develops hemoglobin. --- Eds)
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.- 2
Consequently,such an-important gland of internal secretion as the
superrenal_happens_to_be_ _ subjected_to_more marked changes in
function under the influence of coherent monochromatic light, 6328 A ,
in comparison with a less monochromatic, noncoherent radiation with its
maximum at about 6400 A.
The stimulation of the superrena is-indicated by biochemical-data
as well-as-results obtained during histological and histochemical
research. The data. prompted the thought that laser light is
utilizible as an anti-inflammatory treatment. At the present time,
our hypothesis has obtained the support inclinics dealing with a
whole series of ailments.
Together with doctor O.A. Zavyalov at the ministry of health
clinical hospital-of-the-Kazakh-SSR,-we- -- observed-70-patients-suffering
from rheumatoid and transformational_distrophic_polyarthritis. of
these, 39 suffered from rheumatoid polyarthritis, and 31 from trans-
formation distrophic polyarthritis. Among those suffering from
rheumatoid polyarthritis, there was a preponderence of women, ages
-35-65 years, the majority, 30, having suffered-for-more-tan-5 years-
They had-many times received treatment in clinics and health resorts
without any noticiable therapeutic effect.
At reception, the sufferers were subjected to a whole complex of
clinical, biochemical, and X-ray examinations, confirming the
diagnosis. According to the degree of activity (according to Nesterov)
and the process phases, the sufferers were calssified as-follows:
-activity of-first-degree (in-15), activity of 2nd- Land- third degree
(in 24), acute exudative phase, in_1 patient, _sub-acute_exudative
proliferative, in 33 and fibrose-sclerotic in 5. patients
The sufferers received treatment from a monochromatic red light
source radiated from a prototype He-Ne laser, LG-75, wavelength
6328 A. Concurrently, 5 patients were given massage and-physio-
therapeutic exercises.
The radiation was of the_general_local_character_of_activity_on
the_damaged joints and reflexo-genic . | zones, applied in physio-
therapy. Radiation exposure varied from 1 to 30 seconds on each area,
and was selected according to the Initial condition of the patient,
the phases and degree of process activity.
-Some patients-experience a slight-vertigo,sleepiness, and
increased-perspiration after the first 2_or_3_sessions ._ After_adaptation
to_radiation, these_symptoms disappeared. After 3-4 sessions, a
photoreaction was observed in all of them. In this period, an increase
in pain in joints was observed (in all the patients) the appearance
of swelling joints, (in 3 patients), the increase in temperature-(-5 .-
"patients). In one patient, a slight increase in arterial pressure was-
-registered. In this period, radiation was prolonged, but with
decreased exposure. In 2-3 days, all the effects dampened down.
Towards the end, the | pain syndrome decreased in 35 patients, the
exudative phenomena died down, the area of joint movement increased,
Morning stiffness Was less marked.
-No marked changes werenoted in the hemogram. The-ROE-became
-slightly more rapid towards the middle of the course, returning to
its original value towards the end of therapy. Towards the middle of
the course, some decrease in lymphocytes took place, levelling out
towards its end. The S -. reactive of white corpuscles did not change
during therapy. The fibroginogen increased towards the loth session,
and decreased towards the 20th, remaining within the norm. The-contnet
of-albumin increased somewhat in the middle of
therapy, and
towards the 20th session decreased to its initial level. Towards the
end of there
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THE REACTION OF ENDOCRINAL GLANDS DURING RADIATION --- 3
Out-of-39 patients, improvements were observed in 35 .. The
polyarthritis and multiple-ancilosis-(bending -ability of-a-joint
to move_as a result of the growth in joint swellings --- Eds) - and- in 1
patient with exudative_proliferative_phase process_who_violated the
regimen. We did not observe any worsening of conditions of negative
changes in the joints of a single patient.
-Milder, delayed results were traced in 21 patients. Of them, 19
with-exudative proliferative changes, a
*rdecrease in-joint paind
was achieved, , an increase in their-mobility, a-decrease-in-morning
stiffness, compared with the_initial condition. In
these patients,
a good therapeutic effect was . obtained after the second session
of treatment. Af first, no improvements were observed in 2 patients,
with fibro-sclerotic process phases.
The dynamics-of -- the -amount-of-leucocytes-and -ROE-in" the second
course of treatment did not change. A tendency-for-the number of
lymphocytes to increase towards the 20th day of the procedures was
observed. Apart from that, a decrease in the content of general
white albumin and alpha-globulins was observed. The S-reactive of
white corpusices did not change.
-In the-group-of transformational distrophic polyarthritis patients
were 17 women and 14 men, aged-between-35 -65-years- with-a-case
history of more than 3 years. Of them 21 with nerve osteo-arthrisis
primary and lo with distrophic damage to joints of different orgin:
changing, hormonal, professional, and others .... Treatment of those
patients was the same as those suffering from rheumatoid
-polyarthritis. In this group, an analagous phtotreaction was observed.
However, the pain syndrome-decreased-more rapidly-than-in -those ---
suffering from rheumatoid_polyarthritis_Also, towards the end of
the course, mobility was increased (area of movement) . There were
improvements of greater or lesser degree in all patients. Hematologica
"data" .reflect the tendency of the ROE to decrease. The number of
-leucocytes increased somewhat towards the middle of the course,
thereafter_decreasing to initial values .- The-number of lymphocytes
increased within normal bounds.
A.M. Moshcheyeva of the ministry of health Kazakh SSR clinical
hospital investigated some biochemical indices of blood during
laser radiation stimulation in 62 patients, suffering from inflammator:
processes-of-the-
> uteral appendages. The dynamics of the white
corpuscle fraction's of-the- blood -serum-was-determined with the
ase .
of A.E. Gurwich's_method .__ The_electrophoresis was carried out- in ___
a buffer medinal -verinal solution of Ph 8.6 with a
_tension
(potential) of 300. V. . The data obtained was quantatively deciphered
on an FEKM (photo-electron microscope ?-- Eds) . Concomitantly, the
quantity of general serum white was determined by the biuretic method,
modified by L.I. Slutskiy (1964)-on-a-spectrophotometer-SF-4A .-
(Note: a biuret reaction is a chemical_test used for.proteins-in
solution. Employs copper sulphate in akaline solution, which gives
pyagle color with proteins and with a few other substances --- Eds) -.
The first group of 31 subjects comprised patients with an acute
form of chronic inflammatory processes taking place in the uteral
appendages. The second group-were patients with inflammatory processes
in the chronic state of illness, 31 subjects.
In the control group of healthy women, the mean level of general
white content was in the region of. 7.90 + 0.77 g & which corresponded
to data found in the literature. In the first group, the content of
general white serum of the blood in 28 patients was within normal"
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The concentration of serum white corpuscles on the average comprised
7.69 ± 0.096 g % which was somewhat below the indices of the control
group. If the indices of the general white corpuscles of blood serum
did not show simular rises, then the separate white corpuscle fraction
-showed-marked-changes .- White corpuscles-were observed-in-only-five ...
patients, while, in 26 of them , a decrease was established. If the
mean index of albumin content in the men of the control_group_comprised
4.68 + 0.042 g %, then in the group with acute chronic inflammatory
processes it was considerably lower: 3.98 ± 0.98 g % at P less than
O.
With the analysis-of globulin indices-of serum blood fractions a-
considerable increase in the content of alpha-2 and gamma-globulins and
less pronounced increases in alpha-l and beta-globulins_was
established. With the determination of the albuno-globulin indatator
coefficient, its decrease was discovered in comparison with the norm,
which points to the sharpening (worsening) of the inflammatory processes
Under-the influence of- laser radiation therapy, tog etherwith
a clinical-improvement genuine changes in the content-of the various-
albumin fractions were observed, which pointed_to_the_cuperation of
the inflammatory processes.
Thus, towards the loth procedure, especially towards the end of
treatment, a consistent Increase in albumin content was observed, the
decrease in-all-globulin fractions, especially the, alpha-2 and
_gamma fractions. The albumin-globin coefficient index attained-a-
characteristic value for healthy women, in practical_terms, 1. 34 + 0.04]
As was expected, the inflammatory process in the chronic stage
of illness presented a different picture. In 20 patients of that
group, the Index of general albumin was somewhat higher than the mean"
index of the control group, and only 1-patient manifested an insignifica
hypoproteinia (6.0 g %). If during aggravation-of-the-inflammatory
process, a decrease of the albumino-globulin coefficient was noticed,
then in the inflammatory process of the chronic state of illness it
was within the normal bounds, 1.56 ± 0.047. Towards the loth procedure,
parallel with the clinical manifestation of the aggravation of the
-inflammatory-process, a certain decrease in albumin content was
observed, the-increase of all globulin-fractions,and-corresponding.
decrease_in_the_albumin-globulin_coefficient_(1. 27 ___ 6.048) ._ Towards
the end of treatment, a normalization of the albumin content of blood
serum manifested itself. Albumins returned more rapidly to normal
and alpha-2 and gamma globulins more slowly. A tendency towards
the normalizations of the albumin coefficient (1.34 + 0.048) was
observed.
In this way, research made into the albumin fractions of blood
serum in the various stages of inflammatory processes of uteral
appendages as well as the influence of laser radiation therapy enabled
the statement of the following principles:
1) The serum protein changes taking place during inflammatory processes
in the uteral appendages reflect-the-
: - acuteness-and severity
of the disease process. The more severe the process, the lower the
quantity of general albumin, the lower the albumin -- globulin index the_
higher the content of globulin.
2) Patients with an acute form of chronic inflammatory processes of
uteral appendages subjected to laser ray therapy parallel with clinical
-recovery, were observed to-reach a -state of-normalization-in-their-
albumin content, general albumin, albumin-globulin coefficient, and
decrease in globulins, especially alpha-2 and beta
fractions.
3) Towards the loth procedure of therapy interferences in the blood
albumin spectrum were observed in the inflammatory process of chronic
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THE REACTION OF ENDOCRINAL GLANDS-DURING RADIATION .. .- 5
earlier rises in albumin fraction_contents.were observed.
Y.E. Bihovskiy of the ministry of health Kazakh SSR clinical
hospital carried out He-Ne laser therapy on 68 ill women, among them
30 suffering-from-acute form of chronic Inflammatory processes. in
the uteral appendages and 38 from-a chronic stage of inflammation.
44 women suffered from_a_two-sided_inflammatory-process, and 24"from"
a 1-sided (i.e. bilateral and unilateral --- Eds). Their-ages-varied-
from 20-43 years.
The duractionof illness varied from 5 months to 17 years. The
cause of illness-in 40 women were the results of_abortions, in 9,
the result of pathological-factors, in 12, the provoking factor was.
chills, in 7, causes could not be determined. . Prior to entering the
clinic, they complained of general deficiencees, lack-of- sleep-and-
-
appetite, irritibility, perspiration , constant or periodic pains in.
-the lower sectors of the stomach, and loins,
with irridations of pain into the lower limbs, painful, abundant
irregular menstrations and-paleness .-
30 patients manifested a sub-fibril-(i.e. below fever --- Eds)
body temperature. 32 women suffered. sterility
All the patients were subjected to clinical_and_sometimes
laboratory tests during the therapy. process. Laser ray therapy was
carried out-according to a method worked out by us .. . Laser light
radiation was carried out-on-a-specially constructed He-Ne laser
LG-75, wavelength_6328_A, intensity_25 mW/cm"
The length of radiation on definite reflexogenic -zones-and
known acupunctural points varied according to the reaction of the
organism to the therapy applfed.
The total-single radiation exposure lasted from lo-15 minutes.
The course of therapy_from-20-25-procedures, which were carried out
daily at exactly the same time.
The patients did not receive medicaments either before-orafter-
subjection to radiation. In 30 women, after the fourth to loth pro --
cedures, the acute stage of chronic inflammatory processes of the
-uteral-appendages-showed an improvement in general condition; a decreas.
in pain below the-stomach-and -. J-loins, normattaation in body
temperature. During this time, a-numberical-increase in the Index
of the sial acid was observed ._ . Although statistically it-turned
out to be insignificant. Analagous results were also obtained by us-
in-the indices of S-reactive albumin content. Towards the end of
therapy -- together with the significant clinical effect, a genuine
decrease in the index-of-the-sial acid was observed in the 25 pati
which pointed_to_the_cuperation of-the inflammatory process. As far
as the S-reactive albumin index is concerned, we did-not-discover any
divergences of its content practically speaking, in any of the healthy
women.
Thus,-after the termination of the first course of therapy of
patients suffering from-acute chronic inflammatory processes in the
uteral appendages, _a_ _ total_therapeutic-effect was attained by 17,
a partial effect by 8, with no effect noted in 5 women .- 13-patients-
who did not obtain the full therapeutic_effect were recommended to
take a repeated course of laser therapy stretching from lo-15sittings.
2-3-months-after the first course .. After the second course ended,
lo out of the-13-patients-attained a full clinical effect, which
was confirmed by the data obtained on the sial acid and the S-reactive
albumin. The effect was absent in '3 patients who were later found to
have a piosalpincs in whose cases operative therapy was neccessary
(Note:from the Greek :pion, and salpincs --- tube, i.e. "rotting tube"
or a limited collection of rot in the uteral tube, which leads to
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Out of 38 patients in the chronic stage of illness, towards the
loth procedure, 22 were observed to undergo a . " worsening of general
condition, and Intensification of pain below the stomach and loins, an
increase in albumin, lack of sleep and appetite, in 8 patients, a
sub-feverish body temperature-appeared .-
1.
All this pointed to the intensification of the inflammatory process
of the uteral appendages. After the first course of treatment 27 out
of the 38 women noticed a disappearance of the inflammatory state
and pains in the regions of the uteral appendages. The latter were
movable at palpation .. 7 patients obtained a partial effect, a certain
decrease in the dimension of inflammatory formation of uteral appendage.
and a decrease in pain at-palpation .. No effect was-noticed-on-4-patient:
When analysing the indices of sfal acid in the process of therapy, a
noticable increase towards the loth procedure was established. In in
some patients, it lasted until the end of the first course of therapy.
(From 0.197 ± 0.09 to 0.214 + 0.016), P less than 0.001) which
-pointedto the aggravation- of-the-inflammatory-process. Towards the
middle of therapy, the S-reactive albumin indicator had a tendency-
to increase somewhat, however, statistically, it turned out to be
insignificant. Il Women in the group which did not obtain a full
effect underwent a repeated course of laser radiation with. good results.
Investigations made into the dynamic. changes of hemograms In
the process of laser light -thepy th
med-that in the process of
treatment, the red blood diagram did not change in either of. the patient
-groups. No significant increases in the quantity of leucocyte contnet
was established. The reactive-index of-eritrocytes-sedimentation ---
(ROE) varied_somewhat_during_the_process of treatment, however_these
changes were within the bounds of physiological norms.
When dealing with the white blood diagram, it is neccessary to .
observe that towards the end of treatment, the quantity of neutrophyles
-definitely decreased in patients with chronic inflammatory processes in
their acute states
In the majority of patients of both groups, together with their
clinical recovery, a certain increase in eozynophyles was observed.
(Note: eosinophil leucocyte: polymorphonuclear leucocyte of vertebrates,
containing granules staining in acid dyes such as eosin. In human
- beings normally about 2-5 % of all leucocytes but become much increased
-in-certain-parasitic-infections,-and-in-allergies --- Eds).
The changes_taking place corresponded to the clinical-recovery
of the patients. We investigated the long-term_results of laser radiatic
therapy in 53 patients over a period of 5-12 months.
Over the period mentioned no symtoms whatsoever
were
observed In any of the women, pointing to the existence of an inflammator
process which is confirmed by clinical investigations of data.
Z women who earlier suffered from sterility, became pregnant.
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Chapter V . Stimulation of regeneration by monochromatic
red-light.
By the local action of polarized MPRL (monochromatic polarized red
light) wavelength from 6-300-6400-A on-skin wounds (lesions) distribute:
on the ear of a rabbit, a characteristic histologic_change_was
observed which pointed. to the stimulation of regenerative processes.
This was done by B. L.Koritniy and collaborators in 1967. Although the
experiments were carried out on the same rabbit, one ear being
radiated by monochromatic polarixed tighet and the other serving
-purposes of control, distinct morphological-changes manifested-
themselves under the action of red light. And so, in the wound
which was subjected to radiation, already within a period of three
hours, a. larger number of neutrophyles began to appear than in
the control ear. In succussive periods, an increase in the in-
tensity of phagocytis was i ted. The bacterial flora compesed
of-diplo-and-tetra-cocci-almost-entirely phagocytosized-in-the
wound exudate. A more rapid healing of wounds was_registered_when
monochromatic polarized red light radiation was applied. At the
basis of the observ'ed: effect, according to the authors, lies the
activation of the transformation of properties in the skin during
the local activity of polarized red light.
A large-cycle of-work was-earried-out-by-prof -. D.L.Koritniy,
explaining:some of_the_morphological.and histological_displacements
in the_auto-transplantation of the skin of a rabbit during the action
cf monochromatic red light: It is curious that during macroscpic
ciservations, a very distinct difference in the condition of
transplanted skin (shreds) Is engendered with-hardly noticeable
chitterlings-(tripe ;- intenstines) -- During histological-trials
characteristic_ hanges manifested_themselves_already_in_the_course
the_first days after radiation was initiated. The : leucoctytal
ill in the radiated skin shreds was considerably more prominent
h comparison with those that were not radiated. During the activity"
{ achochromatic polarized red light an accelaration of inflammatory
process- phase-changes occur, and the proliferation of fibroblastic
intents is stimulated. Characteristic is the absence of fibrose
ichments (nutrients) which in unradiated auto-transplants
a;fear as whole . separate areas.
he same time, on the. background of the stimulatoon of connective
c'elements, the re-epidermation of the radiated transplant
down :- Onlytowards-the-twentieth-day does the whole transplant
:come-covered bythe epidermis created. In radiated_transplants
wronts of the epithelia along theceondective tissue slows down,
son with the control samples, for example by ten days.
in the restituted: phase a new epithelial covering of the
transplant manifests a quickening tempo of its productivity.
tod-of-one month after the transplantation the organo-
structure of the radiated transplant :- reestablishes
c the same degree. as transplants not subjected: to the activity
romatic polarized red light do in a period. of. 2-3 months.
the analysis of. glycogenes in the epidermis, it. has been
' that under the Influence of monochromatic polarized red light
ertod of ten days. (lex24 hrs. ) an increase in concentration
Astor-this, a decrease of glycogene concentration in the
cf skin_shreds takes place, when compared with control
ich is explicible by the more greatly accelerated tempos
:> differentiation.
:3"CY RAK change reflects the entire regenerative process-
Wepresence of auto-transplantation. In the first lo days
setsration, the concentration of UNK in the radiated sample
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Stimulation of regeneration by monochromatic red light. - 2
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exceeds_this_in_ the_ control_sample, in_this_case_twofold.
according to D. L. Koritniy, the RNK concentration in the epithelia
of the radiated transplants is relatively lower on the 20th day and
is even ower on the 30th day after transplantation, this being
explained y the increase-of the morphological processes in the
epithelial-plast. It follows that-monochromatic-polarized-red light
turns out to be a powerful stimulator of regenerative processes in
wounds as well as in freely transplanted skin.
The next experiments were carried out using coherent red light. In
the republic clinical hospital of the ministry of health of the
Kazakh soviet socialist republic in the biophysical laboratory of-
the Kazakh university and-in other medical-biological establishments.
a considerable amount of factual material has been collected demon =_
stating the possibility of utilizing the biostimulation of physiologi-
sal processes with the aid of monochromatic light radiations
from
He-Ne lasers. Some therapeutical aspects of the effectiveness of
such biostimulation were learned.
Below, we reveal our-experience during-experimental work concerned
with the stimulation of regenerating_processes_ by_the_radiation of
He-Ne lasers and our clinical work when we used low-power lasers for
the treatment of some ailments ..
The rapidity of the process of regeneration, its stimulation, or
inhibition is a good indicator of the activity of chemical or physical
agents
Assuming that the endogenal ray regimen of mitogenetic radiation
during regenerative processes manifests itself as one of the con-
ditions determining the proliferation of cells, we carried out a
series of expeiments to explain the possibility of stimulating
regenerations by laser light on account-of-the-induction of-secondary
radiation in-the UV waveband The possible aspects of applying
low-intensity_He-Ne_laser_light_for_the_regeneration of bone tissue
were learned. The experiments were carried out by. P. P. Chekyrov on
72 mongrels. The regeneration was studied on the radius bone when it
was sawn. (partal .cleavage) and. total cross-section fracture. According
to bibliographical data during the trauma of the radius-bone, -its --
regeneration takes place according to the first mentioned type. At
total cross section fraction of the radius bone the second type of
regeneration predominates, with theappearance of bone overgrowth
(bones. becomes thicker) . The operations Were carried out according to
strictly standard methods in aseptical conditions under general
narcosis. The depth of. the surface sawing was measured with the aid-
of-dividers :- The wound -- was sewn-up-hermetically after which an anti-
septic bandage was placed: To attain the total fracture of the
radius bone, an electrical cutter was used, the cut made was equel
to 4/5 ths of the. bone. diameter, after which it was broken with
the aid of mechanical force. The remaining operations were the same
as in the first case ..
-On the plaster-of-paris-binding, at the site of the fracture an
opening_1 cm sq in diameter, was made, to facilitate radiation by
laser light. The locality of the fracture was radiated with coherent
monochromatic light of the optical quantum generator .. wavelength 6328 Å
of intensity lo mw/cm. ( . Diagram. 3 - caption: external view of He-Ne
laser for the purpose of radiation bone fractures and wounds).
Radiation was carried out in conditions of darkness, without additiona.
illumination, Exposures varied from 1-lo m nutes. Observations were
made in periods of 60-90 days after the trauma. was made. In those
periods, all dogs underwent. roentenography of the radius bone,
comparing the. roentgenograms. obtained with the control samples
made during the same period of observation, the following was observed
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Stimulation of regeneration by monochromatic red light. - 3-
On the- X-ray of-the-control_dog, the saw cutting_was still_observed_
at all stages but showed small contrasts. The edge of the bone opposite
the cutting, an area of. peri-remnant bone thickening was formed.
This peri-remnant covering. has its greatest. density on the circumferenc
Without having to go into detailled discriptions . of other experiment
it is possible to summarize the results-of-the-experiments. The degree
of healing of the-bone.thickness in the experimental dogs was considera
ly higher than in the control dogs. Thus, for example, the first_signs
of development of bone thickening (growth) in the control dogs appearec.
towards the 20th day, whereas in the experimental. dogs, towards the
loth day. On the 20th day of observation, the bone overgrowth In the
experimental dogs appeared in theform of a protuberance on the opposite
side of-the-surface-sawing-of- the radius-bone. The_sector_of the-radius
bone in the area of sawing was compacted (filled in). Towards the 60th
day in dogs of the series. mentioned, the effect was already unopserv-
able, whereas in the control dogs, it persisted at the edge of the
radius bone.
"By comparing the x-rays of dogs-of-both series-on-the-90th-day,-it --
-was established that-in-the-experimental dogs, a total regeneration __
of the structure of the radius bone took place, in the control group,
the localitu of the sawing was visible in the form of a thickening
Of that part of the radius bone. Histological. experiments also
point to the indubitable effectiveness of stimulaing lasers-on bone-
regeneration.
What is the mechanism of such action? Does not warmth influence
the rapidity of the regenerative processes? ( Diagram 4: X-rays of
the radius bone of dogs, 15th day after fracture: A .. control group,
B .. stimulation by laser radiation) .
Putting the question in this way. is apparently. insolvable, since
thermal effect cannot contribute its healing effect. to biological
-activity in connection withthe fact that hardly-any-heating-of-
the tissue during radiation takes place. For example, during an
exposure of ten minutes, at radiative piwer lo-12 mW/cm_sq the local
heating of the tissue all-in-all comprises from o, 5-1. degree centigrade
Such a thermal. heating can hardly bring about such manifest physiologi-
cal and morphological. changes "the regenerating area. At the same -ti-
one should not-totally-refute-that-thermal-phenomena-do -- not-manifest-
a certain influence on_capillary -_ trophic surfaces . . However, such
activity is not. so. manifest. as to influence the speed of regenerating
processes so that one could be in a state to observe them in our
experiments.
It is known that warm baths, and suchlike, heat tissue. by-5-10 degrees
E-and-more. By increasing-the temperature of-radiation, acting on
-
the contrary an inhibition of the regenerative processes takes place.
At the same time, during. the action of. laser radiation. of. low intensity
and exposure, the increase in temperature. is several times lower.
On the other hand, during the same intensities of the acting factor,
no distinct stimulating. effects. in the dark-red part-of-the spectrum-
-are observable, which confirms. the-frequency-dependant.
character of the activity of the radiating_agent, in particular_the
He-Ne laser of wavelength 6328 . A. This demonstates - the resonance .
mechanism of action of the given factor.
We suggest that the basic matrix resonting on the action of
menochromatic, coherent radiation, is the bioplasma. However, before
going on to consideration of energy changes in bioplasma subjected
to the activity of radiation, it would be expedient to review a whole
series of other clinical and experimental. data, demonstrating the
non-thermal character and at the same time the- highly stimulating
action of He-Ne - laser radiation.
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Stimulation of regeneration by monochromatic red light. - 44
Numerous observations made in our clinic and other medical
establishments point to the possibility of beneficial action of
He-Ne laser light on the process-of-the-post-traumatic-regeneration
of skin ._ " Initially, such data in the experiments were obtained
by D. L. Koritney during the action of non-coherent; partly-polarized.
red light on the skin auto-transplant of a rabbit, of which we spoke
previously . During . very small intensities of from 0.2-0.5 mW/cm- a
distinct stimulating effect was obtained, at which It was observed
that the first to react to-the action of light were the regenerating
processes in the connective tissue.
-
Clinical experiments were carried out to discover possibilities
of. utili zing. He-Ne laser light for puposes of resonance stimulation
of regeneration rrocesses in cases of trophic, boils of various
ætiology. Therapy of five patients suffering from trophic boils of
ray etiology was carried out-by the researcher-from,-Alma-Ata,
K.D. Durmanov. The legth of ailment varied from 1-9 years. the-age-
of patients varied from 23-67 years. Radiation boils were located
in the area of the torso in two patients, the face in one, on the
knee in one and the sole of the foot in one. The diameter of the
boils for sores) was from. 2-5 cm. Exposure at Intensity. 25 mW/cm2
was 1.5 minutes. The patients underwent daily radiation, over a period
of 20 days. In all cases, positive therapeutic effects were obtained,
expresssing. themselves in the total healing of the sores. In_the
republic. clinical hospital . analogical results were. obtained when
laser radiation was used for curing. protracted unhealing trophic
cores. Of interest are.experiments made by V.V. Makeyeva and
collaborators, 1972-to-discover-the possibility-of-utilizing-
He-Ne lasers in the treatment of trophic sores ._ In_13 patients,
the wounds appeared as the result of operative intervention,
in four, as a result of traumas and burns, trophic sores as a result
of thromboflibitis were noted in 8 patients. The maximum area of the
sores was equal to 27 cm sq. All the patients had previously under-
gone conventional,-and-some of-their number, operative treatment.
The length of the ailments_ranged from 1 month to 25 years.
The action of He-Ne laser on the area. of the wound was carried out
daily with an exposure lasting from 20-30 seconds, with its gradual
increase.
-
As a result of therapy, 19 patients after 25 sestoms, achieved-
-total healing-of- wounds-with che-appearance-ef-uniform-tissue-chit ---
terling and epithelization; in 4 patients a considerable reduction in
the dimension of wounds appeared: Already after from 3-5 sesions of
radiation of was observed. in. the majority of patients. a. growth of
granulations; the considerable betterment of their condition, the
disappearance of pain in the area of the wound and normalization of
sleep. as an example, we give here an extract of the case of -a
sickness ;:
A 35 yr old patient"G" entered the traumatological ward on the 1st
Oct 1970 after sericusdamage. to the soft tissues of. the sole and the
large toe of the right foot: In the area indicated was a lacerated
bruised wound with the formation of. cutaneal. shred on the sole.
A surgical transformation of the woundwas carried out. by sewing the
-shred of the skin to its place. As a result, a total necrosis of the
shred took place with the appearance of a granulated wound.
On the 21st of Oct 1970, an operation was carried out to cover the
defect of the skin with auto-transplant, which ended within two weeks
With the almost total ripping. off of the previous transplant.
On 26 Nov 1970, the treatment of the wound by laser radiation was begur.
Objectively: the wound of the sole area of the large toe is in the
shape of rhombus, the area was 13, 9 cm sq, the depth was 2-3 mm. At
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1
Stimulation_of_regeneration_by_monochromatic_red_light .__ 5
At the bottom of the wound, separate eyelets of granulated tissue were
seen, in the form of droplets. The wound was bordered by tripe tissues
(lacerated)
: the-separations-were -- insuficier.
(por), the periphery_of_the wound_secreting_as_a_result_of selling,
were infiltrated and slightly torn op (lacerated) , and at palpation,
painful ..
Summary:Two courses of treatment were carried out. The first of
27 sessions and after a lo days interval,a second, of-16-sessions.
After the first session of radiating the surface-of-the-wound-with-
diffusely focused monochromatic red laser light,_a_zone_of_ epithelial
appeared on the internal edge of the wound and a bright granulated
tissue appeared. In three weeks, the area of the wound decreased to
5.3 cm sq. That is, that the area of the wound decreased by 2.9%
daily.
The condition of-the-patient is-good, .- There -are-no-complaints.
After_the_second_course_of_therapy_the_wound_was_ totally.
epithelialized. We observe a distinctly expressed stimulating
effect. during treatment of trophic boils (ulcers, abcesses)
of radiation etiology. As an example we present a case history
of the illness.
The patient G, 28-years of-age-entered the long-term-treatment
clinic_on_Feb_1, 1968_with_the_diagnosis_of_advanced_X-ray
ulcer of the right heel. After a completed course of X-ray
therapy in Oct. 1965, due to a wart, an ulcer appeared.
Ointments and physiotherapy . turned out to be of no effect.
The course-of. therapy carried out-in-Karananda-and - Moscow
also did-not give any-results. After-entering_long-term. treat-
ment,_the_patient_had_anabcess_measuring_3.5_cm_ with_ a_smooth
bottom on his right heel. It was round in shape , with a
depth of about. 4 mm. There were no perifocal reactions. The
bottom was covered by meager (poor) rotting secretion of
withered granulation. After laser -ray therapy was- carried out
-on-the-right-heel in the Area of abcess localization compact
dense_fluting_(scarring). in height_not_exceeding_the surrounding
sectors, was formed. The surace was smooth, there was scanty
husking, the edges were well continued. . No pain. was noted. The
result of the therapy carried out Was evaluated as being
excellent:
The patient-was once-more-examined, four years after_therapy.
He feels well and no. relapse was observable.
F
In this way, the above mentioned. facts demonstrate that short-wave
coherent red light is capable of recreating. the regeneration processes
on the merit of Its biotic character, the character of Its resonating
-action. In the burn ward of the republick-clinical-hospital-of-the-
-ministry of health of Kazakh SSR, I.T.Kovinskiy, et al successfully
applied laser light of low intensity for the stimulation of reviving ski
grafts and. the regeneration of. wounds resulting from serious burns.
Radiation was carried out for one. day with the aid Of. a. He-Ne laser
type OQG 12, wavelength 6328. A. All In all, 160 patients. underwent -
IRegapy . .
In. their number. (among them>40-had-second - to-third
-- degree burns, 60. had-3rd - degree-B-to-4th degree. As a result of thera
a decrease in the healing period of burns was observed in from 40-50%
of cases. The granulations are bright red, juicy, and tend to bleed
easily. After 5-lo radiations, a violent increase of epithelia is
"0
teucacyte .
observable. At the same time, the phagocytic activity
activates Itselfwhich/000108:07+ CIA RDR96-007&78000500030001-5
eneration-
in Approved serious burn, Apart from that, it was observed that the radi
ted auto-transplant revives more quickly. Extreme epithelia is accelors-
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STIMULATION OF REGENERATION BY MRL. .
.- 6
-ated, blood-supply_is_considerably better, and the growth-of
vascular networks takes place considerably more rapidly.
Histological experiments have. shown that. in radiated auto-
transplants. (grafts) a.more.active morphological. change of
structures takes place. As a result of the action of laser
radiation, the-oxygenation processes are enhanced. This reflects
the magnified metabolism in the regenerating tissues, probably.on
account of the activation of oxygen in some_cytochrome.
One: should not: fail to dwell. upon the experiments on the
stimulation. of. nerve regeneration. during. the action. of laser light of
low intensities, since they help us to understand the mechanism of
the influence of laser light on the regenerative processes.
Considerable amount_of_work on this problem has been done-at-
the Alma-Ata medical institute in the laboratory of Po Prof. A.R.
Rahishev and his collaborator . V. P. Tsoy , and in the electrophysiol-
ogical section of. the institutes of , local pathology of the ministry
of health of the Kazakh SSR, by P. Boyko and O.M. Ratzbam. In an
experiment, -.. . V.P. Tsoy- showed_that- during a. the action of low-
intensity laser radiation on a damaged isciatic nerve, a considerable
acceleration in regeneration of electrophysiological functions is
observed.
The. data. obtained. demonstrate the specific. stimulating action
of He-Ne laser light on the regenerative processes. in the nervous
-system.
In accordance with the experiments_of_O.M. Ratzbaum and
z. P. Boyko, during. low-intensity laser light beamed_on the neuro-
muscular. sample, . a series . of: functional changes. take place in it.
The
authors. suggest h that He-Ne laser light of an initial power of
0.5 mW/cm" . basically acts during the first anelectronic phase of
parabiesis
We are in possession of clinical observations made on 9
patients suffering from_spinal cord ailments (myelitis of different
localization) .. . Dr. N.I. Paremskiy attempted. to utilize the He-Ne
laser. rays. for. the purpose.of. biostimulation of regenerative
processes, as well as a factor against inflammation .. 5-out of the
-9-patients-suffered from-arachnomyelitis of an infectious origin; - 4-
whose illness was- of a traumatic origin. There were 7 men and 2
women. The ages of the patients during the period of_treatment were (>
up to. 20. years of age., 1,. from 30-40, 2, from 40-50, 5, from 50-60,
1. The patients were invalids. The first group. . . comprised. 5 people2
the 2nd 3, the third group-1 ...
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-In-four of the patients, the illness-progressed, -with an increase
in temperature and other general symptoms of infection: headache,
incapacity. (indisposition), cararch of the upper side of the
breathing .channels. .. In. the. majority. of. cases, . the increase in spinal
symptoms. was. observed .. In.4.casesy: of.myelitis. of traumatic origin,
a pain syndrome. was predominant .. The. a pains were. localized in the"
-
-region of-the waist, leins, stomach, ami- - along-the ischial-nerve.
There intensity varied from being of a light, constant-character,-to-
sharp, shooting or searing; pains,
In the great majority of cases, . there were: leg. ailments, weakness
of the hands .was. only. observed in 2 patients .. In. 5 patients, paralysis
and partial. paralysis were of a spastic character, accompanied by
hypotonie-and high tendinous (sinious) reflexes .. Paralysis and
partial parlysis of a peripheral character with atrophy and hypertonic
of the muscles , withering or absence of tendinous reflexes, in 3
patients.
In all cases, disarrangement of various degrees of palpability
was discovered :. The disturbance
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CHAPTER VII A WORD ABOUT BIOPLASMA
It was_said by Biruni, the great wise_man_and_encyclopædist
of central asia : "the body of man is as light unto the world. "
In the east about a thousand years ago the idea was founded about
the unity of the world. Man is not distinct from the universe, but-
-is its-microcopy. And so, we see-already-in-ancient-times-the-
conception existed_of_a_convergence_between_the microcosmos, man,
and the macrocosmos --- the universe. Thousands of years went by
before the philosophical idea of the unity of the world took on a
physical meaning. And here, already in our twentieth century you
begin to truly wonder at the insight of the ancients .-
In reality, the organism is built up of atoms and molecultes
according to the meendelevian tables of chemical elements. Classically
we find that the living substratum consists of substances or elements
in liquid, gaseous and solid states. But could there also be a
state of plasma in a living organism? We are accustomed to represent-
ing any object as being composed of atoms and molecules, but an object
can also be represented as being composed of elementary particles
or ions (plasma). Plasma is the fourth state of matter. It is
at present_possible_to_state_with_confidence_that_nearly all_matter
in the universe manifests itself as plasma. The macrocosmos is
filled with plasma. Is the microcosmos therefore deprived of ft?
Does a plasma of a living Organism exist? Some kind of special
plasma, Bioplasma:
If the world is material and unified then one is obviously
made to say ,yes! In fact, this state of matter -- this plasma -- is
the most widespread in the universe surrounding us. Therefore, it
should also be manifest in the living substratum. Perhaps because
we do not know much about this living plasma, we are ignorant of
the conditions neccessary for creating life. In short, a number
of questions, presently unanswerable if one is confined to present
theories of certain experimental facts, can be looked at in a new
way concurrently with the planning of new experiments, different in
principle from those previously carried out.
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In 1967 we made the acquaintance of engineer v.S. Grieschenko
who presented the hypothesis of bioplasma. In accordance with his-
-presentation ,bioplasma was made up of hypothetical atoms of IKS
Although we became_fond_of_Grieschenko's hypothesis, at the time there
were no concrete experimental confirmations. Later, when studying
the literature, we discovered that the hypothesis of a fourth state
of matter embracing living organisms was also advanced by the
famous english physicist W. Crookes.
-The-science-of-polarized-physiological-energy was developed by
the russian scientist M.V. Pogorelski in 1912 in his book entitled:
"Electrophosphenes and energo-graphics." He noticed that photographs
obtained in gas discharges reflected some kind of physiological states
in living organisms and man: "All natural bodies and all living
matter possess a known number of-physiological-energies which they
constantly emanate from themselves. Exactly as all physical forces __
-known_to_us like gravitation, electromagnetism (heat, light , chemical
reactions) , can also serve as sources generating this energy as well. . '
(Note: certain passages in this section , corresponding to pp50-51
in the original manuscript, have been omitted as being redundant -- Eds)
Academician E. Markowski, director of the biochemical institute -
in Bucharest who-was in Alma-ata-for-a-visit,-discussed-with us-the-
conceptual-state of affairs of the science of bioplasma. Ever since .
the_40's Markowski has been developing models of living tissues
being composed of very labile biostructures, organic clusters and
water molecules, which make up the cells and membranes. After carrying
oLt many experiments, he formed the science of biostruct
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BIOPLASMA -2
whose premises converge with the concept of biplasma. Not long ago,
he sent his large monograph entitled "the structure of living matter"
(Bucharest) in which he writes about the convergence of the concept
of bioplasma with his biostructures concept
The Polish investigator V. Sedlak in a work entitled "A model
of a system emitting bi logical and electrostatic fields" published
in 1967 in the journal Cosmos,
came-to-the conclusion-that-research- -
Into living plasma was neccessary.
In a series of other works, he
has advanced an intrinsic hypothesis of bioplasma (+). According
to his suggestion, bioplasma has_very similar_properties_to_semicon-
ductor-plasma
This has been a short review of the creation of the bioplasma
hypothesis, that bioplasma is an unstable structure of living
.organisms
(+)-See Sedlaks-paper-in-Proceedings-of-the-2nd-International-Congress
on Psychotronics Research, Monte Carlo, 1975, pp.
=== Eds
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we shall acquaint ourselves with the most important biological
properties of plasma, as plasma is obtained usually on our planet.
-The easiest way of forming plasma is to set up an tosizing
gas discharge_During discharge,-an-electron-ion-avalanche-is - formed,
and a gas discharge plasma_appears between the electrodes. The
number of positively and negatively charged particles is equal. As
a whole, plasma is electrically neutral.
Various kinds of vibrations
can take place depending on the particle density. One of the prop-
erties of plasma is the collective character of processes taking place
in part of the plasma. Plasma-behaves as one-whole.
With the help of magnetic fields the stabilization of plasma-can-be
obtained. As far as energy radiation is concerned, plasma is_an
unstable medium. The discharge temperature of plasma is very high --
up to 1 million degrees. The problem of creating maximal stability of
gas discharge plasma is a basic task of modern physics.
Having read the above lines-,some readers-may-begin to have-doubts :
is there hope among biologists in proving the existence of plasma
in the -' soft' energy conditions of living organisms? How instable is
the plasma? Do not hurry to join the sceptics since physics already
has some data allowing the rejection of these doubts. It has been
-shown that under certain conditions, such as in atomic-molecular
-lattices, plasma-conditions-(objects-with-absolute-instability)-can-
exist. In solid-state physics, research has already been done on
plasma effects in semiconductors, crystals and other matter.
The most characteristic thing about the solid-state plasma --
in distinction to its gaseous form -- is Its instability, thermal
-equilibrium, and parametric differences depending on the state of
the-matter. In a-solid body,-electron-hole, exiton,- and electron-
proton_plasmas_are_observable_The hole_ is_a_vacancy of the electron-
in a semiconductor. In contrast to the negative electron, a hole
has a positive charge. A combination of a hole and an electron
gives a neutral particle: the exiton.
The density of the electron-hole plasma in a semiconductor changes
.- according to its-temperature .- During-heating,- the- population - of - free
-electrons_ and_holes_are significantly_increased., ,The density.
2
of solid-state plasma is optimally high: from lot2-lo
"_ electrons/cm
Consequently, the frequency of the plasma approaches the ultraviolet
and visible portions of the spectrum. Each electron or hole does
"not belong to one atom only, but has broken free of the crystal
lattice of-the-solid-body-and-manifests- - itself as a part of
the-ensemble of_the_plasmic_semiconductor. In fact, physicists. have
discovered a large class of convergences -= organic semiconductors
which are extremely close in structure to the structure of living
organisms. Plasma conditions have also been discovered in organic
semiconductors. Therefore, seen in perspective, there is a hope that
a bridge can be built extending from the plasma-of inorganic-
substances to the plasma of living organisms.
Further,a search of the biological literature_shows that at present
there is a considerable amount of information about the existence
of free electrons and protons in biological objects. By the word
"free" is understood an electron or proton which does not belong
to-a specific atom. That is, its position extends beyond-the-
·attraction radius of the atomic-forces
In 1967 St. Dierdi drew attention to the important role of
delocalized electrons in biological processes. It was he who raised
the question of research into semiconductor properties of biological
Molecules (white corpuscles,.
RNA, albumin, etc./) Many
researchers point to semiconductor properties of DNA. The so-called
?_~ electrons play a large part. In biological systems, for example
attochondria (respiratory cell organelles), electron transfer takes place
brauch asmall chat
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A WORD ABOUT BIOPLASMA
-4
through a small chain of respiratory fermentations. The role of :
mitochondria is very large in the process of energy conversion.
Their number can for example be To which comprise about It of the
weight of a human being. They create-energy-reserves in the cell on
account_of_energy conversion_emitted_during biological-oxidation-into --
potential energy of rich chemical energy combinations. According to the
opinion of the Polish researcher S. Monczarski in mitochondria are found
electron plasmas with greater density than in the fonosphere.
Research made into the electrical properties of cell membranes :
carried on quite recently by L.A. Pirozyan and V.N. Aristarhov have
shown the existence of semiconductivity in membranes. The role of
membranes in biological processes is enormous. One can say that nearly
all of these processes act thanks to the membrane. This, according
to how many of them are found in one cell, is manifested by the electron
photograph (illustration b) made by us. Namely, membranes as we shall see
later also appear-as reservoirs-where electron-hole exiton plasma is -
concealed (stored),
The plasma is formed on account of the ionization and formation of
the localized charged particles. However, the converse process also
takes place: the interaction of particles between themselves and their
return to their binding with atoms. This process is accompanyled by
-the-radiation-of-energy-in-the-form-of-separate intensive , quanta.
Bio-luminescence_of_tissue and cells are_at_present-intensively-
studied by the school of Prof. B. N. Tarusov, the chair of biophysics,
Moscow state University. It has been established. that during the
exchange reaction process, the cells luminesce in the visible part of
the spectrum. These are very weak-intensity streams of light which
can hardly be magnified by highly sensitive-light-receptors-(detectors)
sc=called photoelectron magnifiers. As mentioned previously, as far
back as 1923 Prof. A.G. Gurvich pointed to the presence of radiation
in the ultraviolet portion of the spectrum during cell splitting
(mitosis) or during the death of cells.
Discharges of light during
cell death demonstrate the saturation ofrbiostructural energy in the
presence-of-charged-particles.
In this way no mean amount of-data_(experimental-facts) has been-
collected to this day. This indicates_convincingly_that_ free electrons"
and protons play a great role in the vital processes or organisms.
We suggest that bioplasma presented as being electron-hole and
AXEton plasma which localizes itself in membrane structures, among othe:
in mitochondria. This is the most labile view of-plasma in the
ilving organism. Apart from that, a more stable plasma is represented-
as being electron-proton. It is localized in the cell nucleus, the
bicstructural parts of the cytoplasm where no membranes are in
evidence. This is visually illustrated on a simplified bioplasma
model (illustration 6) showing a cell.
What distinguishes bioplasma from ordinary semiconductive plasma?
Bicplasma_is a thermodynamically ; non-equilibrium system-but-which
possesses a large degree of stability in the scope of the conditions of
living organisms. Its most inalienable characteristic is its
anti-entropism.
Further, bioplasma is an organized system. Within itself it does
not have thermal "noise". It follows that it is possible to speak of
-the-bioplasma being a plasma at absolute zero. Of course, absolute
zero is not brought about on account of the decrease or lowering of
kinetic energy making up particles, but conversely, by a "knot of
particles" put together by lines of a force field RenateBring them.
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-A-WORD ABOUT BIOPLASMA --- 5
"binding" of them by electromagnetic fields of biomolecules, ---
all this creates the basis of the stability of bioplasma during
absence of thermal-vibration and-the direction of large reserves-of-
particle energy. Absolutely, bioplasma is made up of oxygen-ions,
carbonic acids, and so on. It is possible to draw_an_analogy_between
bioplasma and ion-reinforced constructions where the manifoldness of
material is made up of durable / : solid) building material.
And so, the most Important characteristic of bioplasma is its
orderliness, its-unusual stability during the time when it is
saturated with energy
Bioplasma is a medium in which different kinds of electromagnetic
acoustic, and possibly gravitational waves are distributed. The wave
characteristics of Vibration is such a medium are spread in definite
cross-sections (planes) and can be polarized. There are reasons to"
-
state that it is characteristic for these vibrations-to-have a high --
degree of coherence, that is, their-phase-characteristics-very strict
coincide. Namely, a wave field has been "frozen_in" as if with
precise wave characteristics. It is as if a unique organismal hologr
has been created. Every fragment of such a hologram possesses a
characteristic of the most essential properties of the whole organi
It is not true to say that being -uncovered-here-are completely new --
approaches to the learning about the memory of the brain, or-the
nature of heridity.
The hologram "frozen in" the bioplasma is in fact a biofield.
That is why we believe that the bloplasma is a medium in which it is
possible to form the basics of the biofield and its fundaments. In
.keeping with-our-conception biofields are a synthesis of real-physical
fields with definite physical parameters and configurations. - The
conservation of parameters and configurations of_fields_is_conditione
by the bioplasma not possessing thermal noise ..
In fact, presentations about the blofield are not new, and have
"their own history. However, at the time"A.G. Gurwich developed
-scientific presentations about the-biofield, they did not meet with-
understanding . - on the part of scientists, and hardly any speculation
on_the_significance of this problem was made.
The question arises: what is the nature of the biofield? How
far does it extend within the living organism?
We believe that the matrix of the field is represented by the
bioplasma. Namely the bioplasmal body does generate-anisotropic field
.Anisotropy is an inalienable_property .of-the-biofield.The -- problem-of
interactions at a distance stands_ sharply and_ to_this day, even tho
proofs
of its existence in man have been obtained, as well as in ma
mammels and even in plants.
Let us now pause to look at some very interesting experiments of
the American scientist K. - Backster. He delved into the changes of
electrical characteristics in plants during many fluctuations of the
biofield. Such fluctuations, as the scientist rightly . "> suggested,
can occur for example during rapid death of the plant or animal, carri
out in the absence of a human being. ( )
() (Note: This Section has been omitted since Backster's experiments
are available in English. See, for example Int. Jn Parapsychology,
Nr .- 4, 1968 and-Science, Vol , Nr. - PP. , 1975 -- Eds)
Independently of Backster's experiments, together with A.S. Romer
in 1968 it was concluded that plants react to changes of fields in the
human being especially during various autosuggestive states
of
Effected character.
The effect was observed at a distance of up to 1 meter, and so,
there exists a basis
-that the bioplasma radiates-ar
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A WORD ABOUT BIOPLASMA -6
We first encountered the bioplasma during research made Into
-living-objects during-gas discharges with-the-aid of the-method
presented with the assistance of Kirlian (V.M. Inyushin 1966-67).
As the research of V.G. Adamenko has shown the imaging of different
objects with the aid of high-frequency currents were made with the
aid of the phenomenon of cold electron emission.
"Not only does the electron emission-accompany-a-given process,-il
fact, other-particles-such as-protons participate in it (see illustra:
Nr. 7). (Caption: Corona incandesence of human finger. )
If we thought of one of theemitters of electrons and other parti
les as being a sector of a living object then in accordance with
changes in intensity of emission it is possible to establish (postulat
although obliquely, the electrical condition of a living-object-which-
-is-connected-with-physical-processes-
In our laboratory_a. . special_apparatus_was_created_to_measure
the intensity of light radiation taking place in a discharge envelope.
During research m-ade into plants we discovered that the intensity of
radiation is at its maximum in young leaves, and at a minimum in old o:
During the process of the research made-at diminuation of-the intensit:
-of-radiation was-observed-from-lo-15% .- After-this-fall-,-the-
-significance-of intensity remained unchanged. Metal plates,&physiolog:
cal solutions did not evidence the presence of such a fall (drop).
The change in intensity of radiation took place in all the leaves
in the stalk and roots of the plant, In relation to the initial level.
As a result of this, the forced"pumping out" of electrons and other-
-particles-brings-about-the-gradual-decrease in number of these-particle
in the whole organism, and this fact, namely, gives the basis to
suppose the presence of an intrinsic electron-ion system in the
organism.
Another experiment: it was enough to separate the leaf from the
plant. At the moment of death, as we observed sharp discharges-of-
radiation with its rapid extinction. - How is one-to treat this fact?
Above, all, the cut-off leaf loses the stability which it possessed
in being connected with the whole plant. But, there is no possibility
of compensating the lost charges during their emission. From this,
the following regularity or conformity of such radiation or reaction
Can be seen.
"In the other experiment,-the plant-was-placed-in-a-constant-magnet
-field, the character of-the- radiation changed. The intensity was
lower_than when_it_was_acted_upon by the magnet, but the length of time
of extinction increased by 2 or 3 times. The magnetic lines of force,
hindered the easy extraction of electrons and other particles from
the plant. All this also reminded one of the behavior of plasma in
magnetic fields.
During the increase of blood temperature from 40 to 42.5 degrees
C, an abrupt increase of radiation discharge was observed, followed
by its drop. Something similar is also observed in the leaf. Would
this be the critical temperature of bioplasma stability? It is possible
that this is so. The disturbance of bioplasma sharply Increases the
possibility of the emission of the particles, increasing-the brightness
of-the-plasma radiation discharges. It is not without interest to
observe that during the heating up of the shoots of plants at definite
temperature intervals a bioluminescent discharge was observed (weak
light conditioned by: . changes in properties) .
A.G. Gurwich had, In fact, observed discharges of mitogenetic
Fadiation during
_ cheating up. Could this be a coincidence ?-
Unconditionally no! We suggest that the higher the a
bicplasma and.these2000/08/07+CIA-RDR96 00787R000500080007-of the
ey more intensive tae
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A WORD ABOUT BIOPLASMA -7
Interaction of particles comprising it. During the disturbance of
bioplasma structure a certain amount of heat should be discharged. In
connection with this, one should not fail to mention the experiments-
carried out-by-the English discoverer Hill, in 1928. Hill discovered_
that the amount of warmth given out by some organic molecules during
the absence of oxygen over a period of 24 hours is very great and.
cannot solely be explained as being the result of blycolysis and the
fragmentation of ATF, etc. (ATP ?--- Eds). According to Hill, for 1
gram of muscle (sinew) -one obtains 5-gms/cal. This exceeds by-5-times
that which biochemical processes given out during muscle death.
Some kind of unstable structure is disturbed, depreciating its .
energy in the form of heat. We assume that such a large discharge of
heat is a result of the distrubance of the bloplasma. This is the
principal acculator of energy in the organism. But, hundreds of
experiments are still neccessary to finally prove that-the-observed
"thermal-discharge"_accompanied by_the_radiational discharge is,
above all, connected with the loss (extinction) of bioplasma. Further
discoveries about the bioplasma will help to uncover many of its prop-
erties. This is why we devote to this field of discovery so much time.
Extraordinarily interesting are experiments carried out by us
which have enabled- - us to prove-a-chain-of-well-known-properties-of-
the bioplasma
It all began with the attempt to give an explanation to experiment
made by A. G. Gurwich, carried out in the 'twenties. Two roots of
different onion plants were forced together. The distance between
them being from 2-3 mm. The irradiating root (inductor) united with -
- the onion like plant were reinforced-in-a-stative (mechanical-support)
and its end was trained on the zone of intesive cell fragmentation
measured by the other onion-like plant placed .horizontally. After
this the number of cell fragmentations on the irradiated and
non-irradiated sides of the root were counted.
The experiments showed that from the tip of the root of the
inductor, radiation in the form-of-a narrow-trained-beam-was-emitted,-
which-stimulated cell division on one side of the root. Barriers made
of.quartz_glass_did not_disturb_ the_effect,_but_ordinary_glass absorbed
the radiations. This spoke of the ultraviolet nature of the radiation.
In our laboratory, we decided to check on the experiment, but
instead . of using the small root applying the radiation, we placed a
special plate covered with photoemulsion, a new-acceptor-for-photo-
-emulsion had-been developed. The-photoemulsion was protected from
the_samll root by light_filters_allowing ultraviolet to pass.
(Caption of illustration : 8: Example of noncontact registration
of mitogenetic radiation of small root of onion.)
After long searches, finally an optimal process of experimenting
was found. the photoemulsion showed the image of the object-in the rays
of-intrinsic-radiation (see illustration Nr 8 ).
Analysis has shown a small angular divergence of radiation
that is, it is possible to suggest that every point source on the object
in fact generated laser coherent radiation. This once more proved, tha
the nature of radiation is plasma in character. And the radiation
Itself is engaged with non-equilibrium distributions of electrons in
bicstructures. At the same time, it is necessary to mention that-ther
is a larger amount of excited electrons in biostructures than those
which do not possess excitability. :
The photograph shows a living small root which is conditioned by
processes taking place in the bioplasma. The dying of the small root"
brings about light radiation which illuminates the photographic-film
--
win -a large aura around
The small dead root does not.
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A WORD ABOUT BIOPLASMA -- 8
at all give exposure of the photoemulsion. It is fully possible that
the coherent-radiation-has in itself-the-hologram condition of the
object. (see illustration 9).
(Caption of illus. 9: An example of noncontact registration of a rat
liver in the wide band of the spectrum)
What efforts need to be expended in order to decipher this.
information? That this isn't fantasy, photographs of_V.P ... . Kazncheyev.
and collaborators-also-show. They have discovered a psychopathic-effe
which is in accord with the text of Diplom thesis Nr. 122 which to
begin with formulates the following way: (page 6.7) "It has experimenta.
been established that the earlier unknown phenomenon of distant
intercellular electromagnetic interactions between two cell cultures
during the action on one of them by factors of biological, chemical,
er-physical -nature with the characteristic-reactions-of-the-other
(intact culture) in the form of 'mirror' cytopathic effects, that
define the cell system as being a detector of modulating properties of
electromagnetic radiation."
(Note: the comments of the author's above may be too brief to those
readers who are interested in repeating the non-contact method of
-registration of-biofields. Fortunately, -this procedure is described-
in more detail in some other papers by Inyushin, , and these are being
translated separately and will available shortly'. The experiment
has also been done in Copenhagen and one of the authors will publish
the details of the arrangement shortly .. ) --- Eds
On the other hand, already separate work is. appearing in which the
-possibility of the building of biohelographic-models of-brain-memory-
are being considered. (Footnote 1: S. N. Braines and A. I. Suslov:
"The neurophysiological analysis of the bioholographic model of brain
memory." Thesis of the all-soviet conference on. ; bionics. N. , 1973.)
Thus, it is possible to suggest that the presentation of the
biohologram is not a myth, but a scientifically demonstrated fact.
Naturally, the fact that many experimental-proofs-are needed-in order-
to confirm this conception in science without doubt presents itself.
With the help of radiation of bio-objects we can determine the
condition of the bioplasma and this opens new possibilities in .
medical diagnosis.
The energy state of bioplasma In the narrowest sense depends on
"the breathing" of the cosmos. The biosphere is subject to the
interaction of gravitational, and other various cosmic fields. The
streams of plasma direct themselves towards the earth.
Disturbances on the sun create "noise" in the harmonic bioplasma
and at small stability , disturb it. Facts of solar activity influenci-
life are at present the property of science, thanks to the work of
the Soviet scientist A.L. Chizhevskiy.
Depending upon the position of the planets in the solar system,
the intensity of electrical and magnetic fields on Earth change. The
bioplasma cannot "cease to breathe" in one rhythm with the cosmos.
We
Hence, naturally the observable rhythm of biological processes.
reap the fruits of solar storms in the form of infarcts and insults,
the outbreaks of epidemics and so on, but the mechanisms of such
happenings are up to the present time puzzling. . (Note: infarcts:
from the latin infarcio, necrosis of organ or tissue, the result of
arrested circulation.
-
£
insult: from the Latin insultus: disturbance
of brain blood circulation e.g. artereosclerosis -- Eds). It is
possible that biplasma will enable us to clarify much better the
-affinity of life with the cosmos.
Illustration lo shows graphically how the podesoddy0001 light
radiation honderdel 20001081976-Potete in living organisms.
Approvs
Tements were taken each hour.
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A-WORD-ABOUT-BIOPLASMA
9
It was observed that a sharp increase in electrobioluminescece wa
observed at 2 AM and 6 AM. Hence, a 24 hour dynamic property of
electrobioluminesence has been discovered. By what is it conditio
ed? (Text of Illustration lo: 24 hour dynamics of the intensity of
electrobioluminescence in the skin of the ear of a rabbit.)
Specially-organized-experiments-speak of the dynamics of luminese.
as reflecting the 24 hour progression of changes in the intensity of.
the electrical field between two gigantic plates of a condenser ---
meaning the ionosphere and the earth. The ionosphere carries ac
considerable charge, the earth being negatively charged. The
rhythmical change of intensity by night is conditioned by the lighter-
emission of charged particles from the surface of the rabbit's-ear-
during research made into luminesence in high-voltage impulses accordil
to the method used by Kirlain and assistents. We have begun to learn
that this phenomenon is more profound suggesting that the structure of
the biofield should . also rhythmically fluctuate. Research has been
made into the 24 hour changes of anisotropy of the biological objects."
The ability to turn-the-plane of polarization-of-the-ray-has turned-
out to be dissimilar during night and day. What is characteristic is
that a change in the direction of the turning of the plane of polari-
zation takes place at different hours of the 24 hour day.
And só, the ordered structures of the bioplasma isn't so "crude".
The structure is changeable, and depends upon the changes of the
rhythmically-pulsing-structures-of-the-gravitational-and-electrical
field. Experiments have revealed a completely new field ---- discovering
the science of biorhythms in connection with the conception of the bio-
plasma.
The bioplasma is saturated With vibrations possessing very
sphecial characteristics, such as: coherence, and polartaation,
Vibrations in the red-band of-the spectrum being dominant. The red-
resonance is in itself a symbol of highly-organized life. That, is in
fact_why it is possible to have such_fine (precise) regulation of
biological processes, with the aid of radiation energy generated by the
helium-neon lasers.
As far back as the 30's, E. Bauer said that polarized ray emission
- should possess larger biological-activities-than depolarized light.
- However, experimental data on the role of polarization_in_photobiologic
- -
reactions is small, although the formulation of such a problem in our
days is all the more topical, in connection with the necessity of
carrying out research into the mechanisms of the activity of monochrom-
atic polarized coherent laser radiation.
In our laboratory we carried out a cycle of work on discovering
the role of polarization and coherence-in-the-biological activity
of light. It was learned, above all, about the influence_of_polarized
and nonpolarized light on breathing and photosynthesis during equivalen
intensity of light. The breathing of the shoots of plants was determ-
Ined by means of a gas analyser
analyzing CO,. The Helium-Neon lase
with wavelength 6328 A with ray intensity falling on the camera being
at-o.1-mW/ cm- was used as the source of radiation, The weakening of
the rays was achieved with the help of neutral light filters and the
depolarization of radiation was achieved with the aid of ground glass.
Depolarized radiation brings about a sharp decrease of respiratory
Intensity. The experiments were well carried out. Thus, it is
possible to speak of the large role of linear polarization, in the
-
photocatylic activity of light on the complex chain of processes during
respiration.
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A WORD ABOUT BIOPLASMA -lo
Possibly, the effect is conditioned by the electric and optical
polarity of-the-biosubstructure, about which we shall-speak below.
During another experiment, we made-research into the influence
of polarized light on the synthesis of pigments. For this, two groups
of plants were grown. One under polaroid film, another under a
neutral grey light filter. Illumination in both cases constituted
from 1000-1,2000 LK The results have been presented in table 3.
{Caption of table 3: The content of pigments in leaves, 4-day
growth, in mg %)
:
The phenomenon of accelerated synthesis of pigments during the
activity of polarized light is observed. It follows that the photo-
synthetic productiveness of growth will also be higher. The experi-
ment shows that changing the regimen of polarization of Light we
ean-directionally increase the photo-synthetic productivity. One of-
the most convenient group_indicators of photosynthetic-effectiveness-
is the rapidity of oxygen discharge. For the accurate measurements
of this magnitude, it is possible to use the polarographic method of
determining oxygen which contains in itself a high degree of sensi-
tivity, non-inertia and allowing the observation of photosynthetic
change in various-experimental-conditions.
As is known, the polarographic curve of-oxygen-appearance-has-
two sharp plateaus parallel to the axes of the potentials. The
plateau manifests itself as a result of the fact that during the
potential corresponding to Its beginning, the rapidity of appearance
reaches significance at which all the molecules of the substance, in
this case, oxygen, rapidly form (reform, restore) .. The establishment-
of oxygen is a very complex process, going through-a-whole-series
of more simple elementary stages:
The flow of the first half-wave, or semi wave, is conditioned by
the restoration of oxygen to the point of peroxidity with the binding
(joining) of two electrons. Depending upon the Intensity of the
-processes going on, the magnitude of-the restoration of-the current
changes, about which it is possible to speak about-a concentration of
restorative bodies, particularly of oxygen.
To express the results in absolute units, a periodical grading
of the cell according to the oxygen content was carried out. The rate
of-distributing the apparatus was determined according to the
- magnitude of the limiting-current, accompanied by the concentration-
of oxygen at 21 %, and comprised 9 x lo " moles/1_Apart-from that,-
before each measurement, the volt ampere curve of pure water, or buffer
was checked, where the object experimented upon was suspended. The
concentration of oxygen in the water within the radius of the cell was
:0025 x to moles/L . In the series of experiments made to learn
about-the influence of polarized-and-depolarized laser light, kinetic .
- curves of oxygen restoration were obtained, according to which it-
is possible to speak of the change in the rate of photosynthesis.
The experiments were carried out according to the following
Scheme :
1) recording of the Initial state of chloroplast suspension
2) activating the laser and simultaneous recording of the curve of
oxygen. restoration
3)
Laser illumination in the period of a definite time period (10-30 :
4)
the depolarization of radiation
As is the rule, in initial conditions the concentration of oxygen
constituted from 0. 7% -0.75 x 10
moles/L .
With the activation of
the laser, a decrease of initial level to 0.675 x 10
moles /L has
been observed, which
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Thus, in a period of lo-minutes , the concentration of oxygen increase
and constitutes 0. 77 x lo_moles/L -- And-after-the elapse of
30 minutes , 1.65 x lo -
moles /L. If the suspension-is-illuminated
with depolarized laser light, then the curves sharply decrease, some-
times below the Initial level of 0. 155 xlo moles / L .
Changing the scheme of the experiment ---- Illumination by depolarized
and then polarized-laser-light-brings about a similar reaction.
We give here the mean data of the experiments carried out
in moles/ liter:
Inital level of oxygen concentration --- o. 75 xlo-3
depolarized laser light
0. 70 x lo 3
illumination of suspended object
by depolarized laser light -- over
a period of 30 minutes
0.29 x-1 -3
polarized laser light
Q.775 x lo"
-3
In another series of experiments-the task was to explore the
energy output of photosynthesis_where use was made-of-He-Ne-lasers-
as coherent polarized beams. For this purpose,_a_photocalorimetric.
method of a gradient-type was applied, whose advantage was its great
-sensitivity (0.002 degrees C) and which is extremely important in
biological research. The temperature of the object being able to
insignificantly vary from-the-
temperature of the surrounding
medium. The working principle of the_calorimeter-is contained-in
-
temperature changes of the suspension of chloroplasts during laser -
radiation and in remeasurement of temperature of the same radiated
suspension, but in non-photo-synthesized conditions. . The differences
in-temperature of non-photosynthesising (large T ) and photosynthes-
ising_T _suspensions are proportional to the amount of energy
stored during photosynthesis. The energy outputis :
E
-=
T
T
p
nps
The amount of-absorbed energy of laser radiation was measured"
with the help of IMO-2-and-comprised 8-x-lo"-ergs/sec .. . "During the ".
radiation of the chloroplast_suspension by laser, -the-temperature --
curve fell. At the same time, a part of the light_ energy degenerates
"Into heat, raising the temperature of the suspension and the cell.
-The -more intensive the photosynthesis, the less absorbed light
degenerates into heat, --
Th
at is , the more intensive the photosynthesis, the ."colder"
"the-illuminated growth will be during the remaining equilibrium
conditions. The energy output-is-calculated according to the
following formula:
-RART
T
temp
1
where E = 1, and R.
= 8 X 10
erg/sec
R.
2 is the integral thermal effect at-the-moment of determination during
the action of the light, K is the heat capacity of the call-at-the
calculation of the quantum emission (output) of photosynthesis
-
expressed as r/a, Where r = the amount of emitted oxygen in moles
:- the energy of the optical radiation in Einsteins of absorbed.
photons. We find that this amount correlates with. the magnitude of ..
energy output: the quantum output of photosynthesis during laser light
artcation approached
2
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A WORD ABOUT BIOPLASMA --- 1-2
In this way, while studying photosynthetic and nonphotosynthetic
action of light, it is neccessary not only to calculate the intensity
and spectral composition, but apparently the character of polarizatio:
and coherence of light radiation.
In the laboratories of biophysics of the faculty of genetic of
growth of the academy of sciences of the Moldavian-SSR, and in the
Kishinev agricultural institute S.N. Maskobrod carried out original -.
research into the change of
-
biopotentials of the leaves of
corn when they were radiated with light with various wave character-
istics. (footnote 1 : S. N. Maslobrod. "The electrophysiological
polarity of plants" Kishinev,-Shtiintsa-publications 1973) It-
transpired that polarized coherent_light creates the strongest
photoelectrical responses in comparison with
noncoherent and-
nonpolarized light of equivalent intensity.
All this once more confirms our point of view about the role
of-polarization and coherence of radiation in photobiological react-
ions .. Namely,-that the variation-of-space-time characteristics of
radiation allow the establishment of a specific stimulation-of
biological processes thanks to the_resonance mechanisms of interaction
with the wave structures of bioplasma. The creation of lasers. to.
Work on predetermined frequencies has opened new possibilities for
the induction of bioresonant effects. Not far off is the day when
stimulated_radiation-of-the biological part of nature will be-
obtained. It is then that the science of biofields will -liberate
itself from the accumulation of hypotheses . and will become the
arena of unique experimental research, the results of which will also
"lead medicine towards basically new horizons .:
It is now, that we are just beginning to penetrate this earlier
inaccessable sphere of-knowledge.
.
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Рис. 9. Пример бесконтактной регистрации излучения
печени
крысы в широком диапазоне спектра.
Illustration 9: An example of non-contact
registration of a rat liver in to wide-
band spectrum.
-
Рис. 8. Пример бесконтактной регистрации
митогенетического излучения корешка лука.
Illustration 8: example of non-contact
registration of mitogenetic registration
of small root of onion.
Illustration 7: Corona incandesonce
of human finger.
..
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BIOSTIMULATION WITH THE AID OF LASER RADIATION THROUGH
-ACUPUNCTURAE-POINTS -- Chapter VII
For a comparatively long time the position has been held in
medicine that the appearance of-pathological processes are connected
predominantly with the disturbances of the nervous system. A. D.
Seransky has studied the causes of and mechanisms of the
development
41
of nerve dystrophies. . It has been demonstrated that the reflex.
mechanisms are the cause of a series of illnesses: . Well known are the
classical experiments carried out by M.K. Petrova who was able to
show-how chronic nervous tensions create a whole series of pathological
states in dogs.
Academician K.M. : Bikov revealed the close interdependence between
the core of the large hemispheres and the function of the innter
organs. : The Bikov school showed that exceptionally interoreceptive
irritations can bring . about. the appearance of heightened excitation
-in ganglion isolated area. A-characteristic viscous-circ.le-or
pathological reflex appears. and_so_the initial functional disturbances
can lead to a phase of deep organic damage.
There is no doubt about the fact that the nervous system is one
of the all-embracing systems-whose role in the pathogenesis of many
illnesses one may regard as the dominanting factor. It is enough to
-show-that-the nervous system supports the homeostasis of the trophic-
-
of the organs. (A. D. Spiransky 1935). It is known that the hemo-
dynamics of the nervous -system is closely related to the functional
status of the system: The regenerative processes come also under its
control. The Idea of nervism in medicine, is at present being
successfully developed. (G.I. Kositsky, V.M. Smirnow 1970) . It has
been-demonstrated that the leading role of the nervous-system is-
apparent_in_a_whole-series_of_pathological conditions ._ Apart_from this
we believe that the most optimum place, for prospective application
to the organism of low intensity characteristic radiation,. for example,
laser radiation, is the nervous system with its numerous receptor
elements, conductors and peripheral centers, and entire sections of
the central-nervous system.
We have already spoken of the lipid membranes having semiconductor
properties. The activation threshold of such membranes lies in the
red light spectrum.
The existence of the most probable path from the conduction band
to-the : valence band is shown by. the bioluminescent phospholipid
membranes in the red part-of-the-spectrum. The phospholipid-membranes-
are the basic builders_of_the material_of_the nerve tissues and neurons
It is clear that red light of even low intensity should exert an
activating influence on nerve elements. - The activation, like the
primary process, will .manifest Itself in the increase in concentration
of the free charge carriers, the improvement of conductance, and the
change-in a whole-series of physico-chemical properties of the membrane
peking into consideration the comparatively deep penetrative
properties of laser radiation into the core of the tissue, it is
possible to subject to radiation by laser light of low intensity areas
of the medulla oblongata, the spinal cord .and also areas of the cortex
of the brain ... In this connection, laser light appears to be a quite
convenient means of-changing the energy balance of the nervous system .-
The_Special experimental_work has_shown_the_phase_character
of the reaction of the medullar. section of the brain during the activit
of low intensity laser light, > = 6328A. ( The part of the brain
responsible for autonomic functions such as respiration --- Eds). In
the first. variant, the frogs were radiated only with dissipated laser
light, wavelength 6328A, intensity orolmW/cm , and in the second,
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intensity Imw per
cm sq. The data obtained demonstrate the existence
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BIOSTIMULATION WITH THE AID OF LASER RAD THROUGH ACUPUNCTURAL POINTS-2
:
replicating
. reaction (of the brainstem) to radiation. The
-amplitude of the reaction increases somewhat-when the length-of-rad-
iation is increased from 1-2 minutes. When the part of the brain
mentioned is subjected to local radiation of greater intensity, the
reaction takes place with some other variations of phase. To begin
with, an increase in sorption is observed.
Thereafter, its decrease.
However over a period of one hour, the magnitude of sorbtion still does
not reach the normal level.
5
Consequently, it is wholly evident that the presence of physico-
chemical changes in the medulla oblangata of the frog both during
activity and through the skin surface as well as
directly on the
brain are evident.
At the same time,. It is an indi putable fact that the appearance
of-nidus (Focal point ---- Eds) of-persistent-stimulation after-radiation,
the degree of which varies according to the sum total dosage of
light impulses. As is known, N. Ye. Vvendenskiy, as far back. as 1901,
on the basis of experiments made on the nerve altered. by various
physical and chemical agents arrived at the conviction that apart from
stimulatory waves, a gradual, long-term stimulation exists. He called
-a similar-state parabioses. Such protracted-stimulation-ocurring in-
the medulla oblangata is capable of creating beneficial conditions for
the removal of the "pathological dominant", from the vascular control
center, and also decreases the intensity of pathological impulses
from the periphery, which is of no mean significance in the treatment
of hypertonic ailments.
Of great interest-are the data relating to experiments carried
out on the isciatic nerve of a frog. The nerve_was_subjected both to
polarized and unpolarized light of equivalent exposure and intensity.
During polarized light radiation, the viscosity was. 1.5 times higher
than during non-polarized radiation (Table 4) :
"TABLE 4: The dynamics of sorbtion (relative units of extinction) in
the isciatic nerve of a frog subjected to He-Ne laser light.
5
RADIATION CONDITIONS
TIME AFTER RADIATION
M tm xlo
P
control
5 minutes
68.3 + 10.3
0.001
lo minutes
165.0 ± 49.3
0.1
1 hour
195.0 ± 36.5
0.5
polarized monochromatic
red light
5 minutes
203.0 ±15.1
0.001
10 minutes
154.0 +39.4
0.1
1 hour
221.0.+13.5
0.5
unpolarized monochromatic
red light
5 minutes
146.
$0.01
lo minutes
109.0 ± 10.2
1 hour
540.0 + 177.7
: :
Consequently, polarized light is physiologically more active, and
brings about stationary stimulation. Similar sorbtion Increases are
also produced by unpolarized red light, but longer periods. are required.
It is possible to suggest that polarized light, is more biotic and
accompanies the normalization of bioenergetic processes in the membrane.
Special theoretical and experimental research has convinced us
that a significant quantity of biotic frequencess are. situated in the
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effects manifests Itself especially distinctly
in this
part of the
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BIOSTIMULATION WITH THE AID OF LASER RADIATION THROUGH ACUPUNCTURAL
(Note: antagonism: interference with, or inhibitions of an organism
by the creation of unfavorable conditions --- Eds)
This requires, naturally, higher monochromaticity applied to the
acting radiative agent during therapy. The gas laser generates
uninterrupted (continuous) radiation. With the application of-glass
-
fiber optics, the laser radiation energy can be transmitted to the
most dispersed points on the body with tolerably high accuracy. The
utilization of He-Ne lasers is conditioned by the fact that they
generate red short-wave light, 6328 A, as many observations have shown
wavelengths varying from 6200A to 6700A produce the greatest phystolog-
ical effect during photosynthesis and the activization of oxidizing-
-phosphorization, and other fundamental bioenergetic processes.
:
The polarization and cohereree of radiation produces a supplement-
ary effect.
·
The apparatus used can be. variously constructed, beginning with
apparatus for local application and ending with: light ray baths-
used for-total-radiation treatment-of-the organs or-patients ....
As we have already pointed out, a basis exists for the assumption
of future perspectives of utilizing the spectral lines in the red part
of the spectrumto bring about bioenergetic effects on the human
organism. The most physiological, and consequently, bioenergetical
resonance lines, are those situated in the short wave band of the red-
light spectrum.
The action of monochromatic light photons brings about the mig=
ration of quanta in conductive zones which effects a change in the
energy balance of the organism .. Apart from this, photons generate
exitons and electrons, for whose transfer small amounts of energy are
neccessary to carry them to conductive zones, that energy being".
equal to that of the photons in the red part of the spectrum.
Consequently, the energy balance changes in the organism which is
fundamental for the normalization of the physiological and morphologica
state which exists depending upon the persistence of energetic
parameters of the organism.
The migration of charges along the conductive channels which are
predominantly in the form of nerves creates the foundation-for-the
realization of the main requirement of bioenergy therapy: the energy
"suboscillations" creates corollaries for the normalization of the
energy balance of the organism, and consequently, the regulating
systems. What is bioenergy therapy? Bioenergy therapy is therapy by
an energy agent whose qualatative and quantitative parameters are
situated-within-power and-spacetime limits-of-energy-process-
relationships_taking place in the cell.
Laser therapy is a particular case of widely applied reserves
of bioenergy therapeutic means. . The utilization of short-wave red
light spectral lines has many perspectives in connection with the fact
that their parameters Ile extremely close to those of the most
fundamental-bioenergetic-processes-taking place in the nervous
system (red-resonance) . There can be no doubt about the fact that in-
the not-to-distant future, perspectives will appear for the induction
of bioresonances in the green, yellow and violet bands of the
spectrum.
-
The main principle of modern medicine is based on the application
of agents -which by their nature are foreign introductions into the
organism and to a lesser or greater degree, have a toxic influence
upon (antibiotic properties. ) At the same time, other methods of
- :
therapy exist, for example, the utilization of properties that are ..
intrinsic to the organism (hormones, microelements ...
vitamins, etc.)
thera
is a-biotic.
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Low intensity therapy in the
visible and infra-red
bands is biotic ray therapy. Such light action does not produce
any new processes in the organism, but either weakens or increases
the existing ones. Light action stimulates the cell elements-and
nerve conductors. But it is neccessary to observe that this
stimulation in is not unlimited, and can enter an arrestive phase.
Thus, the phase character of stain sorbtion by the medulla oblangata
or iscial nerves demonstrates the development of the so-called
parabiotic conditions during the summation of light impulses attaining
a definite magnitude.
According to the opinion of N.S. Vedensky, parabiosis is a
-
thorough stimulation, that is, a local, persistant, non-distributed
stimulation. In the first (level) phase, the nerve loses its
..
ability, in various ways, to react to strong.and weak stimulatory
agents, including light agents. Further, the nerve responds to
the action of weak, and not to strong, factors. As-the precess-is
lengthened, an arresting-stage-may appear, in the absence of
an external visible reaction_to_the_stimulant used: Consequently,
when selecting exposure and intensity it is ..
neccessary to work
out the initial functional condition of the patients. With the
presence of. initial phases of parabiosis the reaction will be
proportional to the length and intensity of stimulation. It is-
-also indispensible-to take into account a-parameter such as lability.
The more labile a nervous system is, the lesser the dosage neccessary.
Here it is probably neccessary to follow the recommendation of I.P.
Pavlov: "There is no doubt that. the dosage has a much greater
significance downward than .Upward. . The whole art of varying dosage-
is downward. " (Pavlov Clinical Proceedings, Vol I, 1954, pp. 79)
In order to select the optimal-exposure it is indispensible to carry
ou7
careful clinical and functional analysis, Particular attention
should be paid to the lability of the vascular system and the
electrophysiological indicators. . During large amplitude changes in
the filling of blood in vessels, the amplitude and frequency of
biopotentials should proceed to minimal exposure and intensity
It turns out, the results of electrotherapy can find wide
application since the root of most ailments lies in the disturbance
of the neurovascular trophic.
Naturally, the best results should be expected from neurogenic
illnesses and inflammatory processes as well as disturbances of
the neurovascular trophic. Positive results are to be-expected-from-
the various disturbances (arthroses) of serious neurological illnesses.
Their use is not recommended in states_of_acute vascular disturbances,
pre-insultave or pre-inforctive states, acute inflammatory processes,
with the appearance of sepsis, and various other diseases, when
stimulated, may.bring. about.undesirable consequences.
Quantum generator radiation therapy should be carried out in
conditions which would be inducive to the maximum amount of
bioenergetic resonance .:
:
To achieve this, a specially equipped isolation chamber is
required. for. the isolation.of .. electromagnetic.and sound waves, and
maintenance of constant electrical parameters of the medium. Usually,
in practical therapeutic work, and during experimental investigations,.
the electrical state of the air in the chamber is not taken into
account. Such a situation is undesirable in the case of laser ray
therapy, Accepted as a norm, are 150-280 light aero-ions in one
cubic cm of air. For this purpose it is possible to utilize an
electrofluvial point-tip ionizer producing up.to 5000 aero-ions
per cubic cm, regulating the number of tips on the filiment it is
possible to achieve a regular amount of ionization in the region
3
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In the chamber where radiation takes place, it is necessary to
maintain a weak light-green or twilight lighting. Light isolation
prior to the procedure is carried out for lo minutes. Consequently, the
camera . serves as a means to creating conditions conducive to the
-energetic calm of-the patient's organism .. In connection with this
only the inner endogenal pathological phenomena will bring about
various disturbances in the bioenergetic system of the organism, i.e.
non-stable characteristics .. The problem of procedure timing also
plays a certain definite role ..
Apart from the bioenergetic processes, the most effective
procedures-are nocturnal from midnight te 1 AM .- In this period,
it is possible to obtain the most pronounced resonance effects within
a few hours after a single dose of radiation ... However morning procedure:
from. 9-lo AM.are possible .. The anisotropic bioplasma is subjected
to the greatest changes during the night from midnight to 6 AM.
This is a period of least bioplasma concentration, -and that is why
any weak-action will be more effective .-
Such diurnal rhytym of activity should be observed, especially.
during radiation of sensory nerve centers. It is known that night
constitutes the "kingdom" of the vagus, for during the day, the tone
of the sympathetic nervous system increases.
At present about 40 physiological functions are known, subjected
to precise-cyclical-changes with a period-approaching 24 hours.
(N. A. Agajanyan, 1967)
:
..
One of the important problems is to localize the activity.
In physiotherapy, the discovery of the reflexogenic zones which
insure the specificity of reaction of the various organs has found
recognition (A.R. Kirichinskiy, 1959) . Quite convincing physiological
interpretation-has been given-to-the link between the inner organs-and-
skin surface. At present, no one is in any doubt that the processes
taking place in the internal organs are reflected in the perpheries
of the skin surface. The connections between the organs and parts of
the body are of various type: taking place through the vascular,
lymphatic, or nerve systems. It follows that it is possible to obtain"
a whole series of response reactions on the part of the internal
organs when the_reflexogenic zones subjected to the physiofactors
gives The effect of a decrease in arterial pressure. Functional
changes on the part of the inner organs of the minor pelvis are
observable during action on the area of
, however it is
-
neccessary to take Into account that the principle of strict-
segmentary innervation like that of the surface of the body as well
as the internal organs are_ not wholly supportable, that is ,
the stimulation does not only spread. in the area of this or that segment,
but. has its. points. of departure beyond its confines. This is
conditioned.above all, by the fact that some peripheral nerves or
trunks comprise the fibers of several roots. All this disturbs the
segmentation of body- skin surfaces connected to the spinal braincord.
For_ : hypertonic therapy purposes using the action of laser
light, we chose a series of reflexogenic_zones known_physiotherapeuticall;
The most effective one is the gate. zone (portal).
The other method of action on a pathological nidus is that of
photopuncture. That is, the action of laser rays on the acupunctural
points, localized on the surface of the skin.
At present, there is no concurrence as to the biological nature
of acupuncture points. Chinese doctors as a result of thousands of
years of painstaking observations have discovered six hundred and
ninety three points on the human body, which if stimulated, produce
:
fully consistent physiological reactions and therapeutical effects.
Accoring to Japanese medical literature, there are in all 120
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THROUGH ACUPUNCTURAL_POINTS-6
Some authors suggest that in the aspect of ontogenetics, that
these points are the relics of atavistic (ancient) factors in
stimulating the sytem ontogenetically speaking, which are intricately
connected with the-nerve centers.
.
V.G. Vogralik (1961) suggests that the Chinese points represent the
greatest activity on the system of "skin surface -- internal organs"
interactivity when projected on skin sectors. They contain nerve
receptors carrying the stimulation in a centrifugal direction.
A.K. Podshtbyakin (1960) concluded that the skin nerve points
ef frogs;, rabbits ,-dogs and human beings correspond to-points-of-
bifurcation in the nerve trunks, especially in the area where they
eneer the-skin .. According to him, the measurement of characteristic
.physical active points is of great diagnostic value.
Data have been obtained about the majority of acupuncture points
possessing higher electroconductivity. (V.G. "-Adamenko and V.D.
-Raykov 1969) -Special apparatus-has-been built to determine -the-
electrical resistence.
There has been talk about regarding the nature of acupunctural
points as being semiconductive.in nature. . (D.L. Parmenyenkov, 1970)
Thus, a. typical volt-ampere characteristic was obtained, of a distinctl
non-linear character. As a result. of many determinations of electrical
resistance magnitudes in acupunctural-points-in-humans-and-animals,-
a distinctly significant statistical dependence of this magnitude-on-
the polar character of the voltage applied_was discovered ( it was
of a semiconductive nature) .
In recent years, there. has been a considerable growth of interest
"in research made into the basics of acupuncture -- the fundamental
-principles. To demonstrate this,the ministry-of-health-protection-of
the USSR has decreed that it is necessary to develop research in this
direction, and in 1972 an all-Soviet conference on acupuncture problems
was held in Leningrad ..
During the past 5 years, a series of experiments were carried out
to discover the biophysical nature of acupunctural points and their
interrelationships, at the republic-ministry of-health-clinical-
hospital in the Kazakh SSR
Laboratory assistants V.A. Hruschov and M.A. Vorobyov came
V forward with some interesting concepts. on the mechanism of the
therapeutical. effects through acupuncture points." They suggest that
normally functioning biological systems possess the intrinsic
condition-ef-entropy balance expressed in the formula:
as, /aT + ds /aT = 0
where ds, /dr is the rate of entropy change due to the forces disturbing
the system from equibilibrium, and.dS /dT is the rate of entropy
change due to activity of forces regenerating the system. In the case
of pathological changes in the system, the entropy balance is disturbed
due to the increase of D ds, /at or the decrease of ds, /dr. This
leads then to the inequality ?.
7
0
(i.e. the irreversable process == Eds)
The disturbance of the entropy balance is accompanied by changes
in subject resistance. With the increase_of_entropy, the resistance
begins. at first to drop to a certain minimum magnitude, and then
increases and reaches a significant point many times greater than the
initial one. (A.K. Prits and M.N. Cherkas, 1970) .
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STIMULATION OF REGENERATION BY MRL. . - 7
disturbance of-a-root-type (binding ?- Eds). The majority of patients
had constipation and a disturbance of urination. In 3 cases, bed-
sores were observed, Clinical laboratory research showed that in
3 cases, leucocytes numbering 9-12 thousand were observed. The ROE
...
increased from 25 to 60 mm. . In a number of cases, sub-fibril
temperatures were observed.
Prior to the patient's entering the republic clinical hospital o
the ministry of health of the Kazakh SSR, they-underwent a long-term
concentrated treatment, consisting of disinfecting and dissipation
:
methods. (medicines) and sanatorium treatment ...
However, in 6 of the patients, all the methods of treament
enumerated above did not.give any positive results. An, in Inly 3 We:
any permanent improvements observed. - Thus, the existing methods of-
treatment of-myelitis-turned out-to-be-noneffective .- In connection .
with this,_taking into account_experimental_data_all_patients_were
subjected to courses of treatment of He-Ne laser light on "actove
skin points" and reflexo-genic zones. Treatment a was carried out
in a special chamber, which was protected by an electromagnetic scree
The results of treatment with the aid-of laser radiation carried
out in-a period-ranging from 20-25 days, surpassed all-expectations ---
First, after_the course_of_therapy_was_completed_the_pain_syndrome_
completely disappeared. During and after treatment, a decrease in
vegatative-trophic sistrubances was observed, a-healing of bedsores,
an Increase in the elasticity of the skin, a decrease in the
brittleness of nails, etc. All patients experienced a considerable
improvement in their somatic state. In 2 a cases,-an improvement in
the kinetic functions was observed. . ( I patient began to move about
on his crutches, and another abandoned his crutches, and began walking
with the aid of a stick.)
Such are the experimental data and clinical observations
demonstrating that under the influence of low-intensity-monochromatic
coherent polarized radiation, an activization of the energetic pro-
cesses-takes place_in the nervous_system. The frequencies_in_the
short-wave band of the red spectrum turn out to have an energizing
influence on nerve receptors .. A Significant role is played here by
the second harmonic lying in the sector of mitogenetic radiation and
created-as a result of the absorption of 2-photons-of-red light-from-
a-
:- He-Ne OQG.
-
Experimental and clinical data, presented above, contradict
the views still held on the non-specific thermal character of the
action of electromagnetic radiation.on the organism of animals and
man. What are the mechanisms of non-thermal effects of laser radiat=
ion? These questions can be answered. after acquaintance has been
made with the concept of bioplasma, through which the action of
electromagnetic radiation is effected.
A completely new field of directed regulation of the bioenergeti
state of the organism is being discovered. The resonance bioeffects
induced by radiation force us to create new concepts about the living
organism, as being a whole, in which the energeti 'c. processes lie-at
the foundation of all manifestations of life. The concept of bio-
plasma has been formed, which better helps us_to_understand_the_causes
lying at the foundation of life, and opens new perspectives for the
directed influence on normal and pathological processes.
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STIMULATION. BY :RADIATION --- THROUGH-ACUPUNCTURAL_POINTS -7
3
.1
The many bibliographical .data on the measurement of resistance
of acupunctural points show that, in pathological cases the points
Utilized. in therapy have a resistance that is many times smaller than
that of the skin. And in chronic cases, their-resistance attains
-magnitudes exceeding that of the skin. After treatment of illnesses,
the resistance of the points is restored. ( . E.S. Vel'hover 1967,
M.K. Geykin 1970, B.X. Shuyskaya 197o) .
The dynamics of point electroconductive changes from normal to
pathological. states and again. from pathological to normal, give groun
to assume that these changes are connected with the disturbance of-
the entropic balance not only in the pathologically changed organs~
but also in the points themselves. The parallel changes in balance
in points and organs are explicible by utilizing the grasmic memory
model ( V.A. Hrushchov, 1972, 1973) .
:
In contrast to other memory modesi found in the literature,
the grasmic model ensures-the time-measured disclosure of the
incoming information beginning with symptoms ofgreat est intensity
which is very important in the present case .. . (N.M. Amosov, 1964,
and A.A. Bratko 1969) .. . The ememorization of information takes place
in the memory cells, conventionally termed acceptors. The nearest
analogy to acceptors are neurons, but they different from them because
of their-basic-simplicity .-
An acceptor is a junction in which connections from and to it
are led from other; similar acceptors. It is characterized by its
stimulatory potential, dependent upon the number and intensity of the
signals of inhibition and stimulation going through the connections.
Allowance Is made. for signals to go in an opposite direction but with
"great-suppression (reduction) . Stimulatory and inhibadory signals-
passing through the acceptor correspondingly stimulate or inhibit it,
and becoming weaker continue. further along the connection.
: =
..
There are two basic types of acceptors: 1) primary acceptors
which do not manifest themselves in the information memory processes.
Their sole connection is either with a dator accepting Information
or dators-from which-information-is extracted. 2)-Secondary acceptor
manifesting themselves in the process of memorizing information
(storage).
The intermediary link in the creation of a secondary acceptor is
a donor. A donor is a periodically .occurring Inhibitory signal in"
a random point situated in the memory block area In places where
there is an absence of connections-and-accepters. This inhibatory-
signal which removes the potential (depolarizes) from the most
stimulated acceptors, forms a link with it .. After the formation of
the link, the inhibitory potential. jumps to the potential of stimulati
and as a result, the potential is restored to the most strongly
stimulated. acceptor.
The primary and secondary potential acceptor differences-form
a picture reflecting the character of the signals received by the
model. The inhibitory signals coming from the donor erases this
picture as though .concentrating it in a new acceptor on account of
the links formed. : The formation of the next acceptor is characterized
by the fact that apart from memorizing the new picture, it always
creates a strong link between the previously formed-acceptor on accour
of its strong stimulation. Depending upon the specificity of balance
disturbed, the chain stimulated is the one that is most strongly
linked with the most stimulated primary acceptors. The circuit of
acceptors formed reflect the consecutiveness of change of the stimul-
ated pictures.
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STIMULATION BY RADIATION---THROUGH ACUPUNCTURAL POINTS-8

It is not difficult to see that, if in the process of memory creation, no signals arrive, then the consequent memorizing of pictures will bring about the doubling of previously registered chains. Depending upon the strength of links, this process can spring from one sector of consectutiveness to the other, omitting or gradually settling on the very same picture. Thanks to the occurrance of the inhibiting stimulating signal ., the picture formed where the acceptors appear does not only reflect the signals coming in, but also [Handwritten interlinear insertion: "Those"] that arrived earlier. That is why the process of registering and distributing information in the grasmic memory model is connected to a unified whole, which apparently is very convergent with the processes that take place in biological systems.

On the bais basis of this model, let us now consider the connection between the points and the organs. We have a memory block, in which there is a large amount of acceptor circuits, appearing in the development process of the biological sample, they reflect the infor-mation of the entropy balance of the organism. From this number, it is possible to extract a group of chains (circuits) which are connected by means of the primary acceptors with the organs in question, and their corresponding acupunctural points.

When the entropy balance in the organ or point is disturbed,a stimulatory signal appears on the primary acceptor and [blank gap in typed line] further on , on the group of circuits. Depending upcn the specificity of balance disturbed, the [blank gap in typed line] chain stimulated is the one that is most strongly linked with the most stimulated primary acceptors. As a result of distributing the information down along this chain, the inhibitory signal reaches the active dators, whose work restores the entropy balance, then a pathological process appears in the corresponding organs and points, which is reflected in the resistance of these points.

The stimulation of points during therapy increases the disturbance of the entropy balance within them ,and consequently , increases the stimulation of acceptor groups and points connected to them. The increase in stimulation leads to the increase of the inhibitory signal normalizing the conditions of balance in the very point and organ. This is what achieves the therapeutic effect. The restoration of balance takes place more rapidly in points as well as in less-organized systems, then those which organs make up. The process of normalization takes place very gradually, which points to the complex-ity of the physiological processes restoring the balance.

The present model allows the restoration of the entropy balance during the action of the acupunctural points, not only in the organs, but in the the acupunctural points, since the connection between the point and the organ as well as the point-to-point connection are the same in the modelling process.

In the "Aksay" ministry of health child clinic of the Kazakh SSR, B.Z. Shuyskaya investigated the dynamics of the skin galvanic (?) reactions of points. The apparatus used in the search for acupunctural points and the quanitative evaluation of their electroconductance was V.G. Adamenko's (1967) "biometer" composed of 2 electrodes, one of [stray mark resembling an apostrophe after "of"] zinc and the other of brass, connected to a microammeter--type M-13 [line ends at right page edge; a trailing character may be cut off] M-265 M, M-266M, M-95. During the search for points, the passive Zn electrode was attached to the hand of the a patient, and the other, active brass of copper electrode clasped in the [blank gap in typed line] hand of the experi-menter was rolled along the skin of the patient. The moment the acupunctural point was discovered, the microammeter needle deflected, revealing the circuit formed between the Zn electrode, the conductive channel, and the brass electrode.

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STIMULATION BY RADIATION. .. THROUGH ACUPUNCTURAL. POINTS=9
.
-
On account of the electronic level differences in metals (i.e.
work function of the metal -- Eds) a continuous EDS of points also
appeared (electro-dermal-signal? -- Eds) dependent upon the channel
resistance adjusted on the scale of the apparatus (i.e. calibration-
Eds)
For purposes_of_quantitative evaluation of resistance it is
indispensible to work out the most optimal measuring conditions,
since the amount of resistance. depends upon the degree of skin moistu
and the pressure of the active electrode on the skin, etc.
The following methods were used in measuring. The skin of the
patient was rubbed with spirit The zinc electroce was wrapped in
muslin, soaked in_a_ physiological_solution for the purpose of increas
ing sensitivity. If a low-sensitivity microammeter of the M-130
or M-265 M type is used, then the search'electrode is slightly
moistened. When both electrodes are moistened, sensitivity increases
by a factor of 3-5. The most sensitive apparatus is the M-266M
with an amplifier. Thanks to the considerable-amplification, it is
possible to work with dry skin without the need of moistening it.
The constant pressure of the electrode on the skin is very
important in the measurement of electroconducaance .... We made use
of a constant-pressure electrode Which employed a spring mechanism.
For the registration of-electroconductance in a dynamic state,-
we utilized a microammeter type M-95 with shunt, Electrodes in the
form of Ag and Au discs of an area of 0.25 cm" were used, which wer
placed on the acupunctural points .. After the 'sk in-of the patient
was cleaned.with spirit, the .neccessary points were found, onto which
a conductive paste was applied, and then with the help of
leucoplaster, the electrodes were fastened en.
: This method permits the registration of simultaneous resistance.
changes in the various channels during the whole procedure of laser
radiation, which is very important for the evaluation of the reaction
of the patient to radiation ..
Indispensible. are further efforts to find ways or working out
the most optimal means of measuring the electroconductance and-the
creation of-sensitive-apparatus that-can-be-more conveently used.
One of the ways to objectively evaluate the_initial condition and
reaction of the organism to radiation is a.modified skin galvanic
reaction, registering changes . in electrical.properties. . A similar
method, in the evaluation of emotional reactions, in both normal and
hypnotic states, was applied by V.G. Adamenko and V. LRA ykov (1968).
During-therapy of child-cerebral-paralysis, a-monochromatic red-
light_He-Ne_laser, LG-75, 6328 A, radiation_power_25 mW/cm", was used
Radiation was applied continuously with the aid of a concentrated ray
which was dispersed by a light-training device along the acupunctural
points. (T.M. Shakirov, 1972) . The skin galvanic reactions were
registered from acupunctural points on face, hands, and legs as
reccommended - The-registration of electrical signals from the points
was EPP-09, enableing the gradual dynamical processes or normalization
of locomotor apparatus functions of the sick children to be
monitored .: The electrodes were put onto a paste for better skin
contact.
.
.
15 children, ages 3-12 were examined. 14 suffered from congenit
encephalopathy. Of thesee, 11 from an extrapyramidic form, 2 from
a pyramidic form, 1 from a mixed. ' form and. ]from tortial distony.
Depending upon the disease, the following. points were taken for reg-
istration: .. sken'-men' , Jau-Huey, shao-tse, tzyan-yuy, vey-guan',
.
ney-tin, bi-quan! .. . The skin galvanic reaction. registration was done
over a period of 15 minutes prior to and after radiation on the first-
day, the 3-5th day, the 9-11th days, and after 25 procedures.
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STIMULATION BY RADIATION. .. . THROUGH ACUPUNCTURAL POINTS
10
The curves obtained give a presentation of the dynamics of change
of the whole complex .of electrical properties in the organism of the
child. Electroconductivity and the laser-Induced potentials change --
comparatively slowly and the -muscle potential-action is distinguished
by rapid changes The muscular_potentials connected with the activity.
of the muscular_system_ _ permits the evaluation of the physiological
activity of muscles, which is very important for the determination in
objective terms of the patient's condition, When suffering from
2.
hyperkinesis.
In healthy children, in a tranquil state, the curves obtained
-
during registration were characterized by slow waves. During muscular
movement, and its voluntary_interruption, high_amplitude_and_frequency
vibrations appear on the background of the slow waves, which are
characteristic-of muscle potential action. In healthy children, the
Character of the curves is more symmetrical, with sloping crests and-
gradual. tapering off. . In the republic specialist-"Aksay" child-clinic,
T.M. Shakirov and-B.Z Shuyskiy registered-many-skin-galvanic-reactions
from "active" points on the skin in healthy children, and they all
have converging_elements (seem to coincide).
In patients with. acute symptoms of hyperkinesis, and changes in
the tonus, many different kinds of electrogenic disturbances have
"been observed, different electrical syndromes and phenomena take
place, differing from-the-norm-(L.S .- Petelin 1970) .- Characteristic-
of such patients is the high-frequency_component.which reflects a
condition of muscular electric activity during voluntary interruption.
L.S. Petelin (197o) observes that patients with hyperkinesis possess
an increase in skin. galvanic amplitude reaction and frequency of
muscular vibrations (contractions) generally taking place in the
form of-salvos (spasms) -- developing synchronously. According to the
degree of synchronization it is possible to conjecture the lability
of the neuro-motor units, channeled into the motor activity.
In the majority. of curves, a certain periodicity of electrical
processes can be traced, which take on the form of pulse rhytyms,
a series of pulses is observed with the fall in electrical activity.
Quantitative-differences in amplitude,duration of-pulses, and
frequency_of_ bir_vibration_is_observed ._ Unfortunately, due to_ the_low
speed of the registration, it is not possible to say anything about
the frequency of vibration and the degree of synchronization.
As far as the amplitude of muscular vibration is concerned, it
decreased from 3 to 5 times during the process of therapy. The
decrease in vibration amplitudes is: observable immediately after laser
action. The curves representing the_electrical_conditions_take_on_a_
"calmer" shape (form) and in the sick patients the muscle tone
decreases, a weakening comes . about.
It is neccessary to observe the presence. In nearly all the
patients on the 3rd and 11th day the presence of a reaction expressing
itself in the considerable increase of the intensity of electrical-
phenomenon, the increase of the amplitudes of muscular vibrations,
and intensification of hyperkinesis. These symptoms quickly disappear,
and towards the end of the course of treatment, diminishing of high-
frequency vibrations is observed, conditioned by muscular electro-
activity. The decrease in amplitude of these vibrations is accompanied
by a marked decrease of-hyperkinesis in the clinical picutre.
In this way, the modified skin galvanic reaction reflecting the
electrical condition of the organism. permits the oblique (indirect)
evaluation as to the degree of hyperkinesis dominance and permits
the objective evaluation of the therapy carried out.
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STIMULATION BY RADIATION.
THROUGH" ACUPUNCTURAL POINTS -11
Apart from that, data .can be found in literature pointing to the
connection between skin galvanie reaction-and-the-reticular formation
of the thalamus, hyperthalamus, and . the .sensorvarea-and cortex.
of the greater hemisphere .-. Consequently, by acting on the reticular
formation by means of radiating.the diencephal region with the help
of the method described, it is possible to control the reactiveness-
of the individual. Together with the registration of skin galvanic
reactions, indicators-of electroconductance of channels were taken
uniting the acupunctural points (microammeter M-95," with shunt,
electrodes Ag and Au). Data obtained are presented in table 5.
Caption of table: 5: . Changes in the electroconductance of channel
127a-122 (left leg, in microamps) during MKS therapy of children
suffering from cerebral paralyses.
-
The registration of electroconductivity wascarried out-until
treatment on the 1st, 3rd, 9th, and 11th days, and at the end of the
course of therapy (after 25 procedures).
1.1
On the third day (the sum duration of radiation lasting from
3-15 minutes) a decrease In . electroconductivity by from 1.5 to 2 times
was observed in comparison with the initial level. With the increase
-of-the-sum-dose-by-a factor-of 4-5-an increase-in the electroconduct-
ance index takes place on the 9th to 11th day which remains high_until
the end of treatment (1.5-3 times higher than the initial level) :
Of interest is the fact that the electroconductivity of channels
increases sharply by a factor of 2 after a single dose of laser
radiation and decreases below the initial level on the 3rd day.
After-threefold radiation; the increase-of-electroconductivity-is
again_threefold ( from call to 0. 34 mA), with the initial indices on
the 9th-11th day remaining at the same level . (0. 36 mA) . The appearance
of a sharp increase in conductivity . immediately after the laser
action and appearing within 5-10 minutes was observed when both
~ .~ small doses (30 sec) and large ones- (sum dosage towards the end
of the course-being from 34-134-minutes) -were applied. Such an increa
in electroconductivity is.apparently connected with_the_photoconductiv
effect.
-
...
Photoconductivity takes place when the photon energy of the
stimulating light rises above the significant threshold. The red
light energy in the waveband chosen by us is optimal for the
emission of electrons into the conductive-band.
On the basis of this it is possible_to_suggest_ that_the generation
of "free" charge carriers. and the occupation of electrons in the
conduction. band: takes place as a result of the action of laser-
radiation which brings about. an Increase -in electroconductivity. At.
the same time :the high indices remain at that: level (with slight
variations) over a period of 15 min after-radiation, which can be
explained by saturation of electrons in the conduction band.
The decrease in electroconductivity on the third day is
interpretable in 2 ways :. firstly, as the intensification of the-
recombination processes, decreasing the concentration of "free"
carriers, and secondly, as the reaction of the organism to the
increased doses which bring about an-arresting effect. The perpetuatic
- .
of the initial. level of electroconductivity_towards the_ 9th and . 11th
day is apparently connected with the establishment of new energetic
homeostasis .. . The level of conductivity is maintained throughout
all the periods of observation with the exception of a few increased
Indices immediately after the laser action and over a period of
5 minutes, therafter. The latter is typical of all cases, as has been
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Approved"
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Such stabilization of conductive indices of a definite level
which are maintained over a period from the 9th to Ilth day (during
all-periods-of observation), speak-for the attainment of a definite-
saturation threshold, that is to say, within the first lo-days, with
the increase of the sum (total) dosage, the electroconductive
indications increase te a definite limit. Concomitantly, an increase
In the intensity of physiological reactions takes place obtaining
Its maximum from the 9th to 11th day of procedure. Towards the
-25th procedure, a decrease in conductivity is observable, and after
the action by laser a 3-fold increase --- as compared with the initial
level --- is noted.
=
1. 1
-
This high level persists during all periods of observation,
which demonstrates the increase of. the energy resources of an organism
During treatment of factal nerve lesions in order to objectively
make diagnostie-tests-and-with the aim ofworking out individual
doeses_of_ laser radiation-a method_of_measuring-electroconductivity-
of channels uniting the facial acupunctural points was applied-
(B. Z. Shuyskaya, .T. M. Shakirova, 1972) .
For the purpose of searching for points and a numerical evaluatic
of electroconductive indicators, we utilized the above-mentioned
methods with the help-of-2 apparatus: M-265M a and the M-266M with
amplifier
All-in-all ll patients. underwent_therapy (lo children of ages
fro 7-14, and a 38 year-old ill. female patient) , among them 3
suffered from permanent damage caused by -poliomyelitis and one girl
with post-traumatic neurosis of the facial nerve. The etiological
moment in the remaining cases was connected with the chilling or
were inexplicable. _ The_dura-cton of the sickness-ranged-from 3-days-
to 19 years. Therapy was carried out with the aid of a light guide
trained on acupuncture points :
For the evaluation of the significance of electroconductivity,
healthy school children ages from 7 to 15 (54 persons) were observed
{checked)-for-a-norm."
The data obtained were processed using the variational statistica.
method (table-6). .
We discovered insignificant assymetries ef electroconductive
indicators ranging from I5 mA in the control groups. Thus, points
-25, 26, and 9 are weaker (from 4-7-MA) , and points 12, 22, and 23 have
-electroconductive indicators-(from-15-25-mA) .
Increasing differences_in_electroconductance-significances-are
observable. In children ages 7. to 11 the electroconductive indicators
are significantly higher than: in ages 12 to 15, which is apparently
explicable by age differences in the reaction of the nervous system an
receptivity of the child's organism to irritants .
In cases of facial nerve lesions, sharp asymmetries of electro="
conductive indices are observable, at the same time it is neccessary
during the process of therapy not only to be aware of their presence,
but above all, their magnitude, : degree of assymmetry, strength and-
distribution.
..
During the observation of children, a certain dependance of
the significance of electroconductance on the length of illness
and localization of the wound (trauma) was -apparent, however owing
to the few observations it is not possible to arrive at any definite
conclusions on this plane (stage) .
::
Nonetheless, there is -literature which shows the changes of
electrical indicat or (particularly resistances) during pathological
processes-which were used to determine the stage and localization of
the wound and to evaluate the effectiveness of the therapy carried-
out (A. K. Podshibyakin, 1962, V. L. Raykov, V. G .: Adamenko, 1968;
L.Ya. Mazo, 1969) .
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~Biostimulation through .. .. acupunctural points. - 11.
points in facial nerve lesions show that in recent forms of sickness
the conductive Indices on the damaged side in places of the locali-
zation of-the process-is much higher than on the healthy side of
-
the face_And_conversely, in-prolonged old-forms of-illness, the
electroconductance indices are_lower on the damaged side. This fact
led the authors . to speculate upon the problem of the selection of
Individual laser radiation dosages based upon the significance
of electroconductive indices. Firstly, it was indispensible to
decrease or increase the Indices of conductivity on the damaged side
of the face . depending upon: the initial level, secondly-to-
even out (equalize) . the assymetry, thirdly, to bring the electro-
conductive index nearer to the - norm. ..
To accomplish these tasks, careful measurements of electrocon-
-ductance of facial acupunctural points were made daily at the same
time of-day, up to the procedure and after it. Each point was
measured 3 times and-average results were taken. Observations-show-
that large doses of from 5-lo seconds on a point produce a decrease
in the significance of electroconductance by a factor of from 3-5.
Small doses (from I: -- 3 sec. on a point) increased the electroconduc
ance Indices by a factor of 1.5. - 3 (table 7) .
Thus, the skin galvancc reaction taken from the acupunctural
points reflects the human organism's reaction to the action-of
laser radiation. . Evidently, the electrical properties of the
acupunctural points can adequately mirror the states of the cortical
and subcortical centers of the brain (cerebrum) . Experiments have
"shown that the diameter of the points can vary from 1-8 mm
:
depending upon-the -physiological condition of the subject. On such-
a point, surface changes. in electrical properties-take-place,-a-
totally different reaction to the influence of light and the manifest-
ation of skin galvanic reaction.
What are the electrical and substational changes of acupunctural
points as opposed to the skin sectors not having these
"points, on
the activity of-He-Ne laser radiation? For-this-purpose,-experimental
research was carried out at a frequency of 50 Hz with the aid of-
-
an apparatus assembled according to a bridge design with Ag-AgCl
electrodes, the distance between them being -2 mm. " The electrodes
were given a potential of 0.75 V. To begin with, control measurements
of electroconductance -magnitudes were made, after which measurements"
were made over definite-time intervals-after radiation.
The topography of active points on the skin-of-a-frog-were
determined according to a scheme- (matrix): supplied by A. K. Podshibyaki
(1949) .
One of the symmetrical acupunctural points around the zone of
the ear situated on the head of the frog was subjected to laser
radiation beam of a diameter of-5 mk (microns) and-a-duration of
activity
of 2 minutes, with the light projection to other skin
sectors through a glass fiber light quide.
Vital (biological) stainings-were carried out in both symmetrical
sectors of the-skin ... The sorbtion dynamics was studied in the sector
containing the active point, as well as in the sector absent of
points (table 8).
The data obtained show that the acupunctural points are
characterized by: a lower level of sorbtion prior to radiation . After
a single radiation profound. substantial changes and
distinct phase.
reactions were observed :: ::
An hour after radiation, the sorbtion indicators returned to,
normal. Consequently, with the light intensity of.
-0.2 mW/cm
-and duration of action 2 minutes, the substantial changes
in the
active points are of an capo
8000 1.0
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BIOSTIMULATION_
through acupunctural points, -14 .
It is possible to assume that stimulation : gradually irradiates
in the joint system. The
electrical resistance after radiation
in the active points decreases sharply (from 30-50 % of the initial
-
level).
:
The D.C. electroconductivity of points-have a definite daily
rhythm. The minimum in the electrical conductivity was observed
:
:
at 2 AM and a maximum:at 6 PM. . "According to these data, the best
time to carry out the procedures is during the night when the
conductive systems of the whole organism possess a large reaction
capacity. - All the experiments carried out convince us about the
fact that acupunctural points react to light activity and through
them it is possible to influence a pathological nidus (focus of
infection) .
Up to the present time there is no agreement of opinion in
the literature about the primary processes-taking place in points
during needling.
Together with the mechanistic conceptions" about the therapeutic
action of inserting needles: fully substantiated opinion has been
extablished on the possible energetic nature of the primary effects
during the action of needling. (V.G. Vogralik, 1961).
As the above mentioned experiments show, changes take place in-
-a whole complex of electrical-properties: - resistance, charged
quantities, magnitudes_of_eggwhite_globules (albumin), membranes
and so on. : Consequently, the most fundamental thing about the primar
physical-chemical displacement (shift) "taking place in the point
during the action of laser light are the photo-electrical phenomena
which also change the degree of bioplasma mergy saturation which
-manifest themselves as initiators of acceleration-or-deceleration
-
of physiological_and biochemical processes
It is possible to suggest that the localized reaction to
"needling by light" takes-place- in the form of an axom-reflex mechani
Such a reaction .is expressible in various changes in the functional
conditions of the corresponding organs, depending upon the intensity
and duration of laser radiation action, that-is, during laser
radiation_action_on_an acupunctural_point, definite_bioenergeticalø
changes connected above -all
with the appearance of photo-currents
along the conductive channels and recharging of the membrane structur
will take place in the corresponding segment. A re-rediation ".
(secondary) effect is also possible in the bioplasma channels."
The general-raction of the organism develops-as -a-result-of-
the energy changes of the bioplasmas structures of cortex and
subcortical areas of the brain and the reticular formation. One
should not exclude the possibility of a dispersion of reaction
1 .
along the nerve conductors which have a bioplasmal level and
diverse (mani-fold) connections (for example, the triatic
(trigeminus) nerve).
There can be no doubt that when the skin is subjected to the
action of laser light-a reaction on the part of the suprarenal
takes place which :has a phased . character. (V.M. Inyushin, 197o) .
·
To conclude, we consider that it isneccessary to examine in
any illness -expecially of a neuro-dystrophic character is connected
with the bioenergy changes occurring in the whole organism which-
procede the biodynamic-shifts.
Pathology is above-all a change in the bioenergetic balance
of the bioplasma> the-disturbance.of its stability. From this,
..
a wholly systematic principle evolves which says that : ' there - are
no localized ailment --- all ailments are of a total character.
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BIOSTIMULATION.
through acupunctural points -15
Local-manifestations of ailments-exist,but-hardly-any -- focus-of-
the_maximal_expression_of_the most: distinct bioenergetic and
therefore also morphological changes. .
· 1
All kinds of patho-bio-energetical changes can accumulate over
a period of time up to a certain level which when reached are
observed to have secondary processes leading towards considerable-
-organic changes. .. Theconcept-of-G.Selle-about the role of "stress"
is also to be found in the framework of bio-energo=patho genesis.
Not only the nervous system but the whole energy system of the
organism is involved in a bottomless (infinite) chaos and
:
irreversability of chain reactions during the action of pathological
factors. - Disturbances of the resistance (stability) of the
bioplasma is in "stressed" situations_also-bring-about-so-called-
--
non-specific_symtoms_of_various_ailments, apparently it is neccessary
to introduce the understanding of bioenergetic "stress" which is
the cause of many. ailments .:
.
It is known that the concept of Sel'e ignores the role of the
-
-nervous-system in pathology-and-the-principle of-nervism in general .-
However, we know_of thousands_of_examples_showing-that-the-condition
of the nervous system and mental state determines the tendency of
the organism to illness ....
..
Painstaking experience and. the gradual training in overcoming
difficulties leads to the formation of a strong type of nervous
system. If-a person is kept away from activity; then the result is
the formation of a weak type of nervous system. What-is-the question
here? The conservation of internal energy means_the investing in _
health, on the contrary. After all; it is known that if a machine
is not in the process of working then it will last longer. With
the organism the case is contrary, in a state of inactivity a decrease
in energo-potential takes, place, resulting in a decrease in
resistance to illness
-
The concept of biplasma to_a certain_degree_explains_the
dependence of this . apparent paradox. The optimal level of
bioplasma. is conserved within definite bounds with its constant
injection of free particles. Injection is possible during the
particle exchange (during particle-exchange,-energy is released) -on-
account of mechanical, chemical, radiational, and other forms of
energy. "Thus, for example, when_a- muscle_is_contracted_not_only.
the transformation of the potential energy of bioplasma into
mechanical energy takes place, but as a result of the appearance
"during this of the piezoelectric effect, a formation of free charges
oceurs, which go into the bioplasma supply (storage) occupying a
definite location in this system:
The bioplasma, in conformity with our point of view, is
formed by particles -possessing various energy characteristics, in
other words, their energy potential. is of varied Significance (value) .
Such a varied composition is conditioned by different sources
resident in the bioplasmal constellations, at the same time the
injection of particles-is only possible during the action-of-some
kind of energy on the structure (chemical, mechanical, radiational,
and other forms. of energy) .
-
-
That is in fact why it is neccessary that the organism should
be in the sphere of activity of various. (differing) stimuli. The
-various stimuli also form a mosaic of particles differing in their
energy parameters and the formation of a specific bioplasma structure.
Without_the action of the stimulant, the organism weakens, as -a
result of homogenity in the energetic parameters of the particles
which unavoidably leads to : a structural disturbance of the initial
condition of the bioplasma ; and the appearance of instability.
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BIOSTIMULATION ..
. through acupunctural points -16
There can be no doubt that the "stress" situation brings about
quantitative and qualitative changes in the bioplasma of the whole
organism, which-in the final analysis leads to pathology .- We
suggest that the understanding of the concept of "stress" including
finding out about the bioenergetic interpretation will make it
possible to fuse the concept of Sel'e with the principles of nervism
At this level it is indispensible to go deeper into research since
the Sel'e thesis is one- sided, in actual fact, rejecting the role
of the nervous system in patho genesis;
-
Thus, for example, the development of all the components of
an inflammatory reaction, namely: the -widening (expansion) of
vessels (dilation), the intensfication of the permeability of the
Vascular tissue, and the appearance of secretion, etc, is the object
of the regular influence of the nervous system. This is wholly
understandable.since we-showed-above that the level-and-energy
saturation of_the_bioplasma assemblies_of _the_nervous_system. compared
to other biostructures, are considerably higher, hence the
possibility of attaining "an energetical dictatorship". It is
interesting to observe that patho-bio-energetic changes in the
internal organs unavoidably indicate energy shifts on the surface
of the human- skin: The walls of the body in-relation-to-the-internal
organs carry more negative charges, an exception_is_the_cephalic
brain and the nerve conduction channels (pathways) where a
.
. :
complicated mosaic of negative and positive assemblies put
together form a quasi-neutral state. In the human organism it is
possible to observe the presence of a preponderence of negative
"ectosome" (negative shells) and an inner, positive "nucleus".
(endosomes) . With the increase in the electropositiveness of the
internal organ, for example in the intensification of the inflammator
process, on the surface and united (joined). sectors and points,
zones with a large quantity of negatively charged magnitudes are
formed.
While-observing the electrical and quantum properties of the
skin surface, it is possible to judge where the localization_of
the inflammatory process is, and devise -preventive measures. .
The presence of chronic nidus inflammations can not only lead
to a decrease in the energy potential of the bioplasma, but also
-disturbs -the autoregulatory mechanisms of processes taking place
in the core of the bioplasma and linked-up- with-the-pathological.
wave. _ And_this leads to a-deformation of_the biofield loci, a
disturbance of the function, and finally the- appearance of
persistent morphological changes ...
.
Of great interest in this context are the so-called auto-
immunt illnesses. In such illnesses-the antigene homeostasis is
disturbed, as a result of which organosclerotic. manifestations
develop ..
According to the views of V. P. Kaznacheyev and others the
functional full value-of the immuneo-structural homeostasis determine
the occurrance and etiology (history) process of many illnesses.
If its function happens to be disturbed, then this may lead to two
extreme cases: - a) benign and malignant tumors; b) to distrophy and-
sclerosis ..
We suggest that the disturbances in the immuno-structural
homeostasis begin in the form of persistent -undamped changes in
the wave and quantum structure of the quantum biofield. These
changes take place under the influence of stress factors, for
example, during negative : emotions, functional overloading as
a result of the action of physical and chemical agents and the
influence of chronic inflammatory nidus. The latter brings about
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bioplasma constellations, located in the nuclei of the immuno-competer
cells.
:
The non-resistivity_of_ bioplasma is most.distinctly- - manifest
during changes in external factors, thus; with_the_presence of a
large number of positive ions in the surrounding air, the inflammatory
process in the organism progresses slowly owing to the large losses
of negative particles-and-can take on chronic form. The level of
energy resources of the sympathetic nervous system-decreases
sharply. In conditions of increasing positive-nature-of-the
atmosphere, the processes of infection take place more actively,
-
invading new areas. . With the presence of geomagnetic storms as a
result of disturbances of the solar plasma a disturbance of the
energetic connections takes place. In organisms with disturbed
resistivity, a disturbance-of-energy balance, an intensification of -
inflammatory phenomena and so on, takes place.
In this way in order to evaluate the bioenergetic_parameters.
of the patients - organism, it is neccessary to take into consideration
the electrical and magnetic condition of the environment at the
same time. It is necessary to know, that the reaction of the patient
organism can be of an opposite (converse) type, depending upon the"
initial condition_of_the_bioplasma_system in a manner-strongly-reactin
to shifts in the external environment, especially_during a patient's
illness.
-
:
-
-A doctor should bear in mind that the bioplasmic system in the
organism-is-united-and conditions the many different levels of
_energy changes in it during the appearance of a pathological -- nidus .-
Shifts occur, above all, in the_energy .parameters of the-central
and most concentrated organized sectors of the-bioplasmic_system:in:
the spinal brain, the ganglia, etc. . At the same time, these changes
can be both quantitative and of a space-time character. (Field time
changes).In some cases of chronic illness, the pathological changes
and their space=time: characteristics become persistent- (take: on --
resistive character) even when the nidus of the illness-is elimated .-
The long-term influence of the pathological impulses from the periphery
Can bring about the appearance of "local energetic focus" with an
increased potential of an opposite sign in relation to potential
of the neighboring areas in the bioplasma ensemble of the brain.
We suggest that this nidus can-be-seen primarily as positive
bioplasmic particles. Such an energy nidus_appears- as-a-sort- of
trap for the impulses and free particles forming, as a result of
the action of different .stimuli, .. on the functioning organism (such
an "attraction"; to an even greater degree, disorganizes the
-autoregulatory system of the organism).
.
-
Such is the bioenergetic interpretation of-the study of A.A.
Uhtomskiy's dominant. This great physiologist wrote: "In-connection-
with the formation of the dominant; the stimulatory energy . _ contributi
from the remaining centers- flow as if to it, and these latter ones
seem to be arrested (slowed down) as a result of their inability to.
react." The words of this prominent physiologist bear witness to
the necessity of discovering a-bioenergy mechanism of the appearance
of the dominant and its role in bio energo-patho genesis.
It is possible to suppose that. even A.A. Uhtomskiy_himself:
.
felt the necessity of having a bioenergetic interpretation of the
:
dominant, since by putting the question :- "is it neccessary to present
the dominant as a topographical single point in the central nervous
-system?"arrrhe answers this question in the following way:
"it (the dominant) manifests itself most probably as a definite-
--
concentration of centers of heightened sensitivity to various niveaus -
of the cephalic . and .. spinal brains, as well as the autonomic_system."
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BIOSTIMULATION.
through acupunctural points -18
This very well-coincides-with-our-conception-about-the-inevitable
compactness of bioenergetic changes in the interconnected loci of
the bioplasma system.
It is-known that the presentations on the occurrence of patholog
ical dominant lay at the foundation of the theory of pathogenesis of
-many illnesses. The removal of the dominant nidus, means finding
the key to the treatment- of-many illnesses ..
The methods of resonance biostimulation, as has been shown
above, paves the way to "extinguishing the dominant of pathological
niduses, and : their; return to : normal - status" ::
-
The residues of the sickness after an apparent recovery may be
the result of insufficiently effective therapy ... It is possible that
they are-conditioned-by-the consequent phenomena in the:bioplasmal
system, which are not always successfully removed by therapy and
which make the organism receptive to pathological shifts in the
presence of unfavorable factors In the environment.
The basic task of laser radiation therapy is the creation of
-an-energy "sub-vibration" (pumping) of the bioplasma substance of
the organism.as a result of which primarily_an improvement-in-the-
neurovascular trophic takes place:
The second task is the normalization of the quasi-neutral
condition of the bioplasma, that is, the reconstruction of its
stability (stationary state) or the conditions of its maximum"
persistence-(stabilization) of the bio energo stasis.
The third is indeed the most difficult task --- restoring to
their normal state.the space-time parameters of the bioenergetic
system of the whole organism. . This concerns the rhythms and wave
processes of the various frequency bands which it is neccessary to
normalize in case of pathological disturbance. This task has so far,
not-been possible to carry out due to absence of knowledge of the
above-mentioned parameters. in a normal. organism .-
In the case of sharply. decreased bioenergetic organism balance ..
the lability of the-bioenergetic parameters in connection with the
bioplasma's lack.of persistence, it is neccessary to effect a
-
"bloenergetic pumping" (sub-vibrations) for the purpose of creating
an energetical basis for the further morphophysiological normalization
of the organism of a patient.
The normalization of trophic-interrelationships in the organisms
and the restoration of conductive channels is one of the main
directions taken in bioenergy therapy, and here the method of laser
radiation action on acupunctural points has a great future.
-In order to carry out such a complex operation as the normalizatic
of the bioenergetic status of the organism through_acupunctural_points
it is indispensable to make a very accurate diagnosis of the energy
condition of the organism_ Namely,- from the evaluation of the
Initial condition of the anisotrophic . field of the organism ( and
here, the acupunctural points are one of the distinct manifestations
of-such-anisotropy) it is necessary to begin therapy-using biotic-
agents. And here, an invaluable aid may turn out-to-be-the-development
of electronic calculating techniques: With their aid, it would be
possible to obtain information on the innumerable bioenergetic
conditions of acupunctural. points .. A certain experience in this
sphere has been-accumulated by. V. A. ruschov and N.C. Dzevitskaya.
Research has-been carried out on a few patients with chronic
tonsilitis. Measurements-were made of-the-electroconductivity-of-15-
sectors of the skin surface of the human face. On a special model
the magnitudes of electroconductivity were fixed in a dertain definite
order with the aid of potentiometers ... The most probable recipe for
the activity o
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BIOSTIMULATION ..
through acupunctural points -19
of the indicator lamps, which topographically are connected to the
various sectors of: the human face. With the sharpening . (intensification
of the process, the activity of definite points increases. During
the normalization of the process, the probability of mxmaximum-
electroconductivity decreases. Consequently, with the aid of an
analog computer setup, it is possible to check the dynamics of laser
:
therapy.
.
..
-
. .
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*1

Total 10: 24 , our dynamics
of the intensity of electrobiolum-
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(Or ) 1
28
26
24
22-
20
18
15
14
12
10
6
4
2
t
10
12
=
20
22
24 2
.
-
-
10
ЧАСЫ СУТОК
Рис. 10. Суточная динамика интенсивности
электробиолюминесценции кожи уха кролика.
Diagram 4: X-rays of the radius
bones of dogs, 15th day after fractu:
fa) control group b) stimulation by
laser radiation
4740795
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Рис. 4. Рентгенограммы лучевой кости собак на 15-е сутки "
перелома: а - контроль, 6 - стимуляция лазерным излучение
1900 À
2800 Å
3300 Å
У.ф. диапаза
UV wavebar
6200 Å 6800 Å
Красный
Juanazon
red wavebar
Радиоволны
WWWW radio wa
acoustical and
infrasonic wave
+
O11
+++
+ exciton and eleo
- hole plasma
• Протонная плазма
Sect Approved Fordeleaset2000/08/07 : CIA-RDP96-00787R000500080004ton plasma
The numerous membranes are visible.
Fig 6: Bioplasmal system in cell
and the dominating frequencies
Участок цитоплазмы
hydrodynamic
plasma waves
®
Fig 5: Electron microphotograph.

=
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CONCLUSION
Interest in bioenergetics is_at_present:very great .- It-is-in -.
this science that one sees-a sort of "savior"' fromthe cold formalis
of cybernetics the extraordinary amount of hypotheses in the sphere
"of molecular biology, the wild flowering of medical therapy, the
increase of the preponderance of degenerative Illnesses with various
symptoms. In the theoretical area of bioenergetics a synthesis
of the manifoldness -of :phenomena "is expected,-phenomena-obtained-
in the process of large experimental work -- many_experiments.
1. .
-
411
:
In our book we-have shown that the bioenergetic approach_to the
problem of therapy and pathogenesis of an illness is fruitful, since
what we are concerned with is that we should treat the whole organss
above all, as a. bioenergetic-whole. The organism possesses a
considerable energy: reseave-which can be utilized-in its-fight-with --
an ailment making use-of- very weak energy phenomena with the aid
of laser radiation .:
Bioenergetics also requires the revolutionization (revision)
of-many of our-concepts about. the living organism as a whole.
"Namely, in the sphere_of_bioenergetics-is-concealed-'this biologica.
bomb'which will in the end enable scientists to reevaluate -- the-
present state of affairs as a new historical_and_logically indispens
Ible revolutionary stage in the development of the science of life,
that highest form on our planet.". These words of the famous
academician-B.A. Dombrovskiy are neccessarily the best the underline
the significance of bioenergetics for the qualitatively new
understanding of life in all its forms.
. :
Science is fertile :when it has further (long-range)_ perspective!
and when it makes a positive contribution in practice. And here,
-we- come across the first successes-of bioenergetics in both-
medicine-and-agriculture-(1).
At_the same time, the-approach-to-the. utilization of laser
radiation in the given plan, is not doubly empirical,but -- is-based-
on the foundation of .definiate theoretical assumptions_(hypotheses) ,
such as the concept. of bioplasma; - etc .. Intensive work has begun
to decipher. the mechanism of the activity of coherent electromagnetic
radiations of lew intensity on -different levels of living
organisation of the organism, which-we wrote about in the foregoing"
chapters.
Ending the presentation of material, we consider it neccessary
-
in a generalized way to throw- light upon the initial (primary)
-mechanisms of the activity of HeNe laser light which in the final
stage-brings-about a therapeutic effect.
During .our investigations we hit-upon-an-assumption: do laser
photons not bring about changes in_the_ qualitative parameters-of-the-
energy structure of the organism? In this context, it is
Interesting to look at A. G. Gurvich's idea that: the photon with
specific characteristics introduces a st-Fictly determined metabolica
process and- determines-the ".
.space-time existence of
the process. . Such assumptions are vindicated-by the large amount of-
experimental material on the study of the properties-of-mitogenetic-
radiation.
It is possible to assume that such a high effectiveness of
-hyper-weak mitogenetic radiation depends upon a whole series of
unique wave properties of living. organismal radiations, above all,
the_high degree of-monochromaticity of such radiation, Its coherence"
and polarization .: These properties create-such a-basis, thanks-
to which it is.possible to induce resonance effectsin the bioplasma-
and biomolecules-with the aid of optical quantum generator radiation.
Every point of the blofield has discrete elements which are
reduced by the regulating influences of the whole. The biofield
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4

.
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CONCLUSION-2:
the parametrical quantum amplifier. Amplification in the form of
a cascade process- takes place only as a result of radiation with
definite wave properties. .. It is possible, and herin Ites the secret,
that-during small (low) intensity-mitogenetic-radiation in the tissue.
a considerable.macroeffect-takes-place.
-
One of these macroeffects is the increase in the permeability
of cells during the action of mitogenetic radiation, the stimulation
of cell fragmentation (mitogenesis). Mitogenetic radiation
stimulates mitosis even in the frozen cornea of the eye of a frog.
The introduction of so-called-mitogenetic suppressives bring
about a prevention of cell division. Consequently, the proliferative
activity is regulated with the aid of radiation of strictly
defined parameters:
Already attempts have been made to model the mitogenetic radiati
with very weak intensity sources of non-coherent and depolarized
-ultraviolet- radiation. It-was shown that the effectiveness-of-
ultraviolet action is observable at a maximum of 2800-A-and-somewhat
lower in the area of about 2500 A. At the same time, the_stimulaTING
effect of the mitogenetic radiation is cut off approximately at
wavelength 2700 A, and.in darkness and at supplementary lighting by
Infra-red or visible light, displacement towards a more long-wave
area of the specturm -.
(3250-+-60 A). However, an identical-
effect_was not_obtained . in the-case of - noncoherent, -depolarized
ultraviolet radiation ...
.
=
It is neccessary .to explain the possibility of imitating
mitogenesis with the aid . of. laser sources.
There are reasons to
assume that mitogenetic rays possess properties similar to laser-
-- radiation .- Possibly this is the secret of its high-effectiveness.
The presence in the cell of structures possessing_semiconductive
properties speak of the possibility of the generation of a laser-
type radiation. An-ignition of the mitogenetic radiation is
observed during .. "energy pumping .. by a weak, constant electrical
current, visible light, and Infra-red radiation. Consequently,
the living cell can work like a self-constructed-biosemiconductive
laser. This is one of the suggestions. _ Another is also possible:
mitogenetic radiation is generated by-liquid crystal_structures of
the living systems .: " At the same time, the presence of coherent
auto-radiation. basically causes-one to assume the possibility of
resonance effects.
-We are in the possession of-convincing data- - about-the-fact-
that during the action of activity in waveband :SVCh (visible light
frequency) a resonance effect is observed: An so, the-bacteriologica.
(bacterocidal). effect of SVCh- (visible light) frequency is
observable on strictly determined frequencies, if the frequency is
changed, the absence of the effect is observed (V.G. Adamenko, 1968,
N. D. Devyatkov, 1970), Analogous phenomena are also recorded
during the action of SVCh frequency effects on hemoglobins
(L.G. Koreneva, V.I. Gayduk, 1970 et al. )
Proceeding from the representation of the bioplasma as an
organized semiconductive system, we-assume that the resonance effects
of radiation can appear at their maximum during a space-time
correspondence of radiation with the wave and time structure of-
the biofield.
Weak radiations possessing strictly determined parameters can
show distinctly expressed macroeffects; as is shown by the mitogeneti
radiation .. .
The creation of lasers in different spectral bands creates the
basis for their influence upon the endogenous ray processes with
the aid of coherent electromagnetic radiations.The regenerative
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CONCLUSION--3

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chemical agents or by unspecified stimulants. At present we have
almost approached the possibility of regulating proliferation
by low intensity characteristic radiations. Although one
encounters great difficulties on the part of creating a desired
regulation, they are not insurmountable.

The concept of bioplasma, presented above, opens new possibiliti[es]
for the development of the study of bioresonance and its application
to medical problems. The real possibility exists of a direct
indication of bioplasma radiation which can find application as a
way of early diagnosis of illnesses, as also the reaction of the
organism to specific irritants.

In connection with this is is desirable to observe the work of
Prof. P.I. Gulyaev, from the Leningrad State University who with
the help of a volume-data machines fixes the electrostatic field
around a pulsing heart, a stimulated nerve, etc (2).

In the diagram below, we have attempted to picture the possible
mechanism of resonance biostimulation by laser radiation.

Bioplasma is the most reactive system to the action of light
in the red area of the spectrum. At the same time, atoms, molecules,
and radicals interact with the laser radiations by emitting electrons
in the zone of bioplasma conductivity.

The primary mechanisms of light action is "the energy pumping" o[f]
the bioplasma with electrons and holes of determined energy stimulatio[n]
(in the range 1.2 electron volts). The red, short-wave light is
the most effective for such bioenergetic pumping. By accumulating
2 photons, the biosystem is able to radiate them in the form of
mitogenetic photons (thus, the energy of 2 photons.wavelength
6328 A is equal to the mitogenetic quantum of wavelength 3264 A ).

At the biophysics faculty of the Kazakh State University, it
was possible to I.B. Beklemishev, V.A. Semikin, and K.K. Tleubayev
to show in an experiment the appearance of UV harmonics during the
action of various living tissues and plants by HeNe-lasers. At
the same time, cells having the greatest intensity contained
clorophyll. All measurements were carried out with the aid of
photoelectron apparatus (FEU-42) with luminescent screen-

Utilizing the photographic non-contact method of recording
mitogenetic radiation, a co-worker of the faculty in question,
L.A. Kiryeev, registered the ignition of a secondary radiation in
the area 3100-3300 A from the tissue of a green leaf during the
action of light coming from a HeNe-laser. Consequently, the fact
of generating quantum energy in the UV area of the spectrum, that is,
mitogenetic radiation during the action of red light gas lasers,
was proved. The part of the photon migration of mitogenetic
radiation along the conductive channels of the whole organism was
shown in the work of the successors of A.G. Gurvich. This is the
source from which an understanding was obtained of the stimulation
effects of low-intensities and exposure on the regenerative bone
tissues, peripheral boils, and inflammations.

Namely, the secondary radiation also brings about a changes in
the permeability of cell membranes, including also capillaries
which also are of great significance for the resolution of
inflammatory processes. The appearance of superfluous charges on
the surfaces of membranes brings about the activization of the
reticular endothelia (see diagram on facing page).

It is not excluded that the secondary radiation brought about
by laser red light creates conditions for the improvement of inter-
cullular tissue and organ contacts, that is created
the increase of homeostasis in all its manifestations (immuno-homeostasis,
nerve-emotional, etc.)

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CONCLUSION
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These primary processes, taking place in the bioplasma and the
biomolecular assemblies- indicate the whole series of macroeffects:
the appearance of energy homeostasis of the nervous system; and
all other systems of autoregulation, because of the disturbance
whose resistivity is the unbalancing of the bioplasms, changes of
membrane surface properties and their pathological activity in
connection with the increase-or decrease of the optimal level_of
bioplasma.
:
-
The electrical state (condition) of membranes, their permeabilit
which can be normalized by acting on the bioplasmal system with the
aid of laser light. The stimulation of the changable process is
conditioned by the electrical displacements in the membranes,which
change their catalytic properties.
Acceleration of the acid-creative processes takes place apparent
on account .. of. the-activization of the cytochromes of the mitochondri
Namely, In these organelles the basic creation and accumulation of
-
energy in the cells takes place.
The experimental data_given in the book have-demonstrated-the
fact of the presence of specific reactiveness, not only_depending
upon the frequency of radiation; but its polarization and -
coherence .. Polarized and coherent radiation possesses more resonant
effect on biological structures. In connection with the discovery
. 1
of-such-resonance effects-a quite-new aspect of utilizing laser
techniques . in biology and medicine has appeared .- In-this-werk -- only
the_physiological.effectiveness of the short-wave-part of the
spectrum has been shown .- There is- no doubt about the fact that
resonance lines also exist in other bands of the
electromagnetic
spectrum, for whose discovery further research Is neccessary.
All of the experimental work-has-been-based on concepts about --.
the_semiconductive structure of the living organism-and-in -connection
with this, the photo-electric phenomena -taking place in the tissues
in the presence of light. ..
At the same time It is necessary to learn about the peculiarity
of the physiological action of modulated light of a Helium-Neon
laser, functioning according to a determined program on a living
organism.
It is possible to suggest that stricter_requirements to determin
parameters of the radiation agents during : its action on a living
system with the purpose of inducing physiological resonances will
be neccessary. All the experimental material gathered In the work
-presented-confirms-this attitude .- In-this book it -is-shown that it
is possible to utilize radiation generated by HeNe-lasers-in-the-
formot a stimulator: The exposures -applied manifested a stimulation
effect on eritopoesis, the post-traumatic regneration of the skin, an
synthesis of glycogen," The stimulation, as is usual; is of a
parabolic. character. The maximum of the stimulating effect Is
observed from the 3rd to the 17th day, after this, a characteristic-
fall on the part of the organism takes place. which had beensubjected
to radiation action ....
The initial bioenergetic: condition of the radiated organ, or
the whole organism determines its reaction on the activity of the
stimulator. Radiation on the background of hyperfunction brings
-about a decrease in reaction, and conversely, in the case of hypo-
function, its increase.
The diagnosis of the initial condition should be the most
constant area of interest: ta.biophysics. Knowledge about the initial
condition_will .make,it_possible to more accurately make a prognosis
of the direction which the physiological reaction during. action of
-
-.
-a-radiation stimulator will take.
The basic results obtained in the process of our theoretical
and experimental work can be formulated in a few statements:
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Conclusion-5 -- -
1) spectral lines in the short-wave-band of the red part of the
spectrum_(6300-6500 A)_have-been-discovered_possessing_physiological
resonance effects ..
:
2) polarization increases-the physiological effectiveness (efficienc
of radiation which makes lasers the most suitable sources of light
-
for the stimulation of biological processes. (corpuscular formation
post-traumatic-regeneration-of-skin,etc.)
3) He-Ne laser radiation does not bring about profound organic chang
in skin tissues; organs of corpuscular formation, and endocrinal
glands, and quickens, or slows down the physiological processes
depending upon their .initial. state. :
This can be used or for purposes of normalizing, a whole series-
of pathologieal processes which already are being carried out-in
practice in clinics.
Experience, amassed in the clinics of Alma-Ata and other towns
show that non-medicinal therapy with the aid-of resonance biostimulat
ion in certain cases is considerably more effective than the best
chemo-therapeutic agents, and in fact, do not create complications.
At the present time, experiments are-also being-made to find
other ways_of_developing_optical_quantum generators. At_the_same time,
attention is drawn to the role of secondary radiations of the
bio-substratum for biostimulation.
It appears that real possibilities exist for the creation of a
physical model of the biofield for fine (precise) regulation of many
-physiological-processes-of-the-organism, including those of the
cephalic brain, It is not neccessary- to prove the value of the
results of such work for medicine, psychology, and agriculture.
The bioenergetics of the whole organism is on the rise. We hope
that its achievements will serve to the benefit of the health of
man, his physical and spiritual development.
In conclusion; we consider it essential to briefly dwell on some
interpretations of the Kirlian effect which_appeared recently in
foreign publications.
Thus, for example, the gas discharge luminesence taking place
around the human finger and its light effects was called bioplasma;
when the works of Soviet researchers were referred to in-the-
foreign press. - This is-in principle incorrect_In_our_work_we
have_not_once-shown_that_the_Kirlian effect_is an_oblique_method of
uncovering some of the properties of bioplasma. - We showed that the
effect itself: of the light from the gas discharge plasma within a
certain period of time, is accompanied by bioplasmal Iumthesence, as
a result of ion electron bombardment.". The Intensity of this
luminesence-in-the- best-of-cases, does not comprise more than 15% of
the whole light stream registered during the action of high
voltage discharge:"
Bioplasma possesses its own light (luminesence) but it is very
0
weak under normal physiological conditions. The structure of this
luminescence-was demonstrated by us using the non contact
bioradiographic method, without supplementary influencing. During
the Kirlian effect, we are able to observe a luminous ignition of
bioplasma amplified a billion times on account of the electron-
ion bombardment:
..
A significant part is also played by auto-electron emission in
the formation of luminesence and image formation (especially in
cases of utilizing luminopher or photoemulsion). For this reason,
V.G. Adamenko:has presented quite significant demonstrations_published
in scientific literature. In connection with this, one is filled
with disbelief: when confronted with the opinion of the American
--
scientist W. Tiller (In Psych
the physiological condition of-
Approved For Release 2000/08/09
ng object, and that all displacements in the luminesence
1

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Conclusion-6

take place as a result of the deformation of the empty space between
the bio-object and the photoemulsion.

According to our viewpoint, the data obtained by W. Tiller are
an example of the other extreme, when the researcher, not having
understood the substance (essential point) of the Kirlian effect has
completely lost that 12-15 % of useful information about the
bio-object, which was present in the macro and micro streamers of the
gas discharge over a short space of time. Apparently, enthused by
the physical experiments, Dr. Tiller did not succeed in fixing
this information and at the same time lost the nucleus of the Kirlian
effect.

Only on the basis of quantitative evaluation of luminesence as a
result of high-voltage discharge is it possible to obtain reliable
information about the processes taking place in plants and man. This
is substantiated by the numerous works about the Kirlian effect,
presented in a series of scientific publications. The diagnostic
value of the Kirlian effect does not arouse doubt. This method is
one of the finer ones of evaluation the bioenergetic displacements
(changes) in the whole organism. At the same time, it is also
neccessary to accept the presence of "noise" information in the
gas discharge, which as our research has shown can be differentiated
(distinguished). One would like to pause here to mention a series
of experiments which show that luminesence which takes place on
the streamer contact (object) carries information of the physiological
condition.

Thus, when the living or plant object is heated, an intensive
light discharge is observed during illumination. During illumination
of a leaf with light the phenomenon of charge polarization takes place
and luminesence in the shaded sector drops.

Under the action of a magnetic field on plant and living tissue,
the effect of a reduction of luminesence takes place which persists
over an extended period of time, even in the case when the magnet
does not directly act upon the living tissue.

The inhibition of breathing brought about by poisoning brings
about a reduction of the luminesence of roots and leaves of plants.

Finally, one should not forget to mention some quite interesting
experiments made in the Alma-Ata institute of medicine by Prof.
A.R. Rahishev and collaborators. They discovered the changes of
intensity of luminesence of palatal knot in cats.

The undamaged knot had a more distinct luminesence (2-3 times)
than in the case when it was decentralized as a result of cutting. It
was further shown that short laser discharges significantly changed
the intensity of luminesence (1.5-2 times). All the experiments
were carried out using lumiphor screens, the significance of whose
light intensity was determined by a photoelectron magnifier. The
apparatus was developed at the biophysics faculty of the Kazakh
State University.

Without doubt, the Kirlian apparatus is far from perfection, and
is one of the few accessible means for diagnosing bioplasma. At
the same time, with the help of the Kirlian effect, it was possible
to penetrate into a new field of knowledge, utilizing the most recent
achievements of quantum electronics to obtain unique information
about the bioenergetic state of the organism, and prove the existence
of the biofield.

The work carried out in our laboratory demonstrates the
possibility of obtaining information about biofields without supplementary
influence (action) on the living organism. Footnotes :

[Footnote]
1) V.M. Inyushin and M.N. Fedorava, "Experience in the utilization of
lasers in a series of technical techniques", 12th issue, 1970
p.p. I. Gulyaev, etal, "Electroaurograms of the human and a mammal"
The Nervous System, Isvestin LGU, 9th edition, 1968

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UCIS DEPARTMENTS ONLY
SECRET
DI
A
G
D
M
OF
IT
it
E
M
E
C
-
H
N/
1
SM
0
ה
L
ASER
R
A
D
T
0
N
TH
E
R
A
Y
A
IV
1
E/W
ED
ACCORD
1
N
G
TO
T
H
C
N
E
P
T
0
IF
B
OPLASMA.
Monochromatic
cohérent polar
-izing electro
- magnetic
radiation
Bloplasma of
the organism .
Resonance effe
ffects,
of
Increase in the!
intensity
mitoticic ra
- didtion with
the succeeding.
normalization
of the intensity
level.
0
Stimulatory
wave of cell
division
( mitosis)
Acceleration of
regeneration,
desensibilization,
increase in
resistance to
infections dis
- leuses removal
of pathological
Omenalice, norma
- lization of
hormonal balance,
bettermen
Normalization
of permeation
of cell membranes
Tendency towards
normalization
of immune
homeostasis
won't of the
neutofcellular
tropic
The normalization
of the energy.
balance of bio
- plasma in the
nervous and
other autoregw
- factory structure
-es!
Intensification
of oxidation
regenerative
processes in
the cells and
organs
Stabilization
of herve
régulation
- processes
-
1.9
. .
-
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-
A
-
P
S
0
C
increase or de-
crease in the
density of
bioplasma,
occurence of
secondary radi
-ation.

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-
Table 8: Sorbtion differences of neutral red in the active points in
the skin sectors of a frog. Without active pts. during LASER radiation in
Post radiation time:
1.
corresponding units.
objects
control
30 sec
5 min
10 min
1 hour
active
84.6
115.0
113.0
110.0
80.0
points ...
+8.1
±13.0
+11.2
+11.5
+7.7
sectorg
114.7
134.0
114.5
109.0
120.0
without active pts.
+10.5
+14.6
+16.0
+10.4
+5.8
TAME I physical characteristics_of_soviet_lasers_used for
biological research
RADIATION SOURCE
TYPE
OUTPUT in mW
weight, Kg
OKG-12_shock_&
multimode
20
16
. J
vibration resistant
CW
ONG -13 shock &
multimode
CW
0.2
1
vibration resistant
LG-75
HE-Ne
multimode
CW
25
28
gas_laser
LG-36A
monomode
40
LG-36a
multimode
.80
40
1
Notes: CW= continuous wave, i.e. constant operation
OKG = optical quantum generator, i.e. laser
Table
Pigment
Control
mg%
Polarized
mg
chloraphyll a
100.0
120.5
chloraphyll b
62.8
70.0
keratin
3.0
3.2
lutin
2.0
3.2
violaxatin
1.6
1.5
sum of the
green pigments
125.0
166.5
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COSIB DEPARTMENTS (RC
=
.. . ..
1

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[Typed body]
Table 3: The content of pigments in leaves:-* 4-day growth, mg o/o.

Control                                    Polarization

clorophyll "A"   100.0                     120.5     [Struck through: "100.0"]
clorophyll "B"   62.8                      70.0
karotin           3.0                       3.2
lutine            2.0                       3.1
biolaxatin        1.6                       1.5      [Struck through: "1.6" and "1.5"]
Sum of the green
pigments          125                       166.5     [Struck through: "125"]

Table 5: Changes in the electroconductance of channel 127a-122
(left leg, in microamps) during MKS therapy of children suffering
from cerebral paralyses.

Procedure        Total Dose            Initial data                    After radiation
                                                                        by LG-75

1st        30 sec to 5 min    o.18  o.11  o.22  o.52       o.35  o.38  o.31  o.42
3rd        3.5 min to 15 min  .41   o.19  0.31  0.22        0.34  o.27  0.24  0.31
9th to 11th  15- 63 min       o.36  0.31  0.30  0.37        0.52  0.58  0.38  0.39
25th        54 - 134 min      0.26  0-35  0.45  0.49        0.55  0-54  0.59  0.49

[Column headers, vertical text]
starting level | after 5 min | after lo min | after 15 min | immediately after radiation | after 5 min | after lo min | after 15 min.

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.
-
Table 6: Mean indices of electroconductance channels
joining the acupunctural points in children (norm)
Age of children
No. of point
right
background
left-
-background
9
9.2+0.5
1.7
8.7+0.4
1.9
:
TO
10.2+0.3
1.6
10.8+0.6
1.9
12
25.1+0.9
3.8
20.4+0.5
3.6
from 7-11 years
23
18.3+0.7
3.5.
25
6.5+0.3
1.3
6.8+0.3
1.5
26
5.9+0.3
1.3
5.9+0.3
1.2
103
6.5+0.3.
2.2
6.9+0.3
1.1
.
9
7.1+0.5
1.5
7.2+0.5
1.3
10
9.2+0.9
1.6
8.3+0.6-
1:0
12
12.5+0.9-
3.0
10.9+0.6-
2.8
22
15.1+0.7_
3.1
14.7+1.0
3.5
23
14.4+0.7
3.5
--
from 12-15
25
5.4+0.7
1.3
6,7+0.9
1.5
years
26
4.7+0.7
1.5
5.5+0.7
1.0
TO3
7.6+0.6
1.0
7.4+0.5
1.2
3
9.6+0.8
7.0
29
9.6+0.7
1.0
33
5.7+0.4
1.1
91
5.1+0.3
0.8
5.7+0.3
0.9
113
4.8+0.3
0.7
4.9+0.3
0.9
TO7
6.7+0.4
0.7
8.4+0.7
0.9
D. L .- Parmanenko (1960)
Remarks: The point numbers are given according to Fin-Li-Da.
Table 7: Electroconductive indices of active facial points when
the facial nerve has been cut.
Points
prior to procedure
exposure 10 sec
"after procedure
right
-left
right
left
-
right
left
9
15
30
3
5
20
20
10
20
23
3
4
32
10
12
60
30
5
3
22
42
22
23
23
3
30
40
25
12
13
3
13
47
4
26
10
30
3
4
10
13
USIB DEPARTMENTS ONLY
: =
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:
22
25.010.8
4.9
24.0+0.6
4.2.
1
:

- Approved For Release-2000/08/07 -:- GIA-RDP96-00787R000500080001-0 --
SOVIET BIBLIOGRAPHY-OF-LITERATURE-ON-RESONANCE -- BIOSTIMULATION
{as-contained-in-the-original -- Russian-manuscript)
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:
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Inyushin, V.M. : "K veprosu;o_biologicheskey_aktivnosti krasnoy radiatsii"
Alma-Ata, 1965 con
the biological activity of red
Approved For Release 2000/08/07 : CIA-RDP96-00787R000500080001-0
.. .

Approved For Release 2000/08/07 : CIA-RDP96-00787R0005000800011-U
SOVIET . BIBLIOGRAPHY --- 2.
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monochromatic red light from lasers.
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,
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.
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Approved ForRelease 298910
and Medicine, Alma-ata 1969)
USIR REPERTUEL

Approved For Release-2000/08/07 . CIA-RDP96-00787R000500080001-0
BIOGRAPHY ON RESONANCE BIOSTIMULATION -3
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i aashchita. „Tezisi dokladov respublikanskovo simpoziuma, Kiev,
izd-vo Naukova dumka, 1969. (On.some physiological and biochemical"
reactions in the organism brought about by laser radiation. The biol.
action of lasers( dosimetry, appl-in-med,-and protection) .- Thesis-
of lectures given_at_Republic_symposium_Kiev ;_ scientific_thought
publishers, 1969)
St .- D'erdri A. : "Vvedeniye-v submolekulyarnuyu biologiyu. M, Nauka, 1964
(Introduction to sub-molecular biology, .Moscow, science, 1964)
"Bioenergetika." M., 1964: (Bioenergetics, Moscow 1964)
Shakirova T.M. : "opit- lecheniya monohromatichskim krasnim-svetom-detey
s_tserebral nimi paralichami ._ V.sb. : Nekotoriye_voprosi_biodinamiki_
i bioenergetikiiorganizma: v_ norm i patologii; biostimulyatsiya
-
lazernim izlucheniyem: :. Alma-Ata,. 1973 . (Morphological changes in
the bone marrow and spleen under the influence of monochromatic
red light. In the colle on the biol. action of MRL, Alma-Ata, 1967)
-Shchur-V. V., Makyeyeva, N. S. : "K-voprosu o-rezultatah lecheniya monohroma-
ticheskom krasnim svetom gelli-neonovo lazera. arterial' noy_gipertonii
i nekotorih neurologicheskih zabolevanii. V sb: Nekotoriye voprosi
biodinamiki i.bioenergetiki organizma v norme i patologii, biostimuly-
atsiya lazernim. 17luchen yem. Alma-Ata: 1972. (On the question of the
results of mil He-Ne laser treatment of arterial hypertony and some
neurological ailments., In the coll
Approved For-Release 2006/08/67 :TOMARDR96.00.687R00050008000 4-0amics-and
state, Alma-ata)
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SECRET
SOVIET BIBLIOGRAPHY .ON RESONANCE"BIOSTIMULATION=4:
Shchur V. V., Makyeyeva N.S. , Arenberg A. A., Bolts M. V., Nikanorov Yu.A:
"Primeneniye kvantovovo generatora-(lazera) pri lechenti ran. Vestnik
chirurgii imeni I. I. Grekova, Meditsina, 1972, No. 6, 85-89.
(The utilization of laser QG in the treatment of wounds. Surgical cour-
ier, named after I.I. Grekov, Medicine, 1972, Nr. 6, 85-89}
-
:
Shuyskaya B.z., Shakirova r.M .: "Modifitsirovannaya kozhno-gal vanichesk-
aya reaksiya u detey s tserebral nimi paralichami pri lechenti svetom
gelit-neonovovo lazera. V.Sb. ": "Svet gelli-neonovih lazerov v biologii
i meditsine. Alma-Ata, 1970. (Modified skin galvanic reaction in
children suffering from cerebral paralysis, in the coll: some questions
of biodynamics and bioener. of org., in normal and pathological states)
Smirnova Ye.P .: "Morfologicheskiye izmeneniya kostnovo mozga i selezenki
pod vityantem monohromattcheskovo krasnovo sveta. V-sb: "0 biologiches-
-kom-deystvii-monochromaticheskove-krasnovo-sveta. Alma-Ata,-1967 :-
(Morphological changes in the bone marrow and spleen under the
influence of MRI. In the coll: on the biol. action of MRL, Alma-ata)
Stepanov B. I. , .. Rubinov.A.N .: "Neprerivno perestraivayemiy lazer na
krasitelyah. Priroda, 1973, No. 9. ( Continuously variable dye lasers
Science, 1973,-Nr. 9)
-Vel'hover-N.S .: "O signal'ney funktsii ushnoy rakovini- Yezhegodnik
nauchnih rabot Alma-Atinskovo insituta usovershenstvovaniya vrachey,
t. 3, 1967. (On the signal function of the ear shell. Annual of
scientific works of the Alma-Ata institute of qualifying doctors,
Vol 3, 1967/1
Vogralik V.G. : "Osnovi kitatyskovo lechebnovo metoda Chjen'tszyu. Kor kiy,
1960. (Foundations of the Chinese-healing-method-Tsien-tsu-Gorky)
Vvedenskiy N.Ye. ; "Izmeneniye_funktsional!nih.svoystv_nerva pri evo
narkotirovanii. Sobr. soch., 1953. ( The change of functional propert-
ies of the nerve during narcosis. Collected works, 1953)
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BIBLIOGRAPHY OF RELATED RESEARCH COMPILED BY THE TRANSLATION EDITORS
COPENHAGEN 1976

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[Typed body]
Kovacs,I, Mester E., and Görög, P. :"Stimulation of wound healing by
  laser rays as Estimated by means of the Rabbit Ear chamber Method"
  Acta Chirurgica Academiae Scientarium Hungaricae,Tomus 15 (4),427-432
Mester, E, Bácsy, E., Spiry, T and Tisza, S: "Laser stimulation of
  wound healing--enzyme-histochemical studies" Ibid,Tomus 15(2),203-08
  (1974)
Mester,e, A. Korényi-Both, Spiry,T. and Tisza, S: "Laser und muskelfase
  Regeneration (vorlaufige mitteilung)"Ibid, Tomus 15 (4),337-342
Gurwich,A.G. and Gurwich, LD: "Die mitogenetische Strahlung",
  Gustav Fischer Verlag, Jena (1959)
Gurvich, A.A. "Problems of Mitogenetic Radiation as an Aspect of
  molecular biology" Meditsina, Leningrad (1968)
Rahn, O : "Invisible Radiation of Organisms", Borntraeger, Berlin,1936
Konev, S.V.: "Flourescence and Phosphorescence of Proteins and Nucleic
  Acids" (English Trans) Plenum Press, New York (1967)
Barenboim, g.m., Domanskii, A.N. and Turoverov,K.K. "Luminescence of
  biopolymers and cells"(english trans)Plenum Press, New York (1969)
Metcalf, W.S. and Quickenden, T.I. Nature, 216,169 (1967)
Doman, M.J. "A study of Luminescent processes in Aqueous Media with
  reference to Bioluminescence" Doctoral Dissertation, University of
  new york at Buffalo, (1968)
Barnes, Frank :"Application of Lasers to Biology and Medicine" Proc.
  of the IEEE, vol 63, Nr. 9, 1975
Czerski, Przemyslaw: "Experimental Models for the Evaluation of Micro-
  wave biological effects" Proceedings of the IEEE,Vol 63,No. 11,
  Nov. 1975, pp. 1540-1543
Tromp, Solco W: "Psychical Physics" Elsevier, Amsterdam 1949
Hill, Scott: "Acupuncture Research in the USSR" American Journal of
  Chinese Medicine,1976 , Vol , Nr , pp.
Hill, Scott: Letter to the editor on the Kirlian electrophotography
  technique, Journal of the Society for Psychical research, London,
  March 1975, pp. 58-60

------------------------

the following articles are being translated by the Danish S.P.R.
in Copenhagen and will be available shortly. They supplement the
material in this monograph:
Beyasheva, Z.G., Bekmuhambetova, B.A.:"Investigation of the influence
  of red and white polarized light and the light of HeNe laser on
  EEG of man" Proceedings of the second thematic symposium of scientific
  practical papers on the problem of "Psychical Self Regulation",1974
Gushja, A.Z, Shvald, P.G., Semionkin, E.I., Slivin, S.I.:"Treatment
  of trophic ulcers, unclosing wounds and inflammatory infiltrations
  by monochromatic red light" Ibid
Dzevitskaja, M.T. "About a method of calculation of recipies of
  acupuncture according to electroconductivity of the skin integument"
  Ibid, pp. 325-29
Inyushin, V.M. Inyushin and Kireeva, L.A. "Bioradiography--a method
  of indicating the biological field" Ibid, pp. 338-343
Inyushin, V.M., Beclemishev, I.B., Semikin, V.A., Tleubasev,K.K.:
  "About the effect of ultraviolget radiation by living tissues in
  interaction with red light"" Ibid, pp. 336-338
Shugarov, N.A., Voronkov D.V.: "Ossesous tissue restoration in
  treatment by intromedullary osteosynthesis combined with the influence
  of laser radiation" Ibid,

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